Structure of CEACAM5 A3-B3 domain in Complex with Tusamitamab Fab. Determined by electron microscopy at 3.11 Å resolution. Released 24 Jan 2024.
Explore 8BW0 in 3D Show helices and sheets RCSB PDB PDBe
8BW0 contains 8 α-helices and 44 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 501-503 | 3 | |
| β-strand | 505-507 | 3 | 7 |
| β-strand | 513 | 1 | 8 |
| β-strand | 519-523 | 5 | 7 |
| β-strand | 531-536 | 6 | 9 |
| β-strand | 539-540 | 2 | 9 |
| β-strand | 547-550 | 4 | 7 |
| β-strand | 555-558 | 4 | 7 |
| α-helix | 563-565 | 3 | |
| β-strand | 569-574 | 6 | 9 |
| β-strand | 580-581 | 2 | 9 |
| α-helix | 582-584 | 3 | |
| β-strand | 585 | 1 | 9 |
| β-strand | 590 | 1 | 8 |
| α-helix | 591-593 | 3 | |
| β-strand | 597-598 | 2 | 10 |
| β-strand | 604-605 | 2 | 11 |
| β-strand | 611-614 | 4 | 12 |
| β-strand | 615-616 | 2 | 10 |
| α-helix | 622-623 | 2 | |
| β-strand | 624-629 | 6 | 13 |
| β-strand | 632-635 | 4 | 13 |
| β-strand | 639-642 | 4 | 12 |
| α-helix | 645-646 | 2 | |
| β-strand | 651-658 | 8 | 13 |
| β-strand | 665-672 | 8 | 13 |
| β-strand | 673-674 | 2 | 11 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 3-7 | 5 | 1 |
| β-strand | 17-25 | 9 | 1 |
| β-strand | 34-40 | 7 | 2 |
| β-strand | 44-51 | 8 | 2 |
| β-strand | 68-73 | 6 | 1 |
| β-strand | 78-84 | 7 | 1 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-99 | 8 | 2 |
| β-strand | 107-108 | 2 | 2 |
| β-strand | 114-116 | 3 | 2 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 4-6 | 3 | 3 |
| β-strand | 10 | 1 | 4 |
| β-strand | 19-29 | 11 | 3 |
| β-strand | 34-37 | 4 | 5 |
| β-strand | 45-47 | 3 | 5 |
| β-strand | 49 | 1 | 6 |
| β-strand | 53 | 1 | 6 |
| β-strand | 62-65 | 4 | 3 |
| β-strand | 68-75 | 8 | 3 |
| β-strand | 86-89 | 4 | 5 |
| α-helix | 96 | 1 | |
| β-strand | 97 | 1 | 2 |
| β-strand | 98 | 1 | 5 |
| β-strand | 102 | 1 | 5 |
| β-strand | 103 | 1 | 4 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Tusamitamab Fab heavy Chain | H | protein | 234 | Mus sp. | |
| Tusamitamab Light Chain | L | protein | 214 | Mus sp. | |
| Carcinoembryonic antigen-related cell adhesion molecule 5 | C | protein | 193 | Homo sapiens | P06731 (AlphaFold model) |
>8BW0_1 Tusamitamab Fab heavy Chain (chains H) EVQLQESGPGLVKPGGSLSLSCAASGFVFSSYDMSWVRQTPERGLEWVAYISSGGGITYA PSTVKGRFTVSRDNAKNTLYLQMNSLTSEDTAVYYCAAHYFGSSGPFAYWGQGTLVTVSS ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS GLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTHHHHHH
>8BW0_2 Tusamitamab Light Chain (chains L) DIQMTQSPASLSASVGDRVTITCRASENIFSYLAWYQQKPGKSPKLLVYNTRTLAEGVPS RFSGSGSGTDFSLTISSLQPEDFATYYCQHHYGTPFTFGSGTKLEIKRTVAAPSVFIFPP SDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLT LSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
>8BW0_3 Carcinoembryonic antigen-related cell adhesion molecule 5 (chains C) ELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTL FNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSA SNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASG TSPGLSAHHHHHH
| ID | Name | Formula | Copies |
|---|---|---|---|
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 3 |
Structural insights into epitope-paratope interactions of a monoclonal antibody targeting CEACAM5-expressing tumors. Kumar, A., Duffieux, F., Gagnaire, M. et al. Nat Commun (2024) 15:9377-9377. DOI 10.1038/s41467-024-53746-9 · PubMed
Other PDB entries of the same protein (UniProt P06731 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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