8H0Q: GRP14-27-GRPR-Gq complex
Structure of the GRP14-27-GRPR-Gq complex. Determined by electron microscopy at 3.3 Å resolution. Released 9 Aug 2023.
- Method
- Electron microscopy
- Resolution
- 3.3 Å
- Organisms
- Homo sapiens, Mus musculus
- Chains
- 6
- Atoms
- 8,949
- Mol. weight
- 159.12 kDa
- Ligands
- CLR
- Released
- 9 Aug 2023
Explore 8H0Q in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8H0Q contains 29 α-helices and 67 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 9 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 13-38 | 26 | |
| β-strand | 39-46 | 8 | 3 |
| α-helix | 52-58 | 7 | |
| β-strand | 189-196 | 8 | 3 |
| β-strand | 199-206 | 8 | 3 |
| α-helix | 216-220 | 5 | |
| β-strand | 225-231 | 7 | 3 |
| α-helix | 248-259 | 12 | |
| α-helix | 262-264 | 3 | |
| β-strand | 268-274 | 7 | 3 |
| α-helix | 276-283 | 8 | |
| α-helix | 293-296 | 4 | |
| α-helix | 308-317 | 10 | |
| β-strand | 325-328 | 4 | 3 |
| α-helix | 336-355 | 20 | |
Chain B: 3 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-29 | 21 | |
| α-helix | 35-38 | 4 | |
| β-strand | 52-56 | 5 | 4 |
| β-strand | 63-67 | 5 | 5 |
| β-strand | 74-79 | 6 | 5 |
| β-strand | 83-88 | 6 | 5 |
| β-strand | 94-99 | 6 | 5 |
| β-strand | 105-110 | 6 | 6 |
| β-strand | 116-121 | 6 | 6 |
| β-strand | 125-130 | 6 | 6 |
| β-strand | 140-145 | 6 | 6 |
| β-strand | 154 | 1 | 7 |
| β-strand | 161-165 | 5 | 7 |
| β-strand | 171-175 | 5 | 7 |
| β-strand | 181-184 | 4 | 7 |
| α-helix | 185-187 | 3 | |
| β-strand | 194-197 | 4 | 8 |
| β-strand | 203-207 | 5 | 8 |
| β-strand | 213-217 | 5 | 8 |
| β-strand | 225-227 | 3 | 8 |
| β-strand | 234-239 | 6 | 9 |
| β-strand | 245-250 | 6 | 9 |
| β-strand | 255-259 | 5 | 9 |
| β-strand | 265-269 | 5 | 9 |
| β-strand | 278-283 | 6 | 10 |
| β-strand | 289-294 | 6 | 10 |
| β-strand | 299-303 | 5 | 10 |
| β-strand | 309-313 | 5 | 10 |
| β-strand | 320-325 | 6 | 4 |
| β-strand | 332-336 | 5 | 4 |
| β-strand | 341-344 | 4 | 4 |
Chain E: 1 helix, 29 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-6 | 5 | 11 |
| β-strand | 9-11 | 3 | 12 |
| β-strand | 16-24 | 9 | 11 |
| α-helix | 32-33 | 2 | |
| β-strand | 34-38 | 5 | 13 |
| β-strand | 44-50 | 7 | 13 |
| β-strand | 57-59 | 3 | 13 |
| β-strand | 67-72 | 6 | 11 |
| β-strand | 77-83 | 7 | 11 |
| β-strand | 92-96 | 5 | 13 |
| β-strand | 110 | 1 | 13 |
| β-strand | 114 | 1 | 13 |
| β-strand | 116-118 | 3 | 12 |
| β-strand | 142 | 1 | 14 |
| β-strand | 155-160 | 6 | 14 |
| β-strand | 166 | 1 | 15 |
| β-strand | 172 | 1 | 15 |
| β-strand | 174 | 1 | 16 |
| β-strand | 176-178 | 3 | 17 |
| β-strand | 179 | 1 | 18 |
| β-strand | 186-189 | 4 | 17 |
| β-strand | 195 | 1 | 17 |
| β-strand | 203-207 | 5 | 14 |
| β-strand | 211-216 | 6 | 14 |
| β-strand | 226 | 1 | 18 |
| β-strand | 227 | 1 | 19 |
| β-strand | 230 | 1 | 20 |
| β-strand | 231 | 1 | 16 |
| β-strand | 239 | 1 | 20 |
| β-strand | 243 | 1 | 19 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-22 | 17 | |
| α-helix | 29-42 | 14 | |
| α-helix | 52-54 | 3 | |
| α-helix | 55-57 | 3 | |
Chain R: 12 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 43-67 | 25 | |
| α-helix | 74-101 | 28 | |
| α-helix | 112-138 | 27 | |
| α-helix | 139-143 | 5 | |
| α-helix | 154-177 | 24 | |
| β-strand | 180-181 | 2 | 1 |
| β-strand | 184-185 | 2 | 2 |
| β-strand | 192-193 | 2 | 2 |
| β-strand | 196-197 | 2 | 1 |
| α-helix | 205-215 | 11 | |
| α-helix | 216-220 | 5 | |
| α-helix | 221-240 | 20 | |
| α-helix | 250-289 | 40 | |
| α-helix | 298-317 | 20 | |
| α-helix | 321-325 | 5 | |
| α-helix | 330-338 | 9 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Gastrin-releasing peptide receptor | R | protein | 384 | Homo sapiens | P30550 (AlphaFold model) |
| GRP | L | protein | 9 | Homo sapiens | P07492 (AlphaFold model) |
| G-alpha q | A | protein | 361 | Homo sapiens | P63092 (AlphaFold model), P63096 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 343 | Homo sapiens | P62873 |
| scFv16 | E | protein | 247 | Mus musculus | |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 |
Sequence of entity 1 (R), FASTA
>8H0Q_1 Gastrin-releasing peptide receptor (chains R)
MALNDCFLLNLEVDHFMHCNISSHSADLPVNDDWSHPGILYVIPAVYGVIILIGLIGNIT
LIKIFCTVKSMRNVPNLFISSLALGDLLLLITCAPVDASRYLADRWLFGRIGCKLIPFIQ
LTSVGVSVFTQTALSADRYKAIVRPMDIQASHALMKICLKAAFIWIISMLLAIPEAVFSD
LHPFHEESTNQTFISCAPYPHSNELHPKIHSMASFLVFYVIPLSIISVYYYFIAKNLIQS
AYNLPVEGNIHVKKQIESRKRLAKTVLVFVGLFAFCWLPNHVIYLYRSYHYSEVDTSMLH
FVTSICARLLAFTNSCVNPFALYLLSKSFRKQFNTQLLCCQPGLIIRSHSTGRSTTCMTS
LKSTNPSVATFSLINGNICHERYV
Sequence of entity 2 (L), FASTA
>8H0Q_2 GRP (chains L)
NHWAVGHLM
Sequence of entity 3 (A), FASTA
>8H0Q_3 G-alpha q (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRKEFVDISTASGDGRHICYPHFTCSVDTENARRIFNDCKDIILQMNLREYNL
V
Sequence of entity 4 (B), FASTA
>8H0Q_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAK
IYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGL
DNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQT
TTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPN
GNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVW
DALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 5 (E), FASTA
>8H0Q_5 scFv16 (chains E)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL
Sequence of entity 6 (G), FASTA
>8H0Q_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CLR | Cholesterol | C27 H46 O | 3 |
Primary citation
Molecular recognition of itch-associated neuropeptides by bombesin receptors. Li, C., Xu, Y., Liu, H. et al. Cell Res (2023) 33:184-187. DOI 10.1038/s41422-022-00743-6
Other PDB entries of the same protein (UniProt P30550 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7W41 2.95 Å, Crystal Structure of Human Gastrin Releasing Peptide Receptor in complex with the…
- 7W3Z 3.0 Å, Cryo-EM Structure of Human Gastrin Releasing Peptide Receptor in complex with the…
- 7W40 3.0 Å, Cryo-EM Structure of Human Gastrin Releasing Peptide Receptor in complex with the…
Browse structure collections
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