7XA3: CCL2 bound CCR2-Gi complex
Cryo-EM structure of the CCL2 bound CCR2-Gi complex. Determined by electron microscopy at 2.9 Å resolution. Released 24 Aug 2022.
- Method
- Electron microscopy
- Resolution
- 2.9 Å
- Organism
- Homo sapiens
- Chains
- 6
- Atoms
- 9,591
- Mol. weight
- 164.29 kDa
- Released
- 24 Aug 2022
Explore 7XA3 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7XA3 contains 37 α-helices and 65 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 9 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-31 | 25 | |
| β-strand | 34-40 | 7 | 3 |
| α-helix | 46-55 | 10 | |
| β-strand | 185-190 | 6 | 3 |
| β-strand | 195-200 | 6 | 3 |
| α-helix | 212-215 | 4 | |
| β-strand | 220-226 | 7 | 3 |
| β-strand | 233 | 1 | 4 |
| α-helix | 234 | 1 | |
| β-strand | 241 | 1 | 4 |
| α-helix | 242-254 | 13 | |
| β-strand | 263-269 | 7 | 3 |
| α-helix | 271-280 | 10 | |
| α-helix | 283-285 | 3 | |
| α-helix | 296-309 | 14 | |
| β-strand | 319-323 | 5 | 3 |
| α-helix | 328-351 | 24 | |
Chain B: 5 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-30 | 23 | |
| α-helix | 35-38 | 4 | |
| α-helix | 39-41 | 3 | |
| α-helix | 43-44 | 2 | |
| β-strand | 52-56 | 5 | 5 |
| β-strand | 63-68 | 6 | 6 |
| β-strand | 74-79 | 6 | 6 |
| β-strand | 83-88 | 6 | 6 |
| β-strand | 94-99 | 6 | 6 |
| β-strand | 105-110 | 6 | 7 |
| β-strand | 116-121 | 6 | 7 |
| β-strand | 125-130 | 6 | 7 |
| β-strand | 139-145 | 7 | 7 |
| α-helix | 150 | 1 | |
| β-strand | 151-156 | 6 | 8 |
| β-strand | 161-166 | 6 | 8 |
| β-strand | 171-175 | 5 | 8 |
| β-strand | 181-185 | 5 | 8 |
| β-strand | 192-197 | 6 | 9 |
| β-strand | 203-208 | 6 | 9 |
| β-strand | 213-217 | 5 | 9 |
| β-strand | 223-227 | 5 | 9 |
| β-strand | 234-239 | 6 | 10 |
| β-strand | 245-250 | 6 | 10 |
| β-strand | 255-259 | 5 | 10 |
| β-strand | 264-269 | 6 | 10 |
| β-strand | 278-283 | 6 | 11 |
| β-strand | 289-294 | 6 | 11 |
| β-strand | 298-303 | 6 | 11 |
| β-strand | 309-314 | 6 | 11 |
| β-strand | 320-325 | 6 | 5 |
| β-strand | 332-336 | 5 | 5 |
| β-strand | 341-344 | 4 | 5 |
Chain C: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-23 | 17 | |
| α-helix | 30-43 | 14 | |
| α-helix | 45-47 | 3 | |
Chain E: 6 helices, 24 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-5 | 4 | 12 |
| β-strand | 9-11 | 3 | 13 |
| β-strand | 17-24 | 8 | 12 |
| α-helix | 28-30 | 3 | |
| β-strand | 32-38 | 7 | 13 |
| α-helix | 43 | 1 | |
| β-strand | 44-50 | 7 | 13 |
| β-strand | 57-59 | 3 | 13 |
| β-strand | 67-72 | 6 | 12 |
| β-strand | 77-82 | 6 | 12 |
| α-helix | 87-89 | 3 | |
| β-strand | 91-98 | 8 | 13 |
| β-strand | 110 | 1 | 13 |
| β-strand | 114-118 | 5 | 13 |
| β-strand | 140-141 | 2 | 14 |
| β-strand | 146-147 | 2 | 15 |
| β-strand | 155-161 | 7 | 14 |
| β-strand | 166 | 1 | 16 |
| β-strand | 172 | 1 | 16 |
| β-strand | 174-179 | 6 | 15 |
| α-helix | 184-185 | 2 | |
| β-strand | 186-190 | 5 | 15 |
| β-strand | 194-195 | 2 | 15 |
| β-strand | 203-207 | 5 | 14 |
| β-strand | 211-216 | 6 | 14 |
| α-helix | 221-223 | 3 | |
| β-strand | 225-231 | 7 | 15 |
| α-helix | 237 | 1 | |
| β-strand | 238-239 | 2 | 15 |
| β-strand | 243-245 | 3 | 15 |
Chain L: 1 helix, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 25-31 | 7 | 2 |
| β-strand | 40-45 | 6 | 2 |
| β-strand | 50-53 | 4 | 2 |
| α-helix | 58-68 | 11 | |
Chain R: 13 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 35-69 | 35 | |
| α-helix | 76-93 | 18 | |
| α-helix | 95-101 | 7 | |
| α-helix | 110-143 | 34 | |
| α-helix | 145-151 | 7 | |
| α-helix | 154-178 | 25 | |
| β-strand | 179-184 | 6 | 1 |
| β-strand | 187-192 | 6 | 1 |
| α-helix | 196-207 | 12 | |
| α-helix | 208-212 | 5 | |
| α-helix | 213-231 | 19 | |
| α-helix | 235-268 | 34 | |
| α-helix | 277-302 | 26 | |
| α-helix | 303-307 | 5 | |
| α-helix | 310-322 | 13 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Isoform B of C-C chemokine receptor type 2 | R | protein | 360 | Homo sapiens | P41597 (AlphaFold model) |
| C-C motif chemokine 2 | L | protein | 69 | Homo sapiens | P13500 (AlphaFold model) |
| Guanine nucleotide-binding protein G(i) subunit alpha-1 | A | protein | 354 | Homo sapiens | P63096 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 365 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | C | protein | 71 | Homo sapiens | P59768 |
| scFv16 | E | protein | 256 | Homo sapiens | |
Sequence of entity 1 (R), FASTA
>7XA3_1 Isoform B of C-C chemokine receptor type 2 (chains R)
MLSTSRSRFIRNTNESGEEVTTFFDYDYGAPCHKFDVKQIGAQLLPPLYSLVFIFGFVGN
MLVVLILINCKKLKCLTDIYLLNLAISDLLFLITLPLWAHSAANEWVFGNAMCKLFTGLY
HIGYFGGIFFIILLTIDRYLAIVHAVFALKARTVTFGVVTSVITWLVAVFASVPGIIFTK
CQKEDSVYVCGPYFPRGWNNFHTIMRNILGLVLPLLIMVICYSGILKTLLRCRNEKKRHR
AVRVIFTIMIVYFLFWTPYNIVILLNTFQEFFGLSNCESTSQLDQATQVTETLGMTHCCI
NPIIYAFVGEKFRRYLSVFFRKHITKRFCKQCPVFYRETVDGVTSTNTPSTGEQEVSAGL
Sequence of entity 2 (L), FASTA
>7XA3_2 C-C motif chemokine 2 (chains L)
QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWV
QDSMDHLDK
Sequence of entity 3 (A), FASTA
>7XA3_3 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF
Sequence of entity 4 (B), FASTA
>7XA3_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAM
HWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNIC
SIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFT
GHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAF
ATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALK
ADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWRL
FKKIS
Sequence of entity 5 (C), FASTA
>7XA3_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 6 (E), FASTA
>7XA3_6 scFv16 (chains E)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGSSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQ
Primary citation
Molecular insights into ligand recognition and activation of chemokine receptors CCR2 and CCR3. Shao, Z., Tan, Y., Shen, Q. et al. Cell Discov (2022) 8:44-44. DOI 10.1038/s41421-022-00403-4 · PubMed
Other PDB entries of the same protein (UniProt P41597 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7P8X 1.4 Å, Crystal Structure of leukotoxin LukE from Staphylococcus aureus in complex with a doubly…
- 6GPX 2.7 Å, Crystal structure of CCR2A in complex with mk-0812
- 5T1A 2.81 Å, Structure of CC Chemokine Receptor 2 with Orthosteric and Allosteric Antagonists
- 6GPS 3.3 Å, Crystal structure of CCR2A in complex with mk-0812
- 2MLO Human CCR2 Membrane-Proximal C-Terminal Region (PRO-C) in a Membrane bound form
- 2MLQ Human CCR2 Membrane-Proximal C-Terminal Region (PRO-C) in a frount bound form
Browse structure collections
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