Cryo-EM structure of Importin-9 bound to RanGTP. Determined by electron microscopy at 3.78 Å resolution. Released 26 Jul 2023.
Explore 8F7A in 3D Show helices and sheets RCSB PDB PDBe
8F7A contains 53 α-helices and 11 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 27-33 | 7 | |
| α-helix | 37-50 | 14 | |
| α-helix | 55-65 | 11 | |
| α-helix | 71-87 | 17 | |
| α-helix | 100-102 | 3 | |
| α-helix | 103-112 | 10 | |
| α-helix | 122-138 | 17 | |
| α-helix | 148-154 | 7 | |
| α-helix | 161-176 | 16 | |
| α-helix | 182-198 | 17 | |
| α-helix | 205-228 | 24 | |
| α-helix | 238-249 | 12 | |
| α-helix | 262-278 | 17 | |
| α-helix | 280-283 | 4 | |
| α-helix | 287-305 | 19 | |
| α-helix | 306-310 | 5 | |
| α-helix | 329-343 | 15 | |
| α-helix | 347-355 | 9 | |
| α-helix | 357-367 | 11 | |
| α-helix | 370-371 | 2 | |
| α-helix | 372-380 | 9 | |
| α-helix | 382-388 | 7 | |
| α-helix | 398-412 | 15 | |
| α-helix | 414-437 | 24 | |
| α-helix | 444-455 | 12 | |
| α-helix | 458-466 | 9 | |
| α-helix | 474-476 | 3 | |
| α-helix | 477-483 | 7 | |
| α-helix | 484-486 | 3 | |
| α-helix | 492-504 | 13 | |
| α-helix | 505-507 | 3 | |
| α-helix | 511-522 | 12 | |
| α-helix | 523-525 | 3 | |
| α-helix | 531-551 | 21 | |
| α-helix | 554-556 | 3 | |
| α-helix | 558-560 | 3 | |
| α-helix | 561-572 | 12 | |
| α-helix | 578-593 | 16 | |
| α-helix | 595-615 | 21 | |
| α-helix | 620-634 | 15 | |
| α-helix | 637-639 | 3 | |
| α-helix | 640-656 | 17 | |
| α-helix | 663 | 1 | |
| α-helix | 666-679 | 14 | |
| α-helix | 685-686 | 2 | |
| α-helix | 687-688 | 2 | |
| α-helix | 689-693 | 5 | |
| α-helix | 694-703 | 10 | |
| α-helix | 707-720 | 14 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 12-16 | 5 | 1 |
| β-strand | 17 | 1 | 2 |
| α-helix | 26-33 | 8 | |
| β-strand | 47-50 | 4 | 1 |
| β-strand | 53-56 | 4 | 1 |
| β-strand | 59-68 | 10 | 1 |
| β-strand | 88 | 1 | 3 |
| β-strand | 89 | 1 | 2 |
| β-strand | 93 | 1 | 4 |
| α-helix | 98-101 | 4 | |
| α-helix | 103-113 | 11 | |
| β-strand | 119 | 1 | 3 |
| β-strand | 124 | 1 | 4 |
| β-strand | 146 | 1 | 3 |
| α-helix | 161-171 | 11 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Importin-9 | A | protein | 1041 | Homo sapiens | Q96P70 (AlphaFold model) |
| GTP-binding nuclear protein GSP1/CNR1 | B | protein | 219 | Saccharomyces cerevisiae | P32835 (AlphaFold model) |
>8F7A_1 Importin-9 (chains A) MAAAAAAGAASGLPGPVAQGLKEALVDTLTGILSPVQEVRAAAEEQIKVLEVTEEFGVHL AELTVDPQGALAIRQLASVILKQYVETHWCAQSEKFRPPETTERAKIVIRELLPNGLRES ISKVRSSVAYAVSAIAHWDWPEAWPQLFNLLMEMLVSGDLNAVHGAMRVLTEFTREVTDT QMPLVAPVILPEMYKIFTMAEVYGIRTRSRAVEIFTTCAHMICNMEELEKGAAKVLIFPV VQQFTEAFVQALQIPDGPTSDSGFKMEVLKAVTALVKNFPKHMVSSMQQILPIVWNTLTE SAAFYVRTEVNYTEEVEDPVDSDGEVLGFENLVFSIFEFVHALLENSKFKSTVKKALPEL IYYIILYMQITEEQIKVWTANPQQFVEDEDDDTFSYTVRIAAQDLLLAVATDFQNESAAA LAAAATRHLQEAEQTKNSGTEHWWKIHEACMLALGSVKAIITDSVKNGRIHFDMHGFLTN VILADLNLSVSPFLLGRALWAASRFTVAMSPELIQQFLQATVSGLHETQPPSVRISAVRA IWGYCDQLKVSESTHVLQPFLPSILDGLIHLAAQFSSEVLNLVMETLCIVCTVDPEFTAS MESKICPFTIAIFLKYSNDPVVASLAQDIFKELSQIEACQGPMQMRLIPTLVSIMQAPAD KIPAGLCATAIDILTTVVRNTKPPLSQLLICQAFPAVAQCTLHTDDNATMQNGGECLRAY VSVTLEQVAQWHDEQGHNGLWYVMQVVSQLLDPRTSEFTAAFVGRLVSTLISKAGRELGE NLDQILRAILSKMQQAETLSVMQSLIMVFAHLVHTQLEPLLEFLCSLPGPTGKPALEFVM AEWTSRQHLFYGQYEGKVSSVALCKLLQHGINADDKRLQDIRVKGEEIYSMDEGIRTRSK SAKNPERWTNIPLLVKILKLIINELSNVMEANAARQATPAEWSQDDSNDMWEDQEEEEEE EEDGLAGQLLSDILATSKYEEDYYEDDEEDDPDALKDPLYQIDLQAYLTDFLCQFAQQPC YIMFSGHLNDNERRVLQTIGI
>8F7A_2 GTP-binding nuclear protein GSP1/CNR1 (chains B) MSAPAANGEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGE IKFDVWDTAGLEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIV LCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLEFVA SPALAPPEVQVDEQLMQQYQQEMEQATALPLPDEDDADL
Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex. Jiou, J., Shaffer, J.M., Bernades, N.E. et al. Proc Natl Acad Sci U S A (2023) 120:e2301199120-e2301199120. DOI 10.1073/pnas.2301199120 · PubMed
Other PDB entries of the same protein (UniProt Q96P70 (AlphaFold model), which also has an AlphaFold model), best resolution first:
MolViewer shows 8F7A directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.