8FPG: GluA2 flip Q isoform of AMPA receptor

GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100uM CNQX (Closed-CaNaMg). Determined by electron microscopy at 2.32 Å resolution. Released 28 Feb 2024.

Method: Electron microscopy
Resolution: 2.32 Å
Organisms: Mus musculus, Rattus norvegicus
Chains: 8
Atoms: 10,674
Mol. weight: 546.82 kDa
Released: 28 Feb 2024

Explore 8FPG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8FPG contains 56 α-helices and 28 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	516-518	3
β-strand	521	1	5
α-helix	523-545	23
α-helix	548-550	3
α-helix	573-584	12
α-helix	596-628	33
β-strand	787	1	6
α-helix	788	1
α-helix	789-791	3
α-helix	793-824	32

Chain B: 10 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	509-513	5
α-helix	516-518	3
β-strand	521	1	4
α-helix	523-545	23
α-helix	548-550	3
α-helix	573-584	12
α-helix	596-625	30
α-helix	786	1
β-strand	787	1	5
α-helix	788	1
α-helix	789-791	3
α-helix	793-824	32

Chain C: 8 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	516-518	3
β-strand	521	1	3
α-helix	523-545	23
α-helix	548-550	3
α-helix	573-585	13
α-helix	596-628	33
β-strand	787	1	4
α-helix	788	1
α-helix	789-792	4
α-helix	793-824	32

Chain D: 10 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	509-513	5
α-helix	516-518	3
β-strand	521	1	6
α-helix	523-545	23
α-helix	548-550	3
α-helix	573-584	12
α-helix	596-625	30
α-helix	786	1
β-strand	787	1	3
α-helix	788	1
α-helix	789-792	4
α-helix	793-824	32

Chains E and G: 5 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	7-29	23
β-strand	34-38	5	7
β-strand	57-61	5	7
β-strand	65-68	4	7
β-strand	77-79	3	7
α-helix	93-104	12
α-helix	106-124	19
α-helix	133-160	28
β-strand	175	1	7
α-helix	178-213	36

Chains F and H: 5 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	6-29	24
β-strand	34-38	5	2
β-strand	57-61	5	2
β-strand	65-68	4	2
β-strand	77-79	3	2
α-helix	93-104	12
α-helix	106-127	22
α-helix	133-161	29
β-strand	175-176	2	2
α-helix	178-213	36

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Voltage-dependent calcium channel gamma-2 subunit	E, F, G, H	protein	336	Mus musculus	O88602 (AlphaFold model)
Glutamate receptor 2	A, B, C, D	protein	889	Rattus norvegicus	P19491 (AlphaFold model)

Sequence of entity 1 (E, F, G, H), FASTA

>8FPG_1 Voltage-dependent calcium channel gamma-2 subunit (chains E, F, G, H)
MGLFDRGVQMLLTTVGAFAAFSLMTIAVGTDYWLYSRGVCKTKSVSENETSEENEEVMTH
SGLWRTCCLEGNFKGLCKQIDHFPEDADYEADTAEYFLRAVRASSIFPILSVILLFMGGL
CIAASEFYKTRHNIILSAGIFFVSAGLSNIIGIIVYISANAGDPSKSDSKKNSYSYGWSF
YFGALSFIIAEMVGVLAVHMFIDRHKQLRATARATDYLQASAITRIPSYRYRYQRRSRSS
SRSTEPSHSRDASPVGVKGFNTLPSTEISMYTLSRDPLKAATTPTATYNSDRDNSFLQVH
NCIQKDSKDSLHANTANRRTTPVGGRGGTETSQAPA

Sequence of entity 2 (A, B, C, D), FASTA

>8FPG_2 Glutamate receptor 2 (chains A, B, C, D)
MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTP
HIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDG
THPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINV
GNINNDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANL
GFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALT
YDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNI
KFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTT
ILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKI
WNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLD
PLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWF
SLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDL
SKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGK
YAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGTPVNLAVLKLSEQGVL
DKLKNKWWYDKGECGAKDSGSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSR
AEAKRMKVAKNPQNINPSSSQNSQNFATDYKDDDDKEGYNVYGIESVKI

Primary citation

The open gate of the AMPA receptor forms a Ca 2+ binding site critical in regulating ion transport. Nakagawa, T., Wang, X.T., Miguez-Cabello, F.J. et al. Nat Struct Mol Biol (2024) 31:688-700. DOI 10.1038/s41594-024-01228-3 · PubMed

Other PDB entries of the same protein (UniProt O88602 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3JXT 1.5 Å, Crystal structure of the third PDZ domain of SAP-102 in complex with a fluorogenic…
8FQF 2.29 Å, GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2,…
8VHV 2.3 Å, Transmembrane AMPA Receptor Regulatory Protein Subunit Gamma 2
8FQ5 2.34 Å, GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with…
8FQB 2.36 Å, GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with…
8FPS 2.38 Å, GluA2 flip Q isoform N619K mutant of AMPA receptor in complex with gain-of-function TARP…
8FP4 2.4 Å, GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2,…
4X3H 2.4 Å, Crystal structure of arc N-lobe complexed with stargazin peptide
8FP9 2.44 Å, GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2,…
9B60 2.57 Å, GluA2 flip Q in complex with TARPgamma2 at pH8, consensus structure of TMD-TARPgamma2
9B5Z 2.71 Å, GluA2 flip Q in complex with TARPgamma2 at pH8, consensus structure of LBD-TMD-TARPgamma2
9B63 2.76 Å, GluA2 flip Q in complex with TARPgamma2 at pH5, consensus structure of TMD-TARPgamma2

Browse structure collections

About this viewer

MolViewer shows 8FPG directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.