8B9D: Human replisome bound by Pol Alpha

Name: PDB 8B9D: Human replisome bound by Pol Alpha
Published: 2023-08-09
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: replication

Human replisome bound by Pol Alpha. Determined by electron microscopy at 3.4 Å resolution. Released 9 Aug 2023.

Method: Electron microscopy
Resolution: 3.4 Å
Organism: Homo sapiens
Chains: 23
Atoms: 66,159
Mol. weight: 1834.18 kDa
Ligands: ANP, MG, ZN
Released: 9 Aug 2023

Explore 8B9D in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8B9D contains 414 α-helices and 359 β-strands across 21 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 33 helices, 26 β-strands

Element	Residues	Length	Sheet
α-helix	182-187	6
α-helix	189-205	17
β-strand	207	1	1
β-strand	213	1	1
α-helix	214-224	11
β-strand	230-233	4	2
α-helix	234-247	14
α-helix	252-270	19
α-helix	274-276	3
β-strand	283-286	4	2
β-strand	291	1	3
α-helix	299-301	3
β-strand	305-315	11	3
α-helix	316-318	3
β-strand	319-322	4	3
β-strand	370-379	10	3
α-helix	380-381	2
α-helix	386	1
β-strand	393-399	7	3
α-helix	400-402	3
β-strand	411-421	11	3
α-helix	424-430	7
β-strand	434	1	4
β-strand	436-446	11	3
α-helix	458-467	10
α-helix	473-478	6
α-helix	489-500	12
β-strand	505	1	5
β-strand	513	1	5
β-strand	519-523	5	6
α-helix	529-539	11
β-strand	543-547	5	6
α-helix	548-550	3
β-strand	551	1	7
α-helix	553-556	4
β-strand	558-562	5	8
β-strand	569-573	5	8
α-helix	575-579	5
β-strand	583-587	5	6
α-helix	589-591	3
α-helix	594-597	4
α-helix	598-600	3
α-helix	601-605	5
β-strand	608-613	6	9
β-strand	616-621	6	9
β-strand	625-630	6	6
α-helix	633-635	3
α-helix	643-647	5
α-helix	651-654	4
β-strand	659-663	5	6
α-helix	669-685	17
α-helix	712-716	5
α-helix	717-730	14
β-strand	734	1	10
α-helix	740-757	18
α-helix	765-780	16
β-strand	785	1	10
α-helix	788-803	16
α-helix	808-817	10

Chain 3: 30 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	12-23	12
α-helix	25-27	3
α-helix	31-41	11
β-strand	46-50	5	11
α-helix	51-57	7
α-helix	59-67	9
α-helix	69-87	19
α-helix	89-92	4
β-strand	99-103	5	11
α-helix	107-109	3
β-strand	110-111	2	12
α-helix	118-120	3
β-strand	124-134	11	12
α-helix	135-137	3
β-strand	138-148	11	12
β-strand	153-158	6	12
β-strand	185-186	2	12
β-strand	192-202	11	12
α-helix	205-207	3
α-helix	208-209	2
α-helix	213-215	3
β-strand	216-222	7	12
α-helix	223-225	3
β-strand	234-244	11	12
β-strand	252	1	13
β-strand	255-256	2	14
β-strand	257-267	11	12
α-helix	282-289	8
α-helix	295-301	7
α-helix	311-322	12
β-strand	327-328	2	15
β-strand	334-335	2	15
β-strand	341-345	5	16
α-helix	351-358	8
β-strand	365-369	5	16
β-strand	373	1	17
α-helix	375-379	5
β-strand	380-384	5	18
β-strand	391-395	5	18
α-helix	397-400	4
β-strand	404-409	6	16
α-helix	411-413	3
α-helix	416-428	13
β-strand	430-435	6	19
β-strand	438-443	6	19
β-strand	446-452	7	16
α-helix	465-468	4
α-helix	473-476	4
β-strand	481-485	5	16
α-helix	491-505	15
α-helix	565-575	11
β-strand	581	1	20
α-helix	584-603	20
α-helix	606-608	3
α-helix	615-632	18
β-strand	636	1	20
α-helix	639-653	15

Chain 4: 32 helices, 35 β-strands

Element	Residues	Length	Sheet
α-helix	147-149	3
β-strand	150-152	3	21
β-strand	158	1	21
α-helix	159-172	14
α-helix	192-203	12
β-strand	207-211	5	22
α-helix	212-218	7
α-helix	220-228	9
α-helix	230-247	18
α-helix	257-258	2
β-strand	259-263	5	22
α-helix	276-278	3
β-strand	282-292	11	23
α-helix	293-295	3
β-strand	296-306	11	23
β-strand	312-316	5	23
β-strand	318	1	24
β-strand	321	1	24
β-strand	327	1	25
β-strand	334	1	25
β-strand	337-339	3	23
α-helix	341-343	3
β-strand	345-355	11	23
α-helix	358-360	3
α-helix	362	1
α-helix	365-368	4
β-strand	369-375	7	23
α-helix	376-378	3
β-strand	387-397	11	23
β-strand	400	1	26
β-strand	408-409	2	26
β-strand	411	1	27
β-strand	413-423	11	23
α-helix	443-454	12
α-helix	458-465	8
α-helix	474-485	12
β-strand	506-510	5	28
α-helix	516-524	9
β-strand	531-534	4	28
β-strand	546-549	4	29
β-strand	556-559	4	29
α-helix	562-565	4
β-strand	570-574	5	28
α-helix	576-578	3
α-helix	581-593	13
β-strand	595-600	6	30
β-strand	603-608	6	30
β-strand	612-616	5	28
α-helix	630-633	4
α-helix	638-641	4
β-strand	646-650	5	28
α-helix	656-670	15
α-helix	674-697	24
β-strand	701	1	31
α-helix	704-722	19
α-helix	731-747	17
β-strand	752	1	31
α-helix	755-773	19
β-strand	774-775	2	32
β-strand	780-781	2	32
α-helix	783-788	6
α-helix	792-811	20
β-strand	817-819	3	33
α-helix	820-830	11
α-helix	837-849	13
β-strand	853-856	4	33
β-strand	859-862	4	33

Chain 5: 32 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	22-41	20
β-strand	44-46	3	34
β-strand	50-52	3	34
α-helix	54-64	11
β-strand	69-73	5	35
α-helix	74-80	7
α-helix	82-90	9
α-helix	92-111	20
α-helix	112	1
α-helix	114	1
α-helix	121-123	3
β-strand	125-129	5	35
α-helix	137-139	3
α-helix	142-144	3
β-strand	148-158	11	14
α-helix	159-161	3
β-strand	162-172	11	14
β-strand	178-183	6	14
β-strand	191	1	13
β-strand	212-214	3	14
α-helix	216-218	3
β-strand	220-230	11	14
α-helix	241-243	3
β-strand	244-250	7	14
α-helix	251-253	3
β-strand	262-271	10	14
β-strand	289	1	3
β-strand	292-301	10	14
α-helix	313-315	3
α-helix	316-327	12
α-helix	331-338	8
α-helix	347-358	12
β-strand	363-364	2	36
β-strand	370-371	2	36
β-strand	377-381	5	37
α-helix	387-397	11
β-strand	401-405	5	37
β-strand	409	1	9
α-helix	411-414	4
β-strand	416-420	5	38
β-strand	427-431	5	38
α-helix	433-436	4
β-strand	441-445	5	37
α-helix	447-449	3
α-helix	452-455	4
α-helix	456-458	3
α-helix	459-463	5
β-strand	466-471	6	17
β-strand	474-479	6	17
β-strand	483-488	6	37
α-helix	500-503	4
α-helix	508-511	4
β-strand	516-520	5	37
α-helix	526-545	20
α-helix	556-569	14
β-strand	573-574	2	39
α-helix	576-599	24
α-helix	610-626	17
β-strand	631-632	2	39
α-helix	634-651	18

Chain 6: 28 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	21-36	16
β-strand	38	1	40
β-strand	44	1	40
α-helix	45-50	6
α-helix	51-54	4
β-strand	60-64	5	41
α-helix	65-71	7
α-helix	73-101	29
β-strand	111-115	5	41
α-helix	123-125	3
α-helix	128-130	3
β-strand	131	1	4
β-strand	134-144	11	4
β-strand	148-158	11	4
β-strand	163-169	7	4
α-helix	183-185	3
β-strand	191-193	3	4
β-strand	199-209	11	4
α-helix	219-222	4
β-strand	223-229	7	4
α-helix	230-232	3
β-strand	241-252	12	4
α-helix	254-256	3
β-strand	281	1	42
β-strand	282	1	24
α-helix	285-287	3
β-strand	292	1	42
β-strand	294-305	12	4
α-helix	323-328	6
α-helix	331-341	11
α-helix	346-354	9
α-helix	362-371	10
β-strand	378-379	2	43
β-strand	385-386	2	43
β-strand	392-396	5	44
α-helix	402-412	11
β-strand	416-420	5	44
β-strand	432-436	5	45
β-strand	441-445	5	45
α-helix	448-451	4
β-strand	455-460	6	44
α-helix	467-479	13
β-strand	481-486	6	7
β-strand	489-494	6	7
β-strand	497-503	7	44
α-helix	516-519	4
α-helix	524-527	4
β-strand	532-536	5	44
α-helix	542-557	16
α-helix	569-579	11
α-helix	584	1
β-strand	585-586	2	46
α-helix	587	1
α-helix	588-607	20
α-helix	618-634	17
β-strand	639-640	2	46
α-helix	642-660	19

Chain 7: 33 helices, 26 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
α-helix	24-27	4
α-helix	32-41	10
β-strand	47-51	5	47
α-helix	52-58	7
α-helix	60-67	8
α-helix	70-88	19
α-helix	92-95	4
α-helix	98-110	13
α-helix	128-131	4
β-strand	135-139	5	47
α-helix	140-141	2
α-helix	145-146	2
β-strand	147	1	27
α-helix	148	1
α-helix	154-156	3
β-strand	160-170	11	27
α-helix	171-173	3
β-strand	174-184	11	27
β-strand	190-194	5	27
β-strand	199-200	2	26
β-strand	205	1	48
α-helix	209-214	6
β-strand	219	1	48
β-strand	220-222	3	27
β-strand	228-238	11	27
α-helix	241-243	3
α-helix	245	1
β-strand	252-258	7	27
α-helix	259-261	3
β-strand	270-279	10	27
α-helix	280-281	2
β-strand	293	1	12
β-strand	296-305	10	27
α-helix	332-339	8
α-helix	348-359	12
β-strand	377-381	5	49
α-helix	387-397	11
β-strand	401-405	5	49
β-strand	417-418	2	50
β-strand	429-430	2	50
α-helix	433-436	4
β-strand	441-445	5	49
α-helix	447-449	3
α-helix	452-464	13
β-strand	466-468	3	51
β-strand	477-479	3	51
β-strand	483-488	6	49
α-helix	501-505	5
α-helix	509-512	4
β-strand	517-521	5	49
α-helix	527-542	16
α-helix	556-566	11
β-strand	572	1	52
α-helix	575-594	20
α-helix	603-619	17
β-strand	624	1	52
α-helix	627-640	14
α-helix	641-644	4

Chain A: 18 helices, 33 β-strands

Element	Residues	Length	Sheet
α-helix	97-111	15
β-strand	172-174	3	53
β-strand	193-196	4	54
β-strand	208	1	55
α-helix	213-235	23
β-strand	247-259	13	56
β-strand	270-274	5	56
β-strand	283-287	5	56
β-strand	295-296	2	57
β-strand	301-308	8	56
β-strand	314-320	7	56
α-helix	321-322	2
α-helix	324-326	3
β-strand	327	1	58
α-helix	328	1
β-strand	340-346	7	53
α-helix	358-370	13
β-strand	374-378	5	53
α-helix	380	1
β-strand	383	1	59
α-helix	387-390	4
α-helix	398-413	16
α-helix	414-416	3
β-strand	419-423	5	53
β-strand	428	1	59
β-strand	435	1	60
β-strand	437	1	61
α-helix	438-440	3
α-helix	447-452	6
β-strand	453-455	3	53
β-strand	458	1	61
β-strand	460-464	5	54
β-strand	467-471	5	54
α-helix	476-481	6
β-strand	485-486	2	57
α-helix	494-504	11
β-strand	507	1	62
α-helix	514-515	2
β-strand	520	1	60
α-helix	522-528	7
β-strand	530	1	62
β-strand	534	1	58
β-strand	537-539	3	54
α-helix	545-546	2
β-strand	547-551	5	54
β-strand	554-558	5	54
β-strand	563-564	2	63
β-strand	567-568	2	63
β-strand	570-576	7	53
α-helix	578-580	3
β-strand	590-597	8	53

Chain B: 9 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	1280-1282	3	92
β-strand	1289-1291	3	92
β-strand	1296-1297	2	93
α-helix	1300-1302	3
β-strand	1304-1305	2	93
α-helix	1319-1321	3
α-helix	1323-1342	20
β-strand	1346-1348	3	94
β-strand	1356-1357	2	94
β-strand	1364-1365	2	95
β-strand	1368-1369	2	95
β-strand	1370	1	96
α-helix	1371	1
β-strand	1378	1	96
β-strand	1379-1381	3	94
α-helix	1385-1399	15
α-helix	1401-1406	6
α-helix	1411-1420	10
α-helix	1424-1443	20
β-strand	1444	1	55
β-strand	1449-1450	2	80
α-helix	1452-1456	5

13 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2	protein	904	Homo sapiens	P49736 (AlphaFold model)
DNA replication licensing factor MCM3	3	protein	808	Homo sapiens	P25205 (AlphaFold model)
DNA replication licensing factor MCM4	4	protein	863	Homo sapiens	P33991 (AlphaFold model)
DNA replication licensing factor MCM5	5	protein	734	Homo sapiens	P33992 (AlphaFold model)
DNA replication licensing factor MCM6	6	protein	821	Homo sapiens	Q14566
DNA replication licensing factor MCM7	7	protein	719	Homo sapiens	P33993
DNA polymerase alpha subunit B	A	protein	598	Homo sapiens	Q14181
Cell division control protein 45 homolog	C	protein	569	Homo sapiens	O75419
DNA replication complex GINS protein PSF1	D	protein	196	Homo sapiens	Q14691
DNA replication complex GINS protein PSF2	E	protein	185	Homo sapiens	Q9Y248
DNA replication complex GINS protein PSF3	F	protein	216	Homo sapiens	Q9BRX5
DNA replication complex GINS protein SLD5	G	protein	261	Homo sapiens	Q9BRT9

9 more molecules are not listed.

Sequence of entity 1 (2), FASTA

>8B9D_1 DNA replication licensing factor MCM2 (chains 2)
MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTE
GPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQR
DREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGH
SVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAR
EHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQL
HLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGP
FEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHN
NYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFAS
IAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVS
SRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSI
HEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDIL
CVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVL
KKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARI
HLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLV
AEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMI
LQQF

Sequence of entity 2 (3), FASTA

>8B9D_2 DNA replication licensing factor MCM3 (chains 3)
MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEK
RANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCF
LSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEE
NNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGT
YRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLA
KSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLC
TAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRT
AIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFD
LLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQ
DTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQ
DSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEK
EKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQV
HTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPF
SSVEIQAALSKMQDDNQVMVSEGIIFLI

Sequence of entity 3 (4), FASTA

>8B9D_3 DNA replication licensing factor MCM4 (chains 4)
MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQ
SPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGS
AQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKE
EENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTFDMA
VNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIPEMQ
EAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQESPED
MPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIH
YRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGI
LLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSA
VGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAK
AGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLLLDPQDEAYDR
RLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKI
GSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGI
VDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMF
EEALRALADDDFLTVTGKTVRLL

Sequence of entity 4 (5), FASTA

>8B9D_4 DNA replication licensing factor MCM5 (chains 5)
MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELK
RHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEV
LQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLT
NIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGE
MPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQ
VDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGG
SRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMR
DPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNS
RCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLH
VSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDS
DRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGV
EGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGE
IQHRMQRKVLYRLK

Sequence of entity 5 (6), FASTA

>8B9D_5 DNA replication licensing factor MCM6 (chains 6)
MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNT
LVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTR
HKIRELTSSRIGLLTRISGQVVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNIC
RNPVCANRRRFLLDTNKSRFVDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGD
KCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLA
CCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHG
NDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTS
GKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQ
TISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDEC
NEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKH
LRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVE
TPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEY
CRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRL
THYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED

Sequence of entity 6 (7), FASTA

>8B9D_6 DNA replication licensing factor MCM7 (chains 7)
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDP
ELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVR
SPQNQYPAELMRRFELYFQGPSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVA
TYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEH
SDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEA
HRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDVKKALLLLLVG
GVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR
DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNA
RCSILAAANPAYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYV
HQHSRQPPSQFEPLDMKLMRRYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATY
TSARTLLAILRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPADV
IFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV

Sequence of entity 7 (A), FASTA

>8B9D_7 DNA polymerase alpha subunit B (chains A)
MSASAQQLAEELQIFGLDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSE
ILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGS
QKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGV
SWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAF
TPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGDREHSSGAQIPVDLSELKEYSLFPGQ
VVIMEGINTTGRKLVATKLYEGVPLPFYQPTEEDADFEQSMVLVACGPYTTSDSITYDPL
LDLIAVINHDRPDVCILFGPFLDAKHEQVENCLLTSPFEDIFKQCLRTIIEGTRSSGSHL
VFVPSLRDVHHEPVYPQPPFSYSDLSREDKKQVQFVSEPCSLSINGVIFGLTSTDLLFHL
GAEEISSSSGTSDRFSRILKHILTQRSYYPLYPPQEDMAIDYESFYVYAQLPVTPDVLII
PSELRYFVKDVLGCVCVNPGRLTKGQVGGTFARLYLRRPAADGAERQSPCIAVQVVRI

Sequence of entity 8 (C), FASTA

>8B9D_8 Cell division control protein 45 homolog (chains C)
MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELET
AFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLIK
QDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRLDYKDDDIVEQTMRRRQRREW
EARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLWWAIVGLTDQWVQDKITQMK
YVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTA
ARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMR
VQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLE
LAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSF
VCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRM
LHNHFDLSVIELKAEDRSKFLDALISLLS

Sequence of entity 9 (D), FASTA

>8B9D_9 DNA replication complex GINS protein PSF1 (chains D)
MFCEKAMELIRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSGGRSDLIP
TIKFRHCSLLRNRRCTVAYLYDRLLRIRALRWEYGSVLPNALRFHMAAEEMEWFNNYKRS
LATYMRSLGGDEGLDITQDMKPPKSLYIEVRCLKDYGEFEVDDGTSVLLKKNSQHFLPRW
KCEQLIRQGVLEHILS

Sequence of entity 10 (E), FASTA

>8B9D_10 DNA replication complex GINS protein PSF2 (chains E)
MDAAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGLPVEVPLWLAINLKQRQKCRL
LPPEWMDVEKLEKMRDHERKEETFTPMPSPYYMELTKLLLNHASDNIPKADEIRTLVKDM
WDTRIAKLRVSADSFVRQQEAHAKLDNLTLMEINTSGTFLTQALNHMYKLRTNLQPLEST
QSQDF

Sequence of entity 11 (F), FASTA

>8B9D_11 DNA replication complex GINS protein PSF3 (chains F)
MSEAYFRVESGALGPEENFLSLDDILMSHEKLPVRTETAMPRLGAFFLERSAGAETDNAV
PQGSKLELPLWLAKGLFDNKRRILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFG
SQLLHFDSPENADISQSLLQTFIGRFRRIMDSSQNAYNEDTSALVARLDEMERGLFQTGQ
KGLNDFQCWEKGQASQITASNLVQNYKKRKFTDMED

Sequence of entity 12 (G), FASTA

>8B9D_12 DNA replication complex GINS protein SLD5 (chains G)
WSHPQFEKGGGSGGGSGGSAWSHPQFEKSGLEVLFQGPMTEEVDFLGQDSDGGSEEVVLT
PAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQMEM
ERIRYVLSSYLRCRLMKIEKFFPHVLEKEKTRPEGEPSSLSPEELAFAREFMANTESYLK
NVALKHMPPNLQKVDLFRAVPKPDLDSYVFLRVRERQENILVEPDTDEQRDYVIDLEKGS
QHLIRYKTIAPLVASGAVQLI

Ligands and cofactors

ID	Name	Formula	Copies
ANP	Phosphoaminophosphonic acid-adenylate ester	C10 H17 N6 O12 P3	3
MG	Magnesium ion	Mg	3
ZN	Zinc ion	Zn	4

Primary citation

How Pol alpha-primase is targeted to replisomes to prime eukaryotic DNA replication. Jones, M.L., Aria, V., Baris, Y. et al. Mol Cell (2023) 83:2911. DOI 10.1016/j.molcel.2023.06.035 · PubMed

Other PDB entries of the same protein (UniProt P49736 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6YA7 1.67 Å, Cdc7-Dbf4 bound to an Mcm2-S40 derived bivalent substrate
7CIZ 1.8 Å, Crystal structure of DNAJC9 HBD helix2 in complex with H3.3-H4 dimer and MCM2 HBD
5BNX 2.31 Å, Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.3-H4 dimer
5JA4 2.42 Å, Crystal structure of human TONSL and MCM2 HBDs binding to a histone H3-H4 tetramer
7CJ0 2.5 Å, Crystal structure of DNAJC9 HBD in complex with H3.3-H4 dimer and MCM2 HBD
7W1Y 2.59 Å, Human MCM double hexamer bound to natural DNA duplex (polyAT/polyTA)
9E2Z 2.6 Å, Cryo-EM structure of human CMG helicase stalled at G4-containing DNA template
5BNV 2.79 Å, Crystal structure of Human MCM2 HBD chaperoning a histone H3-H4 tetramer
7PLO 2.8 Å, H. sapiens replisome-CUL2/LRR1 complex
8W0F 2.8 Å, Cryo-EM structure of a human MCM2-7 double hexamer on dsDNA
4UUZ 2.9 Å, MCM2-histone complex
5BO0 2.91 Å, Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.2-H4 dimer

Browse structure collections

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