8D4V: Cathepsin G Inhibited by Eap2 from S. aureus

Crystal Structure of Cathepsin G Inhibited by Eap2 from S. aureus. Determined by X-ray diffraction at 1.85 Å resolution. Released 21 Dec 2022.

Method: X-ray diffraction
Resolution: 1.85 Å
Organisms: Homo sapiens, Staphylococcus aureus subsp. aureus Mu50
Chains: 4
Atoms: 5,312
Mol. weight: 73.03 kDa
Released: 21 Dec 2022

Explore 8D4V in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8D4V contains 26 α-helices and 64 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	1
β-strand	20-21	2	2
α-helix	22-23	2
β-strand	30-36	7	3
β-strand	41-50	10	3
β-strand	53-56	4	3
α-helix	58-60	3
β-strand	64-69	6	3
β-strand	73	1	4
β-strand	82-91	10	3
β-strand	96	1	5
β-strand	101	1	5
β-strand	105-109	5	3
α-helix	112-115	4
β-strand	116	1	6
β-strand	119	1	6
β-strand	123	1	2
α-helix	124-125	2
β-strand	136-141	6	2
β-strand	153	1	4
β-strand	155-161	7	2
α-helix	162-163	2
α-helix	165-168	4
β-strand	179-182	4	2
β-strand	190	1	1
β-strand	199-202	4	2
β-strand	205-213	9	2
α-helix	218	1
α-helix	220	1
β-strand	221-225	5	2
α-helix	226-229	4
α-helix	230-237	8

Chain B: 3 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	158-165	8	3
β-strand	166	1	13
β-strand	169	1	13
β-strand	170-171	2	2
β-strand	174-179	6	3
β-strand	184-186	3	14
α-helix	187-202	16
α-helix	206-211	6
β-strand	216-221	6	3
β-strand	226-230	5	3
β-strand	240-242	3	14
α-helix	243-245	3
β-strand	246-252	7	3

Chain C: 10 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	7
β-strand	20-21	2	8
α-helix	22-23	2
β-strand	30-36	7	9
β-strand	41-50	10	9
β-strand	53-56	4	9
α-helix	58-60	3
β-strand	64-69	6	9
β-strand	73	1	10
β-strand	82-91	10	9
β-strand	96	1	11
β-strand	101	1	11
β-strand	105-109	5	9
α-helix	112-115	4
β-strand	116	1	12
β-strand	119	1	12
β-strand	123	1	8
α-helix	124-125	2
β-strand	136-141	6	8
β-strand	153	1	10
β-strand	155-161	7	8
α-helix	162-163	2
α-helix	164-168	5
β-strand	179-182	4	8
β-strand	190	1	7
β-strand	199-202	4	8
β-strand	205-213	9	8
α-helix	218	1
α-helix	220	1
β-strand	221-225	5	8
α-helix	226-229	4
α-helix	230-237	8

Chain D: 3 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	157-165	9	9
β-strand	166	1	15
β-strand	169	1	15
β-strand	170-171	2	8
β-strand	174-180	7	9
β-strand	184-186	3	16
α-helix	187-202	16
α-helix	206-211	6
β-strand	216-221	6	9
β-strand	226-230	5	9
β-strand	240-242	3	16
α-helix	243-245	3
β-strand	246-252	7	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Cathepsin G, C-terminal truncated form	A, C	protein	223	Homo sapiens	P08311 (AlphaFold model)
Extracellular Adherence Protein	B, D	protein	100	Staphylococcus aureus subsp. aureus Mu50	Q99QS1 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>8D4V_1 Cathepsin G, C-terminal truncated form (chains A, C)
IIGGRESRPHSRPYMAYLQIQSPAGQSRCGGFLVREDFVLTAAHCWGSNINVTLGAHNIQ
RRENTQQHITARRAIRHPQYNQRTIQNDIMLLQLSRRVRRNRNVNPVALPRAQEGLRPGT
LCTVAGWGRVSMRRGTDTLREVQLRVQRDRQCLRIFGSYDPRRQICVGDRRERKAAFKGD
SGGPLLCNNVAHGIVSYGKSSGVPPEVFTRVSSFLPWIRTTMR

Sequence of entity 2 (B, D), FASTA

>8D4V_2 Extracellular Adherence Protein (chains B, D)
GSTVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQ
AKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVD

Primary citation

Characterization of two distinct neutrophil serine protease-binding modes within a Staphylococcus aureus innate immune evasion protein family. Gido, C.D., Herdendorf, T.J., Geisbrecht, B.V. J Biol Chem (2023) 299:102969-102969. DOI 10.1016/j.jbc.2023.102969 · PubMed

Other PDB entries of the same protein (UniProt P08311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7H6G 1.21 Å, THE 1.21 A CRYSTAL STRUCTURE OF HUMAN CATHEPSIN G IN COMPLEX WITH…
6VTM 1.6 Å, Human Cathepsin-G Inhibited by S. aureus EapH1
1CGH 1.8 Å, Human cathepsin G
8G25 1.8 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
8G24 1.82 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
1T32 1.85 Å, A Dual Inhibitor of the Leukocyte Proteases Cathepsin G and Chymase with Therapeutic…
8G26 1.85 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
1AU8 1.9 Å, Human cathepsin G
7H6H 1.94 Å, THE 1.94 A CRYSTAL STRUCTURE OF HUMAN CATHEPSIN G IN COMPLEX WITH…
8D4S 1.95 Å, Crystal Structure of Cathepsin G Inhibited by Eap1 from S. aureus
9ASX 1.96 Å, BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap3 of S. aureus
9ATK 2.11 Å, BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap4 of S. aureus

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