7XOU: CCK-8

Structural insights into human brain gut peptide cholecystokinin receptors. Determined by electron microscopy at 3.2 Å resolution. Released 20 Jul 2022.

Method: Electron microscopy
Resolution: 3.2 Å
Organisms: Homo sapiens, synthetic construct
Chains: 6
Atoms: 8,247
Mol. weight: 156.61 kDa
Released: 20 Jul 2022

Explore 7XOU in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XOU contains 27 α-helices and 47 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	13-37	25
β-strand	40-46	7	1
α-helix	53-58	6
β-strand	208-214	7	1
β-strand	217-223	7	1
α-helix	237-239	3
β-strand	243-248	6	1
α-helix	265-277	13
β-strand	286-291	6	1
α-helix	294-301	8
α-helix	313-317	5
α-helix	332-352	21
β-strand	359	1	1
α-helix	371-387	17

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
β-strand	47-51	5	2
β-strand	58-61	4	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
α-helix	95	1
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	135-140	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	176-180	5	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	217-223	7	6
β-strand	229-234	6	7
β-strand	241-245	5	7
β-strand	249-254	6	7
β-strand	259-265	7	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-21	13
α-helix	30-43	14
α-helix	44-47	4
α-helix	53-55	3

Chain N: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	10-11	2	10
β-strand	17-25	9	9
α-helix	29-31	3
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	59-60	2	10
β-strand	70-73	4	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-98	7	10
β-strand	118	1	10
β-strand	122-125	4	10

Chain R: 10 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	44-68	25
α-helix	75-93	19
α-helix	96-104	9
α-helix	111-143	33
α-helix	155-172	18
β-strand	179-182	4	11
β-strand	195-198	4	11
α-helix	203-217	15
α-helix	220-243	24
α-helix	302-338	37
α-helix	351-369	19
α-helix	376-384	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
CCK-8	L	protein	9	Homo sapiens	P06307 (AlphaFold model)
Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	380	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	384	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	126	synthetic construct
Cholecystokinin receptor type A	R	protein	428	Homo sapiens	P32238

Sequence of entity 1 (L), FASTA

>7XOU_1 CCK-8 (chains L)
DYMGWMDFX

Sequence of entity 2 (A), FASTA

>7XOU_2 Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSV
MNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSD
QDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVASSSYN
MVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEKVLAGKSKIEDYFPE
FARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRR
VFNDCRDIIQRMHLRQYELL

Sequence of entity 3 (B), FASTA

>7XOU_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGS
SGGGGSGGGGSSGVSGWRLFKKIS

Sequence of entity 4 (G), FASTA

>7XOU_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (N), FASTA

>7XOU_5 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTV

Sequence of entity 6 (R), FASTA

>7XOU_6 Cholecystokinin receptor type A (chains R)
MDVVDSLLVNGSNITPPCELGLENETLFCLDQPRPSKEWQPAVQILLYSLIFLLSVLGNT
LVITVLIRNKRMRTVTNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYF
MGTSVSVSTFNLVAISLERYGAICKPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYS
NLVPFTKNNNQTANMCRFLLPNDVMQQSWHTFLLLILFLIPGIVMMVAYGLISLELYQGI
KFEASQKKSAKERKPSTTSSGKYEDSDGCYLQKTRPPRKLELRQLSTGSSSRANRIRSNS
SAANLMAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTASAERRLSGTPISFILLLSY
TSSCVNPIIYCFMNKRFRLGFMATFPCCPNPGPPGARGEVGEEEEGGTTGASLSRFSYSH
MSASVPPQ

Primary citation

Structural insights into human brain-gut peptide cholecystokinin receptors. Ding, Y., Zhang, H., Liao, Y.Y. et al. Cell Discov (2022) 8:55-55. DOI 10.1038/s41421-022-00420-3 · PubMed

Other PDB entries of the same protein (UniProt P06307 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9BKK 2.51 Å, Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
9BKJ 2.59 Å, Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex
7EZM 2.9 Å, Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gq complex
7EZK 3.1 Å, Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gs complex
8IA7 3.1 Å, Structural insights into human brain gut peptide cholecystokinin receptors
7EZH 3.2 Å, Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gi complex

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