8F7A: Importin-9

Cryo-EM structure of Importin-9 bound to RanGTP. Determined by electron microscopy at 3.78 Å resolution. Released 26 Jul 2023.

Method
Electron microscopy
Resolution
3.78 Å
Organisms
Homo sapiens, Saccharomyces cerevisiae
Chains
2
Atoms
6,742
Mol. weight
141.44 kDa
Ligands
GTP, MG
Released
26 Jul 2023

Explore 8F7A in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8F7A contains 53 α-helices and 11 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 49 helices, 0 β-strands

ElementResiduesLengthSheet
α-helix27-337
α-helix37-5014
α-helix55-6511
α-helix71-8717
α-helix100-1023
α-helix103-11210
α-helix122-13817
α-helix148-1547
α-helix161-17616
α-helix182-19817
α-helix205-22824
α-helix238-24912
α-helix262-27817
α-helix280-2834
α-helix287-30519
α-helix306-3105
α-helix329-34315
α-helix347-3559
α-helix357-36711
α-helix370-3712
α-helix372-3809
α-helix382-3887
α-helix398-41215
α-helix414-43724
α-helix444-45512
α-helix458-4669
α-helix474-4763
α-helix477-4837
α-helix484-4863
α-helix492-50413
α-helix505-5073
α-helix511-52212
α-helix523-5253
α-helix531-55121
α-helix554-5563
α-helix558-5603
α-helix561-57212
α-helix578-59316
α-helix595-61521
α-helix620-63415
α-helix637-6393
α-helix640-65617
α-helix6631
α-helix666-67914
α-helix685-6862
α-helix687-6882
α-helix689-6935
α-helix694-70310
α-helix707-72014
Chain B: 4 helices, 11 β-strands
ElementResiduesLengthSheet
β-strand12-1651
β-strand1712
α-helix26-338
β-strand47-5041
β-strand53-5641
β-strand59-68101
β-strand8813
β-strand8912
β-strand9314
α-helix98-1014
α-helix103-11311
β-strand11913
β-strand12414
β-strand14613
α-helix161-17111

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Importin-9Aprotein1041Homo sapiensQ96P70 (AlphaFold model)
GTP-binding nuclear protein GSP1/CNR1Bprotein219Saccharomyces cerevisiaeP32835 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>8F7A_1 Importin-9 (chains A)
MAAAAAAGAASGLPGPVAQGLKEALVDTLTGILSPVQEVRAAAEEQIKVLEVTEEFGVHL
AELTVDPQGALAIRQLASVILKQYVETHWCAQSEKFRPPETTERAKIVIRELLPNGLRES
ISKVRSSVAYAVSAIAHWDWPEAWPQLFNLLMEMLVSGDLNAVHGAMRVLTEFTREVTDT
QMPLVAPVILPEMYKIFTMAEVYGIRTRSRAVEIFTTCAHMICNMEELEKGAAKVLIFPV
VQQFTEAFVQALQIPDGPTSDSGFKMEVLKAVTALVKNFPKHMVSSMQQILPIVWNTLTE
SAAFYVRTEVNYTEEVEDPVDSDGEVLGFENLVFSIFEFVHALLENSKFKSTVKKALPEL
IYYIILYMQITEEQIKVWTANPQQFVEDEDDDTFSYTVRIAAQDLLLAVATDFQNESAAA
LAAAATRHLQEAEQTKNSGTEHWWKIHEACMLALGSVKAIITDSVKNGRIHFDMHGFLTN
VILADLNLSVSPFLLGRALWAASRFTVAMSPELIQQFLQATVSGLHETQPPSVRISAVRA
IWGYCDQLKVSESTHVLQPFLPSILDGLIHLAAQFSSEVLNLVMETLCIVCTVDPEFTAS
MESKICPFTIAIFLKYSNDPVVASLAQDIFKELSQIEACQGPMQMRLIPTLVSIMQAPAD
KIPAGLCATAIDILTTVVRNTKPPLSQLLICQAFPAVAQCTLHTDDNATMQNGGECLRAY
VSVTLEQVAQWHDEQGHNGLWYVMQVVSQLLDPRTSEFTAAFVGRLVSTLISKAGRELGE
NLDQILRAILSKMQQAETLSVMQSLIMVFAHLVHTQLEPLLEFLCSLPGPTGKPALEFVM
AEWTSRQHLFYGQYEGKVSSVALCKLLQHGINADDKRLQDIRVKGEEIYSMDEGIRTRSK
SAKNPERWTNIPLLVKILKLIINELSNVMEANAARQATPAEWSQDDSNDMWEDQEEEEEE
EEDGLAGQLLSDILATSKYEEDYYEDDEEDDPDALKDPLYQIDLQAYLTDFLCQFAQQPC
YIMFSGHLNDNERRVLQTIGI
Sequence of entity 2 (B), FASTA
>8F7A_2 GTP-binding nuclear protein GSP1/CNR1 (chains B)
MSAPAANGEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGE
IKFDVWDTAGLEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIV
LCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLEFVA
SPALAPPEVQVDEQLMQQYQQEMEQATALPLPDEDDADL

Ligands and cofactors

IDNameFormulaCopies
GTPGuanosine-5'-triphosphateC10 H16 N5 O14 P31
MGMagnesium ionMg1

Primary citation

Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex. Jiou, J., Shaffer, J.M., Bernades, N.E. et al. Proc Natl Acad Sci U S A (2023) 120:e2301199120-e2301199120. DOI 10.1073/pnas.2301199120 · PubMed

Other PDB entries of the same protein (UniProt Q96P70 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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