8EPL: PDB entry 8EPL

Human R-type voltage-gated calcium channel Cav2.3 at 3.1 Angstrom resolution. Determined by electron microscopy at 3.1 Å resolution. Released 14 Dec 2022.

Method
Electron microscopy
Resolution
3.1 Å
Organism
Homo sapiens
Chains
3
Atoms
20,953
Mol. weight
449.1 kDa
Ligands
CA, CLR, 3PE, PT5
Released
14 Dec 2022

Explore 8EPL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8EPL contains 118 α-helices and 84 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 66 helices, 13 β-strands

ElementResiduesLengthSheet
α-helix91-10717
β-strand11211
β-strand11611
α-helix119-1257
α-helix128-14316
α-helix159-17618
α-helix193-1986
α-helix199-2035
α-helix205-21814
α-helix221-24222
β-strand249-25132
β-strand26712
β-strand279-28132
α-helix295-30612
α-helix311-32212
α-helix328-3358
α-helix336-3416
α-helix342-35716
α-helix361-39737
α-helix472-4743
α-helix476-49318
α-helix502-52423
α-helix540-56021
α-helix569-58214
α-helix586-59813
α-helix601-62525
β-strand63113
β-strand63413
α-helix643-65513
α-helix659-66810
α-helix672-6754
α-helix677-6793
α-helix680-72748
α-helix777-78711
α-helix1145-11506
α-helix1156-117116
α-helix1181-120828
α-helix1224-124017
α-helix1252-12587
α-helix1259-12635
α-helix1265-12673
α-helix1268-12714
α-helix1273-131139
β-strand1316-131834
β-strand1332-133545
α-helix1337-13393
β-strand1342-134545
β-strand1348-135034
α-helix1358-137013
α-helix1374-13829
α-helix1397-13993
α-helix1400-140910
α-helix1410-14145
α-helix1415-142410
α-helix1443-145210
α-helix1468-14725
α-helix1478-149518
α-helix1504-153128
α-helix1534-15374
α-helix1541-155919
α-helix1573-15753
α-helix1576-15805
α-helix1583-15864
α-helix1591-160414
α-helix1610-162920
β-strand163216
α-helix1633-16342
α-helix1649-166012
α-helix1665-16717
β-strand167816
α-helix1699-172224
α-helix1723-17253
α-helix1726-17294
α-helix1741-175111
α-helix1767-17704
α-helix1783-17886
α-helix1796-17983
α-helix1806-181611
α-helix1829-183810
Chain B: 11 helices, 12 β-strands
ElementResiduesLengthSheet
α-helix39-5416
β-strand62-6657
β-strand91-9887
β-strand10318
β-strand104-10857
β-strand115-11627
β-strand11918
α-helix121-13313
α-helix156-1583
β-strand171-17447
β-strand180-18349
α-helix191-20717
β-strand212-21439
α-helix247-26216
β-strand266-26839
α-helix277-2804
β-strand288-29259
α-helix298-3058
α-helix309-3135
α-helix315-32612
α-helix330-3323
β-strand335-33739
α-helix342-35918
Chain C: 41 helices, 59 β-strands
ElementResiduesLengthSheet
α-helix30-5223
α-helix54-6411
β-strand68-72510
α-helix75-10935
α-helix1211
β-strand122-123211
β-strand125-126212
β-strand142-143211
β-strand150-151213
β-strand158-159213
β-strand165-167312
α-helix177-1859
α-helix186-1883
α-helix189-19911
β-strand206-210512
β-strand215-218412
α-helix238-2403
α-helix242-2487
β-strand252114
β-strand255-259515
α-helix262-2643
α-helix267-28014
β-strand289116
β-strand290-294515
β-strand298-300315
β-strand308116
α-helix312-32211
α-helix333-34311
β-strand355114
β-strand357-362615
α-helix370-3734
β-strand383-389715
α-helix397-4048
β-strand409-413515
α-helix417-4226
α-helix427-4304
α-helix432-4365
α-helix438-4414
β-strand445-446217
α-helix447-4493
β-strand450-451218
β-strand458-461418
β-strand462-463217
β-strand464-467412
β-strand484-490712
β-strand491-493318
α-helix494-4996
β-strand511-515519
β-strand520120
β-strand521-523319
α-helix531-5333
β-strand538-539221
β-strand560120
α-helix561-5644
α-helix569-58012
β-strand584-5931019
β-strand600-6111219
β-strand618-624719
β-strand629-633510
α-helix638-6458
α-helix650-6523
β-strand658-659222
α-helix679-68911
α-helix699-71517
α-helix716-7205
β-strand726122
β-strand729-736822
β-strand741-743322
α-helix746-7483
α-helix758-7603
α-helix762-7698
β-strand773123
β-strand774-776322
β-strand792-797622
β-strand800-801224
β-strand806-807224
β-strand810-816722
α-helix818-8269
β-strand848-853625
β-strand858126
β-strand859125
β-strand874126
α-helix875-8784
α-helix880-8889
β-strand892-899827
β-strand903-904228
β-strand907-908221
β-strand972-973228
β-strand976-983827
β-strand989-995725
β-strand1000-1007825
α-helix10081
β-strand1011123
β-strand1014-1019625
α-helix1028-10303
β-strand1036-1037227
α-helix10401
α-helix10421
α-helix1043-10464
α-helix1050-10523

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Voltage-dependent R-type calcium channel subunit alpha-1EAprotein2313Homo sapiensQ15878 (AlphaFold model)
Voltage-dependent L-type calcium channel subunit beta-3Bprotein484Homo sapiensP54284 (AlphaFold model)
Voltage-dependent calcium channel subunit alpha-2/delta-1Cprotein1103Homo sapiensP54289 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>8EPL_1 Voltage-dependent R-type calcium channel subunit alpha-1E (chains A)
MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQ
NCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPM
SRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHF
NTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGL
EFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVL
TVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERER
VENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRT
EAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYW
IVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSF
NCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSM
KSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWN
EVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEE
EAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQ
RTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGL
ALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAG
GGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDH
ELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVV
LTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPL
VDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTN
PIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFE
MVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVL
RVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTD
SSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQH
SVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLE
KNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYY
SAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILT
DSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFI
YAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEP
DTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHH
LDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAED
MTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKA
SDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPY
LQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSN
HGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSME
RSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRC
NSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLP
PVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQ
HASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPN
GHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC
Sequence of entity 2 (B), FASTA
>8EPL_2 Voltage-dependent L-type calcium channel subunit beta-3 (chains B)
MYDDSYVPGFEDSEAGSADSYTSRPSLDSDVSLEEDRESARREVESQAQQQLERAKHKPV
AFAVRTNVSYCGVLDEECPVQGSGVNFEAKDFLHIKEKYSNDWWIGRLVKEGGDIAFIPS
PQRLESIRLKQEQKARRSGNPSSLSDIGNRRSPPPSLAKQKQKQAEHVPPYDVVPSMRPV
VLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADLSLAKRSVLNNPGKRTIIER
SSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHPAQLAKTSLAPIIVFVKVSSPKVL
QRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVILDENQLEDACEHLAEYLEVYWRAT
HHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQR
SSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWP
KDSY
Sequence of entity 3 (C), FASTA
>8EPL_3 Voltage-dependent calcium channel subunit alpha-2/delta-1 (chains C)
MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDI
YEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASN
EVVYYNAKDDLDPEKNDSEPGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVL
NELNWTSALDEVFKKNREEDPSLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRR
RPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQD
VSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIML
FTDGGEERAQEIFNKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR
INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQFENKTNLK
NQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTIN
LRKRRPNIQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYI
DKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARYSETLKPDNFEESGYTF
IAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNADLINRVLLDAGFTNELVQNYW
SKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYF
NKSGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCD
CKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAFNKSYDY
QSVCEPGAAPKQGAGHRSAYVPSVADILQIGWWATAAAWSILQQFLLSLTFPRLLEAVEM
EDDDFTASLSKQSCITEQTQYFFDNDSKSFSGVLDCGNCSRIFHGEKLMNTNLIFIMVES
KGTCPCDTRLLIQAEQTSDGPNPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPS
LWYIIGIQFLLLWLVSGSTHRLL

Ligands and cofactors

IDNameFormulaCopies
CACalcium ionCa2
CLRCholesterolC27 H46 O5
3PE1,2-Distearoyl-sn-glycerophosphoethanolamineC41 H82 N O8 P2
PT5[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4…C47 H85 O19 P31
NAG2-acetamido-2-deoxy-beta-D-glucopyranoseC8 H15 N O63

Primary citation

Structures of the R-type human Ca v 2.3 channel reveal conformational crosstalk of the intracellular segments. Yao, X., Wang, Y., Wang, Z. et al. Nat Commun (2022) 13:7358-7358. DOI 10.1038/s41467-022-35026-6 · PubMed

Other PDB entries of the same protein (UniProt Q15878 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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