7XHN: Human inner kinetochore CCAN-DNA complex
Structure of human inner kinetochore CCAN-DNA complex. Determined by electron microscopy at 3.71 Å resolution. Released 25 Jan 2023.
- Method
- Electron microscopy
- Resolution
- 3.71 Å
- Organism
- Homo sapiens
- Chains
- 20
- Atoms
- 23,364
- Mol. weight
- 772.18 kDa
- Released
- 25 Jan 2023
Explore 7XHN in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7XHN contains 128 α-helices and 81 β-strands across 17 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain c: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 262-266 | 5 | |
Chain H: 9 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 40-65 | 26 | |
| α-helix | 78-81 | 4 | |
| α-helix | 83-89 | 7 | |
| α-helix | 91-116 | 26 | |
| α-helix | 125-145 | 21 | |
| α-helix | 147-187 | 41 | |
| α-helix | 195-209 | 15 | |
| α-helix | 219-222 | 4 | |
| α-helix | 233-236 | 4 | |
Chain I: 32 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 63-75 | 13 | |
| α-helix | 85-99 | 15 | |
| α-helix | 103-111 | 9 | |
| α-helix | 123-128 | 6 | |
| β-strand | 137 | 1 | 1 |
| α-helix | 139-145 | 7 | |
| α-helix | 158-170 | 13 | |
| β-strand | 175 | 1 | 1 |
| α-helix | 178-182 | 5 | |
| α-helix | 185-189 | 5 | |
| α-helix | 190-192 | 3 | |
| α-helix | 198-208 | 11 | |
| α-helix | 211-213 | 3 | |
| α-helix | 216-228 | 13 | |
| α-helix | 233-244 | 12 | |
| α-helix | 269-281 | 13 | |
| α-helix | 370-378 | 9 | |
| α-helix | 382-401 | 20 | |
| α-helix | 402-404 | 3 | |
| α-helix | 410-428 | 19 | |
| α-helix | 433-442 | 10 | |
| α-helix | 452-458 | 7 | |
| α-helix | 471-475 | 5 | |
| α-helix | 487-513 | 27 | |
| α-helix | 530-550 | 21 | |
| α-helix | 555-565 | 11 | |
| α-helix | 572-575 | 4 | |
| α-helix | 585-592 | 8 | |
| α-helix | 597-620 | 24 | |
| α-helix | 632-636 | 5 | |
| α-helix | 637-650 | 14 | |
| α-helix | 686-694 | 9 | |
| α-helix | 716-725 | 10 | |
| α-helix | 729-736 | 8 | |
Chain K: 7 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-45 | 25 | |
| α-helix | 55-74 | 20 | |
| α-helix | 90-146 | 57 | |
| α-helix | 155-182 | 28 | |
| α-helix | 184-187 | 4 | |
| α-helix | 209-222 | 14 | |
| α-helix | 240-248 | 9 | |
| β-strand | 253-254 | 2 | 2 |
| β-strand | 262-263 | 2 | 2 |
Chain L: 13 helices, 20 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 28-39 | 12 | |
| β-strand | 47-48 | 2 | 3 |
| α-helix | 49 | 1 | |
| α-helix | 61-68 | 8 | |
| β-strand | 72 | 1 | 4 |
| β-strand | 74-77 | 4 | 5 |
| α-helix | 78-79 | 2 | |
| β-strand | 80 | 1 | 6 |
| α-helix | 88-104 | 17 | |
| β-strand | 118-123 | 6 | 5 |
| α-helix | 134-136 | 3 | |
| β-strand | 137-142 | 6 | 5 |
| β-strand | 156 | 1 | 5 |
| β-strand | 160-163 | 4 | 5 |
| α-helix | 168-171 | 4 | |
| β-strand | 179 | 1 | 6 |
| β-strand | 183-185 | 3 | 5 |
| α-helix | 190-204 | 15 | |
| β-strand | 208-209 | 2 | 5 |
| α-helix | 215-225 | 11 | |
| β-strand | 237 | 1 | 7 |
| β-strand | 238-242 | 5 | 8 |
| β-strand | 251-253 | 3 | 8 |
| β-strand | 256 | 1 | 7 |
| α-helix | 258-268 | 11 | |
| β-strand | 274-275 | 2 | 3 |
| α-helix | 277-295 | 19 | |
| β-strand | 297 | 1 | 9 |
| α-helix | 299-301 | 3 | |
| β-strand | 303-308 | 6 | 8 |
| β-strand | 315 | 1 | 8 |
| β-strand | 321 | 1 | 4 |
| α-helix | 328-343 | 16 | |
Chain M: 7 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4 | 1 | 10 |
| β-strand | 15 | 1 | 11 |
| β-strand | 17-21 | 5 | 12 |
| α-helix | 26-37 | 12 | |
| β-strand | 43 | 1 | 11 |
| β-strand | 47-48 | 2 | 12 |
| β-strand | 63-68 | 6 | 12 |
| α-helix | 76-82 | 7 | |
| α-helix | 85-87 | 3 | |
| β-strand | 88 | 1 | 10 |
| β-strand | 97-98 | 2 | 12 |
| β-strand | 99-101 | 3 | 13 |
| α-helix | 114-123 | 10 | |
| β-strand | 128-130 | 3 | 13 |
| α-helix | 136-139 | 4 | |
| α-helix | 143-153 | 11 | |
| α-helix | 162-166 | 5 | |
Chain N: 12 helices, 16 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-13 | 8 | |
| α-helix | 21-27 | 7 | |
| α-helix | 33-38 | 6 | |
| α-helix | 45-57 | 13 | |
| α-helix | 63-75 | 13 | |
| β-strand | 83-89 | 7 | 14 |
| α-helix | 101-115 | 15 | |
| β-strand | 120-122 | 3 | 14 |
| β-strand | 125 | 1 | 14 |
| β-strand | 130-137 | 8 | 14 |
| β-strand | 145-155 | 11 | 14 |
| β-strand | 160-164 | 5 | 14 |
| α-helix | 172-182 | 11 | |
| β-strand | 187-195 | 9 | 14 |
| α-helix | 198-206 | 9 | |
| β-strand | 238-239 | 2 | 12 |
| α-helix | 242-251 | 10 | |
| β-strand | 267-269 | 3 | 15 |
| β-strand | 272-273 | 2 | 16 |
| β-strand | 291-292 | 2 | 16 |
| β-strand | 293 | 1 | 7 |
| β-strand | 295-297 | 3 | 15 |
| β-strand | 298 | 1 | 8 |
| α-helix | 302-307 | 6 | |
| α-helix | 310-312 | 3 | |
| β-strand | 315 | 1 | 9 |
| α-helix | 321-324 | 4 | |
| β-strand | 334 | 1 | 15 |
Chain o: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 17-26 | 10 | |
9 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Centromere protein O | O, o | protein | 300 | Homo sapiens | Q9BU64 (AlphaFold model) |
| Centromere protein H | H | protein | 253 | Homo sapiens | Q9H3R5 (AlphaFold model) |
| Centromere protein I | I | protein | 756 | Homo sapiens | Q92674 (AlphaFold model) |
| Centromere protein K | K | protein | 269 | Homo sapiens | Q9BS16 (AlphaFold model) |
| Centromere protein L | L | protein | 344 | Homo sapiens | Q8N0S6 |
| Centromere protein M | M | protein | 180 | Homo sapiens | Q9NSP4 |
| Centromere protein N | N | protein | 345 | Homo sapiens | Q96H22 |
| Centromere protein P | P | protein | 288 | Homo sapiens | Q6IPU0 |
| Centromere protein S | S | protein | 138 | Homo sapiens | Q8N2Z9 |
| Centromere protein T | T | protein | 561 | Homo sapiens | Q96BT3 |
| Cenp-W | W | protein | 88 | Homo sapiens | Q5EE01 |
| Centromere protein X | X | protein | 81 | Homo sapiens | A8MT69 |
6 more molecules are not listed.
Sequence of entity 1 (O, o), FASTA
>7XHN_1 Centromere protein O (chains O, o)
MEQANPLRPDGESKGGVLAHLERLETQVSRSRKQSEELQSVQAQEGALGTKIHKLRRLRD
ELRAVVRHRRASVKACIANVEPNQTVEINEQEALEEKLENVKAILQAYHFTGLSGKLTSR
GVCVCISTAFEGNLLDSYFVDLVIQKPLRIHHHSVPVFIPLEEIAAKYLQTNIQHFLFSL
CEYLNAYSGRKYQADRLQSDFAALLTGPLQRNPLCNLLSFTYKLDPGGQSFPFCARLLYK
DLTATLPTDVTVTCQGVEVLSTSWEEQRASHETLFCTKPLHQVFASFTRKGEKLDMSLVS
Sequence of entity 2 (H), FASTA
>7XHN_2 Centromere protein H (chains H)
MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLLRLRAQTKQQLLEYKSM
VDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKKLALDRMRLSTALKKNLEKIS
RQSSVLMDNMKHLLELNKLIMKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKN
KQKIDLDSMENSERIKIIRQNLQMEIKITTVIQHVFQNLILGSKVNWAEDPALKEIVLQL
EKNVDMMHHHHHH
Sequence of entity 3 (I), FASTA
>7XHN_3 Centromere protein I (chains I)
MSPQKRVKNVQAQNRTSQGSSSFQTTLSAWKVKQDPSNSKNISKHGQNNPVGDYEHADDQ
AEEDALQMAVGYFEKGPIKASQNKDKTLEKHLKTVENVAWKNGLASEEIDILLNIALSGK
FGNAVNTRILKCMIPATVISEDSVVKAVSWLCVGKCSGSTKVLFYRWLVAMFDFIDRKEQ
INLLYGFFFASLQDDALCPYVCHLLYLLTKKENVKPFRVRKLLDLQAKMGMQPHLQALLS
LYKFFAPALISVSLPVRKKIYFKNSENLWKTALLAVKQRNRGPSPEPLKLMLGPANVRPL
KRKWNSLSVIPVLNSSSYTKECGKKEMSLSDCLNRSGSFPLEQLQSFPQLLQNIHCLELP
SQMGSVLNNSLLLHYINCVRDEPVLLRFYYWLSQTLQEECIWYKVNNYEHGKEFTNFLDT
IIRAECFLQEGFYSCEAFLYKSLPLWDGLCCRSQFLQLVSWIPFSSFSEVKPLLFDHLAQ
LFFTSTIYFKCSVLQSLKELLQNWLLWLSMDIHMKPVTNSPLETTLGGSMNSVSKLIHYV
GWLSTTAMRLESNNTFLLHFILDFYEKVCDIYINYNLPLVVLFPPGIFYSALLSLDTSIL
NQLCFIMHRYRKNLTAAKKNELVQKTKSEFNFSSKTYQEFNHYLTSMVGCLWTSKPFGKG
IYIDPEILEKTGVAEYKNSLNVVHHPSFLSYAVSFLLQESPEERTVNVSSIRGKKWSWYL
DYLFSQGLQGLKLFIRSSVHHSSIPRAEGINCNNQY
Sequence of entity 4 (K), FASTA
>7XHN_4 Centromere protein K (chains K)
MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETLTDSNAQLSLL
IMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKLRQDLEMVLSTKESKNEKLKE
DLEREQRWLDEQQQIMESLNVLHSELKNKVETFSESRIFNELKTKMLNIKEYKEKLLSTL
GEFLEDHFPLPDRSVKKKKKNIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWP
PYVELLLRNGIALRHPEDPTRIRLEAFHQ
Sequence of entity 5 (L), FASTA
>7XHN_5 Centromere protein L (chains L)
MDSYSAPESTPSASSRPEDYFIGATPLQKRLESVRKQSSFILTPPRRKIPQCSQLQEDVD
PQKVAFLLHKQWTLYSLTPLYKFSYSNLKEYSRLLNAFIVAEKQKGLAVEVGEDFNIKVI
FSTLLGMKGTQRDPEAFLVQIVSKSQLPSENREGKVLWTGWFCCVFGDSLLETVSEDFTC
LPLFLANGAESNTAIIGTWFQKTFDCYFSPLAINAFNLSWMAAMWTACKMDHYVATTEFL
WSVPCSPQSLDISFAIHPEDAKALWDSVHKTPGEVTQEEVDLFMDCLYSHFHRHFKIHLS
ATRLVRVSTSVASAHTDGKIKILCHKYLIGVLAYLTELAIFQIE
Sequence of entity 6 (M), FASTA
>7XHN_6 Centromere protein M (chains M)
MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNR
PRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKL
AHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
Sequence of entity 7 (N), FASTA
>7XHN_7 Centromere protein N (chains N)
MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQRKESVVQHLIHLCEEKR
ASISDAALLDIIYMQFHQHQKVWEVFQMSKGPGEDVDLFDMKQFKNSFKKILQRALKNVT
VSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYSQTPYAFTSSSMLRRNTPLLGQALT
IASKHHQIVKMDLRSRYLDSLKAIVFKQYNQTFETHNSTTPLQERSLGLDINMDSRIIHE
NIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLNGSILAEREEPLRCLIKFSSP
HLLEALKSLAPAGIADAPLSPLLTCIPNKRMNYFKIRDKHHHHHH
Sequence of entity 8 (P), FASTA
>7XHN_8 Centromere protein P (chains P)
MDAELAEVRALQAEIAALRRACEDPPAPWEEKSRVQKSFQAIHQFNLEGWKSSKDLKNQL
GHLESELSFLSTLTGINIRNHSKQTEDLTSTEMTEKSIRKVLQRHRLSGNCHMVTFQLEF
QILEIQNKERLSSAVTDLNIIMEPTECSELSEFVSRAEERKDLFMFFRSLHFFVEWFEYR
KRTFKHLKEKYPDAVYLSEGPSSCSMGIRSASRPGFELVIVWRIQIDEDGKVFPKLDLLT
KVPQRALELDKNRAIETAPLSFRTLVGLLGIEAALESLIKSLCAEENN
Sequence of entity 9 (S), FASTA
>7XHN_9 Centromere protein S (chains S)
MEEEAETEEQQRFSYQQRLKAAVHYTVGCLCEEVALDKEMQFSKQTIAAISELTFRQCEN
FAKDLEMFARHAKRTTINTEDVKLLARRSNSLLKYITDKSEEIAQINLERKAQKKKKSED
GSKNSRQPAEAGVVESEN
Sequence of entity 10 (T), FASTA
>7XHN_10 Centromere protein T (chains T)
MADHNPDSDSTPRTLLRRVLDTADPRTPRRPRSARAGARRALLETASPRKLSGQTRTIAR
GRSHGARSVGRSAHIQASGHLEEQTPRTLLKNILLTAPESSILMPESVVKPVPAPQAVQP
SRQESSCGSLELQLPELEPPTTLAPGLLAPGRRKQRLRLSVFQQGVDQGLSLSQEPQGNA
DASSLTRSLNLTFATPLQPQSVQRPGLARRPPARRAVDVGAFLRDLRDTSLAPPNIVLED
TQPFSQPMVGSPNVYHSLPCTPHTGAEDAEQAAGRKTQSSGPGLQKNSPGKPAQFLAGEA
EEVNAFALGFLSTSSGVSGEDEVEPLHDGVEEAEKKMEEEGVSVSEMEATGAQGPSRVEE
AEGHTEVTEAEGSQGTAEADGPGASSGDEDASGRAASPESASSTPESLQARRHHQFLEPA
PAPGAAVLSSEPAEPLLVRHPPRPRTTGPRPRQDPHKAGLSHYVKLFSFYAKMPMERKAL
EMVEKCLDKYFQHLCDDLEVFAAHAGRKTVKPEDLELLMRRQGLVTDQVSLHVLVERHLP
LEYRQLLIPCAYSGNSVFPAQ
Sequence of entity 11 (W), FASTA
>7XHN_11 CENP-W (chains W)
MALSTIVSQRKQIKRKAPRGFLKRVFKRKKPQLRLEKSGDLLVHLNCLLFVHRLAEESRT
NACASKCRVINKEHVLAAAKVILKKSRG
Sequence of entity 12 (X), FASTA
>7XHN_12 Centromere protein X (chains X)
MEGAGAGSGFRKELVSRLLHLHFKDDKTKVSGDALQLMVELLKVFVVEAAVRGVRQAQAE
DALRVDVDQLEKVLPQLLLDF
Primary citation
Structural insights into human CCAN complex assembled onto DNA. Tian, T., Chen, L., Dou, Z. et al. Cell Discov (2022) 8:90-90. DOI 10.1038/s41421-022-00439-6 · PubMed
Other PDB entries of the same protein (UniProt Q9BU64 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 28OP 2.7 Å, Structure of the human inner kinetochore CCAN and CENP-C bound to DNA
- 7R5S 2.83 Å, Structure of the human CCAN bound to alpha satellite DNA
- 7PKN 3.2 Å, Structure of the human CCAN deltaCT complex
- 7XHO 3.29 Å, Structure of human inner kinetochore CCAN complex
- 9TAW 3.54 Å, Structure of the human inner kinetochore CCAN bound to DNA
- 7PB8 3.68 Å, Crystal structure of the CENP-OPQUR complex
- 9TAX 4.5 Å, Structure of the human inner kinetochore CCAN bound to a mono-CENP-A nucleosome
- 7R5V 4.55 Å, Structure of the human CCAN CENP-A alpha-satellite complex
- 7QOO 4.6 Å, Structure of the human inner kinetochore CCAN complex
- 7YYH 8.9 Å, Structure of the human CCANdeltaT CENP-A alpha-satellite complex
- 7YWX 12.0 Å, Structure of the human CCAN CENP-A alpha-satellite complex
- 9TAY 15.5 Å, Structure of the human inner kinetochore CCAN bound to a di-CENP-A nucleosome
Browse structure collections
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