7Y27: SST-14-bound SSTR2-miniGq-scFv16 complex

Cryo-EM structure of the SST-14-bound SSTR2-miniGq-scFv16 complex. Determined by electron microscopy at 3.48 Å resolution. Released 19 Oct 2022.

Method: Electron microscopy
Resolution: 3.48 Å
Organism: Homo sapiens
Chains: 6
Atoms: 8,184
Mol. weight: 140.23 kDa
Ligands: CLR
Released: 19 Oct 2022

Explore 7Y27 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7Y27 contains 33 α-helices and 58 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-50	4	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	88-94	7	2
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
α-helix	129-131	3
β-strand	134-139	6	3
β-strand	146-151	6	4
β-strand	156-161	6	4
β-strand	165-170	6	4
β-strand	178-180	3	4
β-strand	187-190	4	5
β-strand	200-203	4	5
β-strand	207-210	4	5
β-strand	220-222	3	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	250-254	5	6
β-strand	259-264	6	6
β-strand	276-278	3	7
β-strand	284-288	5	7
β-strand	293-298	6	7
β-strand	304-309	6	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	337-339	3	1

Chain B: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-28	22
α-helix	29-31	3
β-strand	34-40	7	8
α-helix	46-54	9
β-strand	69-76	8	8
β-strand	79-86	8	8
α-helix	95-98	4
β-strand	105-111	7	8
α-helix	118-130	13
β-strand	138-144	7	8
α-helix	146-155	10
α-helix	160-163	4
α-helix	172-173	2
α-helix	184-203	20
β-strand	211-212	2	8
α-helix	223-242	20

Chain C: 0 helices, 2 β-strands

Element	Residues	Length	Sheet
β-strand	5-7	3	14
β-strand	10-12	3	14

Chain D: 1 helix, 20 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	18-25	8	9
β-strand	33-39	7	10
β-strand	45-51	7	10
β-strand	58-60	3	10
β-strand	68-73	6	9
β-strand	78-83	6	9
β-strand	93-99	7	10
β-strand	111	1	10
β-strand	115-116	2	10
β-strand	128-130	3	11
β-strand	134-136	3	12
β-strand	143-149	7	11
β-strand	162-167	6	12
β-strand	174-178	5	12
β-strand	182-183	2	12
β-strand	192-196	5	11
β-strand	199-204	6	11
β-strand	214-219	6	12
α-helix	225	1
β-strand	231-234	4	12

Chain E: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	41-67	27
α-helix	68-72	5
α-helix	78-94	17
α-helix	96-105	10
α-helix	112-145	34
α-helix	150-153	4
α-helix	156-173	18
α-helix	175-179	5
β-strand	184-186	3	13
β-strand	190-192	3	13
α-helix	203-213	11
α-helix	214-218	5
α-helix	219-237	19
α-helix	251-281	31
α-helix	288-309	22
α-helix	310-314	5
α-helix	317-323	7

Chain F: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	12-23	12
α-helix	30-43	14
α-helix	53-55	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	A	protein	338	Homo sapiens	P62873 (AlphaFold model)
Engineered Guanine nucleotide-binding protein G(q) subunit alpha	B	protein	243	Homo sapiens
single Fab chain (svFv16)	D	protein	258	Homo sapiens
Somatostatin receptor type 2	E	protein	337	Homo sapiens	P30874 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	F	protein	71	Homo sapiens	P59768 (AlphaFold model)
somatostatin-14	C	protein	12	Homo sapiens	P61278 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7Y27_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains A)
ELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMH
WGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICS
IYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTG
HTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFA
TGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKA
DRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (B), FASTA

>7Y27_2 Engineered Guanine nucleotide-binding protein G(q) subunit alpha (chains B)
TVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGSGGS
GGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALN
DFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGED
PRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNLREY
NLV

Sequence of entity 3 (D), FASTA

>7Y27_3 single Fab chain (svFv16) (chains D)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSS
GGGSGGGGSGGGGSSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRP
GQSPQLLIYRMSNLASGVPERFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGA
GTKLELKAAAHHHHHHHH

Sequence of entity 4 (E), FASTA

>7Y27_4 Somatostatin receptor type 2 (chains E)
MDMADEPLNGSHTWLSIPFDLNGSVVSTNTSNQTEPYYDLTSNAVLTFIYFVVCIIGLCG
NTLVIYVILRYAKMKTITNIYILNLAIADELFMLGLPFLAMQVALVHWPFGKAICRVVMT
VDGINQFTSIFCLTVMSIDRYLAVVHPIKSAKWRRPRTAKMITMAVWGVSLLVILPIMIY
AGLRSNQWGRSSCTINWPGESGAWYTGFIIYTFILGFLVPLTIICLCYLFIIIKVKSSGI
RVGSSKRKKSEKKVTRMVSIVVAVFIFCWLPFYIFNVSSVSMAISPTPALKGMFDFVVVL
TYANSCANPILYAFLSDNFKKSFQNVLCLVKVSGTDD

Sequence of entity 5 (F), FASTA

>7Y27_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains F)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFSAIL

Sequence of entity 6 (C), FASTA

>7Y27_6 somatostatin-14 (chains C)
CKNFFWKTFTSC

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	1

Primary citation

Molecular basis for the selective G protein signaling of somatostatin receptors. Chen, S., Teng, X., Zheng, S. Nat Chem Biol (2023) 19:133-140. DOI 10.1038/s41589-022-01130-3 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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