8FHS: PDB entry 8FHS

Human L-type voltage-gated calcium channel Cav1.2 in the presence of amiodarone and sofosbuvir at 3.3 Angstrom resolution. Determined by electron microscopy at 3.3 Å resolution. Released 13 Dec 2023.

Method
Electron microscopy
Resolution
3.3 Å
Organism
Homo sapiens
Chains
3
Atoms
20,975
Mol. weight
436.01 kDa
Ligands
CA, BBI, WG6, 3PE
Released
13 Dec 2023

Explore 8FHS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8FHS contains 116 α-helices and 75 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 63 helices, 10 β-strands

ElementResiduesLengthSheet
α-helix113-1164
α-helix129-14315
α-helix146-1472
α-helix154-18027
α-helix194-21623
α-helix234-2429
α-helix243-2508
α-helix252-26312
α-helix269-28921
β-strand296-29941
β-strand331-33441
α-helix349-36012
α-helix365-37511
α-helix382-3898
α-helix390-3956
α-helix396-41823
α-helix420-44526
α-helix509-52113
α-helix526-54116
α-helix550-57829
α-helix580-5834
α-helix589-60719
α-helix618-62912
α-helix634-64613
α-helix648-67225
α-helix692-70312
α-helix708-71811
α-helix724-7274
α-helix730-78152
α-helix900-91819
α-helix928-97245
α-helix990-100011
α-helix1001-10033
α-helix1013-10175
α-helix1019-10235
α-helix1025-10284
α-helix1035-107036
β-strand1077-107822
β-strand1092-109653
α-helix1097-10993
β-strand1104-110853
β-strand1111-111222
α-helix1121-113212
α-helix1137-11459
β-strand114714
β-strand115414
α-helix1160-11623
α-helix1163-11697
α-helix1170-11778
α-helix1178-119417
α-helix1206-12149
α-helix1218-12203
α-helix1227-123711
α-helix1239-125618
α-helix1265-129329
α-helix1295-12984
α-helix1302-132120
α-helix1377-13826
α-helix1383-13886
α-helix1392-140716
α-helix1409-143022
β-strand143315
α-helix1434-14352
α-helix1450-146213
α-helix1466-14727
β-strand147915
α-helix1500-152425
α-helix1527-15304
α-helix1539-154911
α-helix1565-15717
α-helix1586-15938
α-helix1606-161712
α-helix1626-163510
Chain C: 11 helices, 10 β-strands
ElementResiduesLengthSheet
α-helix40-5415
β-strand63-66423
β-strand91-95523
β-strand103-108623
β-strand116-119423
α-helix121-13313
α-helix156-1583
β-strand172-174323
β-strand180-183424
α-helix191-20717
β-strand212-215424
α-helix247-26216
β-strand266-269424
α-helix279-2813
β-strand288-291424
α-helix297-3059
α-helix309-3124
α-helix317-32610
α-helix330-3323
β-strand335-336224
α-helix342-35918
Chain D: 42 helices, 55 β-strands
ElementResiduesLengthSheet
α-helix30-5223
α-helix55-639
β-strand68-7256
α-helix75-10935
β-strand12217
β-strand125-12628
β-strand14217
β-strand150-15129
β-strand158-15929
β-strand160110
β-strand165-16738
α-helix177-1859
α-helix186-1883
α-helix189-19911
β-strand206-21058
β-strand215-21848
β-strand220110
α-helix238-2403
α-helix242-2487
β-strand252111
β-strand255-259512
α-helix262-2643
α-helix267-28014
β-strand289113
β-strand291-294412
β-strand298-300312
β-strand308113
α-helix312-32211
α-helix333-34311
β-strand355111
β-strand357-362612
α-helix370-3756
β-strand383-389712
α-helix397-4048
β-strand409-413512
α-helix416-4183
α-helix419-4235
α-helix426-4305
α-helix432-4365
α-helix438-4414
β-strand450-451214
β-strand458-461414
β-strand464-46748
β-strand484-49078
β-strand491-493314
α-helix494-4996
β-strand511-515515
β-strand520116
β-strand521-523315
α-helix531-5333
β-strand560116
α-helix561-5644
α-helix569-58012
β-strand584-5931015
β-strand599117
β-strand600-6111215
β-strand618-622515
β-strand629-63356
α-helix638-6458
α-helix646-6483
α-helix650-6523
α-helix653-6564
β-strand658-659218
α-helix678-68912
α-helix699-71517
α-helix716-7205
β-strand731-736618
β-strand741-743318
α-helix746-7494
α-helix758-7603
α-helix762-7698
β-strand773119
β-strand774-776318
β-strand792-797618
β-strand800-801220
β-strand806-807220
β-strand810-816718
α-helix818-8269
β-strand848-854721
β-strand858122
β-strand859-861321
β-strand874122
α-helix875-8773
α-helix880-8889
β-strand892-9041317
β-strand972-9831217
β-strand989-994621
β-strand1001-1007721
α-helix10081
β-strand1011119
β-strand1013-1019721
α-helix1021-10233
α-helix1029-10313
β-strand1036-1038317
α-helix1043-10464
α-helix1050-10523
α-helix1072-10754

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Voltage-dependent L-type calcium channel subunit alpha-1CAprotein2221Homo sapiensQ13936 (AlphaFold model)
Voltage-dependent calcium channel subunit alpha-2/delta-1Dprotein1103Homo sapiensP54289 (AlphaFold model)
Voltage-dependent L-type calcium channel subunit beta-3Cprotein484Homo sapiensP54284 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>8FHS_1 Voltage-dependent L-type calcium channel subunit alpha-1C (chains A)
MVNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAAR
QAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISI
VEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIA
YGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFR
VLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYN
QEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCI
TMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGD
FQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVA
GGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLT
IASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGI
LETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFL
FIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYG
GPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLAR
TASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNP
ETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDT
IFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKILGNADYVF
TSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVL
RVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSD
SSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPEL
LYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNC
ELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQH
YGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVIL
SETNHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFR
VMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTE
INRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFA
VFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRI
KHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRI
KTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFK
KRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASE
DDIFRRAGGLFGNHVSYYQSDGRSAFPQTFTTQRPLHINKAGSSQGDTESPSHEKLVDST
FTPSSYSSTGSNANINNANNTALGRLPRPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLS
SNRERHVPMCEDLELRRDSGSAGTQAHCLLLRKANPSRCHSRESQAAMAGQEETSQDETY
EVKMNHDTEACSEPSLLSTEMLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRA
SFHLECLKRQKDRGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPPA
TPGSRGWPPQPVPTLRLEGVESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVSLM
VPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMESA
ADNILSGGAPQSPNGALLPFVNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRVYVSS
L
Sequence of entity 2 (D), FASTA
>8FHS_2 Voltage-dependent calcium channel subunit alpha-2/delta-1 (chains D)
MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDI
YEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASN
EVVYYNAKDDLDPEKNDSEPGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVL
NELNWTSALDEVFKKNREEDPSLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRR
RPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQD
VSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIML
FTDGGEERAQEIFNKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR
INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQFENKTNLK
NQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTIN
LRKRRPNIQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYI
DKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARYSETLKPDNFEESGYTF
IAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNADLINRVLLDAGFTNELVQNYW
SKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYF
NKSGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCD
CKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAFNKSYDY
QSVCEPGAAPKQGAGHRSAYVPSVADILQIGWWATAAAWSILQQFLLSLTFPRLLEAVEM
EDDDFTASLSKQSCITEQTQYFFDNDSKSFSGVLDCGNCSRIFHGEKLMNTNLIFIMVES
KGTCPCDTRLLIQAEQTSDGPNPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPS
LWYIIGIQFLLLWLVSGSTHRLL
Sequence of entity 3 (C), FASTA
>8FHS_3 Voltage-dependent L-type calcium channel subunit beta-3 (chains C)
MYDDSYVPGFEDSEAGSADSYTSRPSLDSDVSLEEDRESARREVESQAQQQLERAKHKPV
AFAVRTNVSYCGVLDEECPVQGSGVNFEAKDFLHIKEKYSNDWWIGRLVKEGGDIAFIPS
PQRLESIRLKQEQKARRSGNPSSLSDIGNRRSPPPSLAKQKQKQAEHVPPYDVVPSMRPV
VLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADLSLAKRSVLNNPGKRTIIER
SSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHPAQLAKTSLAPIIVFVKVSSPKVL
QRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVILDENQLEDACEHLAEYLEVYWRAT
HHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQR
SSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWP
KDSY

Ligands and cofactors

IDNameFormulaCopies
CACalcium ionCa2
BBI(2-butyl-1-benzofuran-3-yl){4-[2-(diethylamino)ethoxy]-3,5-diiodophenyl}methano…C25 H29 I2 N O31
WG6SofosbuvirC22 H29 F N3 O9 P1
3PE1,2-Distearoyl-sn-glycerophosphoethanolamineC41 H82 N O8 P2
CLRCholesterolC27 H46 O4
NAG2-acetamido-2-deoxy-beta-D-glucopyranoseC8 H15 N O62

Primary citation

Structural basis for human Ca v 1.2 inhibition by multiple drugs and the neurotoxin calciseptine. Gao, S., Yao, X., Chen, J. et al. Cell (2023) 186:5363-5374.e16. DOI 10.1016/j.cell.2023.10.007 · PubMed

Other PDB entries of the same protein (UniProt Q13936 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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