8C7Y: BRAF V600E

Crystal structure of BRAF V600E in complex with a hybrid compound 6. Determined by X-ray diffraction at 1.65 Å resolution. Released 22 Feb 2023.

Method: X-ray diffraction
Resolution: 1.65 Å
Organism: Homo sapiens
Chains: 2
Atoms: 4,948
Mol. weight: 66.45 kDa
Ligands: TXV
Released: 22 Feb 2023

Explore 8C7Y in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8C7Y contains 33 α-helices and 20 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	451	1	1
α-helix	452-453	2
β-strand	458-466	9	1
β-strand	469-475	7	1
β-strand	479-484	6	1
α-helix	492-505	14
β-strand	513	1	2
β-strand	516-520	5	1
β-strand	526-530	5	1
β-strand	534-536	3	2
α-helix	537-538	2
α-helix	539-543	5
α-helix	550-569	20
α-helix	579-581	3
β-strand	582-585	4	2
β-strand	589-592	4	2
α-helix	609-612	4
α-helix	614-619	6
α-helix	622-626	5
α-helix	635-651	17
α-helix	662-671	10
α-helix	678-680	3
α-helix	687-696	10
α-helix	701-703	3
α-helix	705-706	2
α-helix	707-721	15

Chain B: 17 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	451	1	3
α-helix	452-453	2
β-strand	458-465	8	3
β-strand	470-475	6	3
β-strand	479-484	6	3
α-helix	492-505	14
β-strand	513	1	4
β-strand	516-520	5	3
β-strand	526-530	5	3
α-helix	531-533	3
β-strand	534-536	3	4
α-helix	537-538	2
α-helix	539-543	5
α-helix	550-569	20
α-helix	579-581	3
β-strand	582-585	4	4
β-strand	589-592	4	4
α-helix	596-598	3
α-helix	617-619	3
α-helix	622-625	4
α-helix	635-651	17
α-helix	662-671	10
α-helix	678-680	3
α-helix	687-696	10
α-helix	701-703	3
α-helix	705-706	2
α-helix	707-721	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Serine/threonine-protein kinase B-raf	A, B	protein	282	Homo sapiens	P15056 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>8C7Y_1 Serine/threonine-protein kinase B-raf (chains A, B)
SMRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNE
VGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHASETKFEMKKLIDIARQTA
RGMDYLHAKSIIHRDLKSNNIFLHEDNTVKIGDFGLATEKSRWSGSHQFEQLSGSILWMA
PEVIRMQDSNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIEMVGRGSLSPDLSKV
RSNCPKRMKRLMAECLKKKRDERPSFPRILAEIEELARELSG

Ligands and cofactors

ID	Name	Formula	Copies
TXV	~{N}-[3-[(5-chloranyl-1~{H}-pyrrolo[2,3-b]pyridin-3-yl)carbonyl]-2,4-bis(fluora…	C25 H17 Cl F2 N4 O2	2

Water and common crystallization additives (NO3, EDO) are not listed.

Primary citation

Design, synthesis and characterisation of a novel type II B-RAF paradox breaker inhibitor. Arora, R., Linders, J.T.M., Aci-Seche, S. et al. Eur J Med Chem (2023) 250:115231-115231. DOI 10.1016/j.ejmech.2023.115231 · PubMed

Other PDB entries of the same protein (UniProt P15056 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8VSO 1.5 Å, Ternary structure of 14-3-3 sigma, BRAF phosphopeptide (pS365) and compound 78 (1124378)
8JNB 1.62 Å, CRAF ras-binding domain chimera, ligand complex
8C7X 1.65 Å, Crystal structure of BRAF in complex with a hybrid compound 6
9EW6 1.65 Å, Binary structure of 14-3-3s and BRAF phosphopeptide (pS365)
8JNA 1.7 Å, CRAF ras-binding domain chimera, apo form
9BP8 1.73 Å, Crystal structure of BRAF kinase domain with PF-07799933
5VYK 1.75 Å, Crystal structure of the BRS domain of BRAF in complex with the CC-SAM domain of KSR1
9BFB 1.92 Å, Crystal structure of BRAF kinase domain with PF-07284890
7K0V 1.93 Å, Crystal structure of bRaf in complex with inhibitor GNE-0749
5ITA 1.95 Å, Crystal Structure of BRAF Kinase Domain Bound to AZ-VEM
3NY5 1.99 Å, Crystal structure of the RBD domain of serine/threonine-protein kinase B-raf from Homo…
4XV9 2.0 Å, B-Raf Kinase domain in complex with PLX5568

Browse structure collections

About this viewer

MolViewer shows 8C7Y directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.