8AE6: SEA complex wing

Cryo-EM structure of the SEA complex wing (SEACIT). Determined by electron microscopy at 2.7 Å resolution. Released 2 Nov 2022.

Method: Electron microscopy
Resolution: 2.7 Å
Organism: Saccharomyces cerevisiae
Chains: 4
Atoms: 19,788
Mol. weight: 513.39 kDa
Released: 2 Nov 2022

Explore 8AE6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8AE6 contains 109 α-helices and 141 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain Q: 11 helices, 37 β-strands

Element	Residues	Length	Sheet
β-strand	19-22	4	11
β-strand	29-32	4	12
β-strand	38-43	6	12
β-strand	46-51	6	12
α-helix	58-59	2
β-strand	60-63	4	12
α-helix	69-70	2
β-strand	73-75	3	13
β-strand	84-89	6	13
β-strand	92-97	6	13
β-strand	106-111	6	13
β-strand	117-122	6	14
β-strand	129-134	6	14
β-strand	139-143	5	14
β-strand	152-154	3	14
β-strand	161-166	6	15
β-strand	173-178	6	15
β-strand	181-186	6	15
β-strand	193-197	5	15
β-strand	204-209	6	16
β-strand	216-221	6	16
β-strand	225-230	6	16
β-strand	240-243	4	16
β-strand	248-253	6	17
β-strand	259-264	6	17
α-helix	268-270	3
β-strand	272-276	5	17
β-strand	288-290	3	16
β-strand	295-297	3	17
β-strand	304-312	9	11
β-strand	319	1	18
β-strand	323-330	8	11
β-strand	334-339	6	11
α-helix	342-347	6
β-strand	355	1	18
α-helix	359-361	3
α-helix	371-372	2
β-strand	391	1	10
α-helix	401-406	6
β-strand	411-412	2	11
α-helix	430-440	11
β-strand	445-450	6	19
β-strand	455-462	8	19
β-strand	473-480	8	19
α-helix	491-492	2
β-strand	493-496	4	19
α-helix	504-522	19
α-helix	530-536	7

Chain S: 25 helices, 18 β-strands

Element	Residues	Length	Sheet
α-helix	9-11	3
β-strand	13-20	8	1
β-strand	24-31	8	1
α-helix	35-38	4
α-helix	47-50	4
β-strand	61-65	5	1
β-strand	68-78	11	1
β-strand	82	1	35
α-helix	83-85	3
β-strand	86-96	11	1
α-helix	105-122	18
α-helix	124-127	4
β-strand	135	1	35
α-helix	197-210	14
β-strand	213-217	5	36
β-strand	223-227	5	36
α-helix	233-236	4
α-helix	243	1
β-strand	244-247	4	37
β-strand	249	1	28
α-helix	261-266	6
α-helix	267-269	3
α-helix	276-282	7
α-helix	287-299	13
β-strand	303-306	4	37
β-strand	314-317	4	38
α-helix	321-324	4
α-helix	330-338	9
β-strand	339	1	39
α-helix	346-348	3
α-helix	432-434	3
α-helix	435-443	9
α-helix	451-457	7
α-helix	460-464	5
β-strand	467	1	39
α-helix	469-478	10
β-strand	482-485	4	38
β-strand	487-491	5	40
α-helix	566-569	4
α-helix	570-581	12
α-helix	586-593	8
α-helix	597-607	11
β-strand	610-614	5	40

Chain U: 31 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	10-13	4	1
β-strand	16-18	3	2
β-strand	23-25	3	2
β-strand	29	1	1
α-helix	34-38	5
α-helix	365-376	12
α-helix	383-386	4
β-strand	396	1	3
β-strand	401	1	3
α-helix	402-408	7
β-strand	420	1	4
β-strand	422-424	3	1
β-strand	427-430	4	1
β-strand	431	1	4
β-strand	434-435	2	2
α-helix	526-529	4
β-strand	532-534	3	2
β-strand	537-539	3	1
α-helix	546-553	8
α-helix	554-558	5
α-helix	559-572	14
α-helix	574-590	17
α-helix	594-597	4
α-helix	602-616	15
α-helix	619-631	13
β-strand	636-640	5	5
β-strand	644-648	5	5
β-strand	655-656	2	6
α-helix	659-661	3
β-strand	672-673	2	6
α-helix	679-682	4
α-helix	744-746	3
β-strand	748-751	4	7
α-helix	755-762	8
α-helix	763-765	3
α-helix	771-781	11
α-helix	789-793	5
α-helix	794-797	4
α-helix	816-830	15
β-strand	834-837	4	7
α-helix	838-840	3
β-strand	845	1	8
β-strand	846-848	3	9
α-helix	890-901	12
α-helix	908-914	7
β-strand	922	1	8
α-helix	923-925	3
α-helix	933-945	13
β-strand	949-951	3	9
β-strand	953-960	8	10
α-helix	962-974	13
β-strand	1071-1073	3	10
α-helix	1081-1090	10
α-helix	1096-1104	9
β-strand	1110	1	10
α-helix	1115-1122	8
α-helix	1126-1135	10
β-strand	1140-1145	6	10

Chain W: 42 helices, 59 β-strands

Element	Residues	Length	Sheet
β-strand	102-104	3	20
β-strand	107-109	3	21
β-strand	118-119	2	21
β-strand	133-137	5	20
β-strand	151-155	5	20
α-helix	161-166	6
β-strand	173-175	3	21
α-helix	178-183	6
β-strand	191-195	5	20
α-helix	200-202	3
β-strand	203	1	22
β-strand	206-211	6	23
α-helix	218-227	10
β-strand	231-234	4	22
β-strand	238-239	2	23
β-strand	247-248	2	23
β-strand	252-254	3	23
β-strand	257-258	2	23
β-strand	261-264	4	22
β-strand	269-274	6	23
β-strand	278-285	8	24
α-helix	287-289	3
α-helix	291	1
β-strand	292	1	25
α-helix	293	1
β-strand	298	1	25
α-helix	299-302	4
α-helix	303-307	5
α-helix	308-318	11
β-strand	323-333	11	24
α-helix	339-341	3
α-helix	342-343	2
α-helix	346-348	3
β-strand	351-363	13	24
α-helix	364-366	3
α-helix	367-386	20
β-strand	387-389	3	26
α-helix	390	1
β-strand	395-397	3	26
β-strand	400-401	2	24
α-helix	402-403	2
α-helix	409-417	9
α-helix	418-420	3
β-strand	434-440	7	24
β-strand	445-447	3	27
α-helix	450-460	11
β-strand	467-471	5	24
α-helix	474-475	2
β-strand	482-486	5	27
β-strand	492-497	6	27
β-strand	500-504	5	24
α-helix	523-526	4
β-strand	536	1	28
α-helix	553-562	10
β-strand	848-851	4	27
α-helix	860-865	6
α-helix	887-890	4
α-helix	892	1
β-strand	893	1	23
α-helix	894-896	3
β-strand	898-899	2	29
α-helix	905-908	4
β-strand	913-919	7	29
α-helix	932-946	15
β-strand	949-951	3	29
α-helix	955-960	6
β-strand	986-989	4	29
β-strand	993-998	6	29
β-strand	1005-1011	7	29
β-strand	1028-1030	3	24
β-strand	1038-1039	2	24
α-helix	1050-1052	3
α-helix	1054-1062	9
β-strand	1076-1084	9	30
α-helix	1086-1088	3
α-helix	1091-1093	3
α-helix	1105-1121	17
β-strand	1124	1	30
β-strand	1147	1	31
α-helix	1152-1158	7
β-strand	1340-1342	3	32
β-strand	1344-1350	7	30
β-strand	1362-1368	7	30
β-strand	1371	1	31
β-strand	1375	1	33
β-strand	1377-1383	7	30
α-helix	1388-1404	17
β-strand	1407-1412	6	30
α-helix	1417-1422	6
β-strand	1430-1432	3	33
α-helix	1447-1452	6
α-helix	1456-1464	9
β-strand	1467-1469	3	33
β-strand	1485-1487	3	32
β-strand	1488	1	34
β-strand	1491	1	34
β-strand	1498-1502	5	33
β-strand	1507-1511	5	33
β-strand	1517-1521	5	33
α-helix	1551-1559	9
α-helix	1562-1577	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Nitrogen permease regulator 3	U	protein	1146	Saccharomyces cerevisiae	P38742 (AlphaFold model)
Maintenance of telomere capping protein 5	Q	protein	1148	Saccharomyces cerevisiae	Q03897 (AlphaFold model)
Vacuolar membrane-associated protein IML1	W	protein	1584	Saccharomyces cerevisiae	P47170 (AlphaFold model)
Nitrogen permease regulator 2	S	protein	615	Saccharomyces cerevisiae	P39923 (AlphaFold model)

Sequence of entity 1 (U), FASTA

>8AE6_1 Nitrogen permease regulator 3 (chains U)
MDECLPNSCLLGVHLVISTHSGPQIVYHYPPSNTAFLTNNPTKHQHLYGNHANLNKNTST
NKEEKLFNSGSTKTASQIALNESAKSYNTAITPSMTNTNTNNVTLPPTRSHANTVGSQSS
IPAATNGVGYRKTDIEDTSRTFQYQETESETSSSGLSDSELSTDYLDISSDSFSISSSLS
SSSLSSSPSSSSSSSPPQDGLSRTNSSFQSTDSMSPTSPQMIMENDSISVAESYLDSGTN
NKSRAASKRSQNFFHKLSTKKSTDSKTHSPVRKLKSKPSQSTKKGNKLLKNTSNETDGNA
FTGSCSISSKKSLSSTGEHNQELRNSSLNDTPGQSPHHYHHRYHHYHKNAATSQRNSHTQ
YDVEEEDMEVSAMLQDGKISMNEIFFEEENFQDINKILEFDNDFVAEFCSPEREMCNTRF
EFTVDNFCFLGLPIHVDSQGRWRKSKHKNKTRSKRSSSTTTNISRKKSIASKISSLSENT
LKKVNSGEADTVYDSNIGHEASTDTPNLRINTDVSGNEFEREKEDLGKNMNMFHVCFVMN
PHLIEYNKRIDDMYQFVVTRLSLLLRYVQSKTSYISSECHIILKEKERVLKHSKTYQSIR
GAGNKGKYLYQRILAKSSLARALTECVDKIQRNEIACLEINDDKVISLQIPIQNEFEKMP
NFKLQPVLRGSYLTSILNMKFLEKSSLRIESQNRQNDQAQFSDTNNNIYRFGNNINSTGH
CGAANVDDGDDNESNYYCDDNDDLLNYALLLLDEPNNIISSLETFSYQDDIGTIILKHLV
RNIQPNIPLRSYRYLITDLLDNPSSLDDLTTETNSLESSILRSCALHLMYWRHARIVIPL
SSKYTYIVSPLAPIQGYTIDDYKSTSQNDGNVKKMDDRENNKSGSDRVPLIYQNSMLFRS
KFPSLPSLPIFLSLLSTDKPQAYSNIIPSREHKPVYLNALAWLIQYGYVTQLLTFINIRV
DKHIKMAVDEDLEKEGFRKTNTARRPSMDYKKTDKKLDDEDGQSRDANASEACSGKNEGM
QSNDNNKDVDEKDNENDSRVDDRDDNEIAIADEEEILHFEYDDPEMQHDYTIILEPERAT
AIEKRWLYRCIYGQPSDIQILFNKLLKYFNGKVPMELVIIKEEISRHDLKKLLNALDKYL
IEIHHW

Sequence of entity 2 (Q), FASTA

>8AE6_2 Maintenance of telomere capping protein 5 (chains Q)
MCSSINEGPYNSPTFGKSLSLKVDGGFNAVSINPSGRDIVLASRQGLYIIDLDDPFTPPR
WLHHITPWQVADVQWSPHPAKPYWIVSTSNQKAIIWNLAKSSSNAIEFVLHGHSRAITDI
NFNPQHPDVLATCSVDTYVHAWDMRSPHRPFYSTSSWRSAASQVKWNYKDPNVLASSHGN
DIFVWDLRKGSTPLCSLKGHVSSVNSIDFNRFKYSEIMSSSNDGTVKFWDYSKSTTESKR
TVTTNFPIWRGRYLPFGEGYCIMPMVGGNNAVYLINLCDDDDSEQNKKTKLQPIYAFKGH
SDRVIDFLWRSRHTCDGDYDDREFQLVTWSKDCDLKLWPISDSIYGKVNFDRGKRLEEKL
PDYDYCSYNKEPENRENVQKNEFRRLRENFVTTSGLKKNKTNHITWLSGIRMNSATSQED
LFNETKIQNLGEEVSAIGHKFPKVVFEKISVSTRELCLTLNGPWSEENPDDYIFLRISIN
FPLNYPNKGDPPKFTIEENSNLTMSKRQEILSNLATIGQKYTDSNLYCLEPCIRFVLGEK
VSLEDIEEGQEPLLNFDIADHIDFEELSSLDSSYSDSQNPENLSSQSDIESYKEALVFPD
TSNQGLDFGRNLALDTTPVPNGCGSCWTATGELFCFFANEKKPEKKQNAIIKLSQKEAGV
EKHPFKIEPQVLYDKEVDSSVITAADELKARPKRYVDTLGLGGGTNGDSRTYFDDETSSD
DSFDSVADDWDDILRNDIIVRTKIPILRGNFKAFSSVHSESGKTVESTKKNKNLVISKNF
SSLLSDRKELALEYLFMDATPEGFARNNALVAEKFDLDEISHCWQILSDMLIDQSDYDPY
TTIWNNHPMGIKWFIKEAIVYFERQQNLQMLAMLCCVILSARRKKIPARYYGQELENMEG
TIVFNDNESQNTSFWKGSDAFSTRSRSSTVTPNFYGNHLRGKNIHGGDNSSIRSDDHHAR
LRTHNTLNGSSKFTEPAQKQGSRAISSSPFHSRMPDIKVELLHDDIIEAYEQEDLLHLEV
SDIPKFQTYIYQYSKLLFRWGLPLERVKILKVSTDFRSSYSSQGIPPNNNKKSPYNGVLT
HWIENNEFGEEKFLARNCNYCDLRVTRSSFICGNCQHVLHSSCARIWWEIGDECPSGCGC
NCPEMFDA

Sequence of entity 3 (W), FASTA

>8AE6_3 Vacuolar membrane-associated protein IML1 (chains W)
MFAKLHGKKQRPISSINSQTPRTSNTTHANSISLSSGNLIVGSNRNLRQKKEQFGSQQRA
SGRKLISNKENDDNVNNGGDNNYDNGERVHRHHIPGLKIKAYQAELGYHESRFSENLVML
NLVEFPDIKPGDLVELKTYHKNPSASNGDKKIYFIAKDFDGETKRRAKTSNVSILSGQLQ
TLLDLPSRSRIWIKLKPNKFDLQADVVEFNIKDCLLNRGDMWVLSSKLVDTCVFMDQRLA
FLDSIRGTIKGIYRNGKKIVSGYIGEQTRIIFRSESARLIFLIQITDEMWNFEETGEQLF
QKMVNSFFPKIFKKWKDVDTHHTITIAFAISMDLSDTSFKDLTPGESLKNSQDYFRIVVD
QVSIIHWVDIMETLREEFMEIRKDLLNKQTDKGYSVANGRFSPVIKSNFLELVNFATTIL
TDPFKQLDLRHTTTHVMIISPGSGLFDVDYSLLRLTGKKLLSLEMTMDLICLSKAPLHIV
PLFRYRDFENKLHHCVPLWLSVFFWNDHDKKSNSEWTPRCKIYDLQMMGITENELIREVD
VEYLQLNKKVKSLSEFMNDYDKNAFEVKILCAGSNTKQSKKLNSKFDTVFENDVVVKARK
IPATATTTHGNTKFIWRGPKVALPAIKDIQKPNVIPDLSIKTIEASFYDDCNTTNDKIST
PTTSNNDNLEMNDSLVSVRSADNQNTSLALDSLKGLSKRNSLKDFTQRVITKFISNIDTS
KNKKIKSTLLRDDVDNSPLGSNTPLPSSESKISGLKLQQKGLADENVISKRGNLIIKKNL
SIFGLPSNEIMSGSPSSYLGSSHTRTSSKLSNMSDKAAFITEGQKSKHDDSNTYSLTQQL
KHRISETWVDIKSPSIPVSSEFANELLPIRWKDVWPKYVARKYSKWRSFTTPAELPITIS
DFPSKDDFDRNFIFRNHSVTLNTDQEQYNQTYKDLLRDMIYMRLLTGFQICVGRQVEKIE
LSRESGESETVVNKYLDFNQNDAFKLYLMIDSEIHRITCSSSGIIDVERYLRKDEANLFD
QVPSYIPLVKTRYESSFRDAMIDPLHVKRESLNWNQIDQVLAGYGDNLIDRKWHGFRAKY
VVLPTDIPPNTYSMVINGKSETLNPEEIRVEGLRRLIGSITRSRLRTEKEKKGRKTKREE
IQPEVMFYTGPLYNFINEQQTSLESSAINFKDSIFVNDNNLLNRNVELSKLAYQIQRGED
RITLVNRKWHWKKHEKCFVGSEMVNWLIRNFSDIDTREDAIKYGQKVMKEGLFVHVLNKH
NFLDGHYFYQFSPEYVMDTNKLEKTNSHRSTLSDPKQMLRKASTGSSNDPSAMTPFSSVV
PAISASNASVADAKEPSRPILMLSNSLVIDVDPAGKSSKQESCTVHYDRVHNPDHCFHIR
LEWLTTTPKLIDDLVGNWSRLCERYGLKMIEIPWEELCTIPSVNPFHSFVEIKLAINPWE
DPEFKDRELFAKSKFYYHVYLLKASGFLLDNRASKFLQNQDIEFDIMYSWGKPQFKYVQY
IHHTGAYVAELRENGCLFLAPNNIYISRVNPGNIIGKIHSASSSSLDAQKVILNFKSTCL
DYQKLRSIFLDAKEMWITGKIVED

Sequence of entity 4 (S), FASTA

>8AE6_4 Nitrogen permease regulator 2 (chains S)
MLSYFQGFVPIHTIFYSVFHPTEGSKIKYEFPPNNLKNHGINFNTFKNYIIPKPILCHKL
ITFKYGTYRIVCYPVTINSPIYARNFFSFNFVFVFPYDCETSPYEPAITRLGKMFKVLEE
QNQLLSKSERDPVFFDLKVLENSTTTPSTAGPSSTPNPSSNTTPTHPTSEKDTKDMRSSR
YSDLIKDLGLPQSAFSIQDLLMRIFQDLNNYSECLIPIDEGNAVDIKIFPLLRPPTTCVS
LEDVPLSSVNLKKIIDVNWDPTMMSIVPYIDGLNSIAKISKLSNSDPGLVIECIRHLIYY
KCVTLSDIFQFSNIYAPSSLIRNFLTDPLMASDCQSYVTFPEVSKISNLPLNKSLGSGDQ
DSPSFSVRRKSKSSSIPSNPDSRTTSFSSTSRVSQNSSLNSSFSSIYKDWRQSQTSCSSS
NIHVINNRNRFLPTRSCLFDLYRSLSQGQTLKTWYESKYMILKENNIDIRRFITFGLEKR
IIYRCYSFPVMINAGSREPKEMTPIITKDLVNNDKLLEKRNHNHLLSATGSRNTAQSGNL
KPERPSKVSFEMQRVSSLATGKSTMPKLSDEEEGILEESIRNAETFDKICVLLSKPKLEV
ESYLNELGEFKVINS

Primary citation

Cryo-EM structure of the SEA complex. Tafur, L., Hinterndorfer, K., Gabus, C. et al. Nature (2022) 611:399-404. DOI 10.1038/s41586-022-05370-0 · PubMed

Other PDB entries of the same protein (UniProt P38742 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8ADL 2.95 Å, Cryo-EM structure of the SEA complex
9H4Q 3.1 Å, Cryo-EM structure of the SEAC wing - EGOC
9H5K 3.2 Å, Cryo-EM structure of the SEAC-EGOC supercomplex

Browse structure collections

About this viewer

MolViewer shows 8AE6 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.