8I0P: Human pre-Bact complex

The cryo-EM structure of human pre-Bact complex. Determined by electron microscopy at 3.4 Å resolution. Released 31 Jul 2024.

Method: Electron microscopy
Resolution: 3.4 Å
Organisms: Homo sapiens, unidentified adenovirus
Chains: 51
Atoms: 103,887
Mol. weight: 2845.66 kDa
Ligands: ZN, MG, GTP
Released: 31 Jul 2024

Explore 8I0P in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8I0P contains 634 α-helices and 611 β-strands across 47 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 1: 72 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	89-92	4
α-helix	103-106	4
α-helix	109-110	2
β-strand	111	1	125
α-helix	120-123	4
α-helix	131	1
β-strand	132	1	126
α-helix	133-134	2
α-helix	141-144	4
β-strand	150	1	126
α-helix	152-175	24
α-helix	401-407	7
β-strand	412-414	3	73
α-helix	421-430	10
α-helix	452-455	4
α-helix	472-475	4
α-helix	476-479	4
α-helix	490-498	9
α-helix	501-505	5
α-helix	509-521	13
α-helix	523-526	4
α-helix	528-539	12
β-strand	545	1	125
α-helix	546-562	17
α-helix	568-570	3
α-helix	571-578	8
α-helix	579-582	4
α-helix	587-603	17
α-helix	606-613	8
α-helix	622-639	18
α-helix	641-652	12
α-helix	658-675	18
α-helix	676-682	7
α-helix	683-691	9
α-helix	699-715	17
α-helix	721-724	4
α-helix	725-727	3
α-helix	728-737	10
α-helix	741-753	13
α-helix	759-776	18
α-helix	782-795	14
α-helix	803-809	7
α-helix	811-818	8
α-helix	821-825	5
α-helix	828-844	17
α-helix	846-853	8
α-helix	855-858	4
α-helix	862-879	18
α-helix	886-900	15
α-helix	908-921	14
α-helix	922-928	7
α-helix	929-941	13
α-helix	945-964	20
α-helix	968-981	14
α-helix	987-1001	15
α-helix	1006-1008	3
α-helix	1013-1020	8
α-helix	1021-1026	6
α-helix	1029-1045	17
α-helix	1052-1062	11
α-helix	1065-1067	3
α-helix	1071-1088	18
α-helix	1090-1098	9
α-helix	1110-1122	13
α-helix	1128-1135	8
α-helix	1141-1158	18
α-helix	1159-1164	6
α-helix	1166-1177	12
α-helix	1182-1198	17
α-helix	1206-1215	10
α-helix	1216-1219	4
α-helix	1224-1241	18
α-helix	1243-1250	8
α-helix	1260-1274	15
α-helix	1279-1283	5
α-helix	1285-1286	2
α-helix	1299-1301	3

Chain 2: 18 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	451-458	8
α-helix	459-461	3
α-helix	462-468	7
α-helix	479-481	3
α-helix	485-492	8
α-helix	499-501	3
α-helix	502-504	3
α-helix	510-514	5
α-helix	520-525	6
α-helix	526-531	6
α-helix	533-545	13
α-helix	568-575	8
α-helix	585-586	2
α-helix	598-600	3
α-helix	612-617	6
α-helix	625-627	3
α-helix	633-639	7
β-strand	651	1	61
β-strand	655	1	61
α-helix	656-658	3

Chain 3: 18 helices, 104 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	128
β-strand	16-20	5	129
β-strand	29-33	5	129
β-strand	37-43	7	129
β-strand	50-57	8	129
β-strand	62-68	7	130
β-strand	77-82	6	130
β-strand	86-93	8	130
β-strand	98-106	9	130
β-strand	120-123	4	131
β-strand	129-133	5	131
β-strand	139-143	5	131
β-strand	144-146	3	132
β-strand	152-154	3	132
β-strand	166	1	133
β-strand	172-173	2	134
α-helix	174	1
β-strand	181-182	2	134
β-strand	183-184	2	135
β-strand	187	1	133
α-helix	192-194	3
β-strand	207-214	8	135
β-strand	219-227	9	135
β-strand	232-237	6	136
β-strand	248-252	5	136
β-strand	255-259	5	136
α-helix	264-266	3
β-strand	267-270	4	136
α-helix	271-272	2
β-strand	273-274	2	137
β-strand	287-294	8	138
β-strand	299-305	7	138
β-strand	309-318	10	138
β-strand	321-332	12	138
β-strand	336-340	5	139
β-strand	346-351	6	139
β-strand	357-361	5	139
α-helix	371-373	3
β-strand	386-387	2	137
β-strand	395-399	5	139
β-strand	409-413	5	140
α-helix	421	1
β-strand	422-427	6	140
α-helix	430-432	3
β-strand	434-438	5	140
β-strand	440	1	141
β-strand	444-451	8	142
β-strand	456-462	7	143
β-strand	473-478	6	143
β-strand	482-486	5	143
β-strand	492-493	2	143
β-strand	507-510	4	144
β-strand	516-520	5	144
β-strand	523-527	5	144
β-strand	533-536	4	144
β-strand	544-549	6	145
β-strand	553-558	6	145
β-strand	562-568	7	145
β-strand	574-576	3	145
β-strand	581-582	2	145
β-strand	589	1	146
β-strand	592	1	146
α-helix	593-596	4
β-strand	605-608	4	146
β-strand	613-618	6	146
β-strand	626-632	7	146
β-strand	639-644	6	147
β-strand	663-668	6	147
β-strand	674-679	6	147
β-strand	683-689	7	147
β-strand	700-706	7	148
β-strand	709-715	7	148
β-strand	719-724	6	148
β-strand	729-733	5	148
β-strand	739-745	7	142
β-strand	753-758	6	142
β-strand	761-767	7	142
β-strand	774	1	141
β-strand	777-780	4	140
β-strand	784-791	8	149
β-strand	796-803	8	149
α-helix	809-824	16
α-helix	839-844	6
α-helix	849-852	4
β-strand	863-869	7	149
β-strand	878-880	3	149
β-strand	886-894	9	150
β-strand	904-911	8	150
β-strand	914	1	151
β-strand	919	1	151
β-strand	922-928	7	150
β-strand	931	1	152
β-strand	936	1	152
β-strand	939-942	4	150
β-strand	945	1	150
β-strand	949-955	7	153
β-strand	958-963	6	153
β-strand	966-971	6	153
β-strand	978-983	6	153
β-strand	990-995	6	154
β-strand	999-1004	6	154
β-strand	1009-1015	7	154
β-strand	1020-1024	5	154
β-strand	1025	1	155
β-strand	1026	1	154
β-strand	1036-1038	3	156
β-strand	1043-1047	5	156
β-strand	1052-1057	6	156
β-strand	1087	1	155
β-strand	1090-1096	7	156
β-strand	1101-1107	7	128
α-helix	1113-1115	3
β-strand	1116-1121	6	128
β-strand	1126-1131	6	128
α-helix	1135-1151	17
α-helix	1160-1164	5
β-strand	1171	1	156
β-strand	1173-1175	3	128
α-helix	1176-1179	4
α-helix	1182-1184	3
α-helix	1187-1196	10
α-helix	1201-1215	15

Chain 4: 6 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	14-18	5	62
α-helix	26-34	9
β-strand	39-43	5	62
β-strand	46	1	63
β-strand	53	1	63
β-strand	56-61	6	62
α-helix	64-73	10
β-strand	77-79	3	64
β-strand	82-84	3	64
β-strand	85-88	4	62
β-strand	101-105	5	65
α-helix	113-120	8
α-helix	121-123	3
β-strand	126	1	66
β-strand	131-134	4	65
β-strand	141-148	8	65
β-strand	149	1	66
α-helix	152-162	11
β-strand	166	1	67
β-strand	171	1	67
β-strand	173-176	4	65
α-helix	177-179	3

Chain 5: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	2-17	16
α-helix	28-45	18
α-helix	49-56	8
α-helix	61-71	11

Chain 6: 6 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	20-24	5	73
α-helix	26-27	2
α-helix	32-38	7
α-helix	39-42	4
β-strand	45-51	7	73
β-strand	60-64	5	73
α-helix	67-76	10
β-strand	81-82	2	74
β-strand	85-86	2	74
α-helix	87	1
β-strand	88-91	4	73
α-helix	103-116	14

Chain 7: 5 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	18-19	2	75
β-strand	21-22	2	76
β-strand	30	1	77
β-strand	41-42	2	77
β-strand	44-45	2	75
α-helix	48-50	3
α-helix	52-54	3
β-strand	57	1	78
β-strand	58	1	76
β-strand	64	1	78
β-strand	67-68	2	76
β-strand	70-71	2	77
α-helix	73-77	5
α-helix	80-82	3
α-helix	93-105	13

Chain 8: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	20-27	8
α-helix	49-59	11
α-helix	65-75	11
α-helix	82-90	9
α-helix	96-114	19
α-helix	121-125	5

39 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Pre-mRNA-processing-splicing factor 8	A	protein	2335	Homo sapiens	Q6P2Q9 (AlphaFold model)
U5 snRNA	B	RNA	117	Homo sapiens
116 kDa U5 small nuclear ribonucleoprotein component	C	protein	972	Homo sapiens	Q15029 (AlphaFold model)
U5 small nuclear ribonucleoprotein 200 kDa helicase	D	protein	2136	Homo sapiens	O75643 (AlphaFold model)
U5 small nuclear ribonucleoprotein 40 kDa protein	E	protein	357	Homo sapiens	Q96DI7 (AlphaFold model)
U6 snRNA	F	RNA	107	Homo sapiens
pre-mRNA	G	RNA	220	unidentified adenovirus
U2 snRNA	H	RNA	188	Homo sapiens
Pre-mRNA-splicing factor SYF1	I	protein	855	Homo sapiens	Q9HCS7
Crooked neck-like protein 1	J	protein	848	Homo sapiens	Q9BZJ0
DNA/RNA-binding protein KIN17	K	protein	393	Homo sapiens	O60870
Cell division cycle 5-like protein	L	protein	802	Homo sapiens	Q99459

32 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>8I0P_1 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Sequence of entity 2 (B), FASTA

>8I0P_2 U5 snRNA (chains B)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGGCCUUGCUUUGGCAAGGCUA

Sequence of entity 3 (C), FASTA

>8I0P_3 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM

Sequence of entity 4 (D), FASTA

>8I0P_4 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains D)
MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTK
PQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFI
QAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKIT
DYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTL
SANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDR
ECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFL
YQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGS
HFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKT
LNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIY
IAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKG
GERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYED
VATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQ
VLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPY
GFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGR
WTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDM
LNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTA
ALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSL
SSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMAD
MVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVV
KKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKV
ELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFE
PLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESL
YQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYIT
PMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWK
QRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDV
AHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVI
VFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYL
HEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYP
IYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEI
VTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSK
CISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPI
RHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAI
RLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVE
SVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQ
LEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATG
AHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Sequence of entity 5 (E), FASTA

>8I0P_5 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ

Sequence of entity 6 (F), FASTA

>8I0P_6 U6 snRNA (chains F)
GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGC
CCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUUU

Sequence of entity 7 (G), FASTA

>8I0P_7 pre-mRNA (chains G)
GAUCGGGCUCGCCACUCUUGGAUCGGAACCCGUCGGCCUCCGAACGGUAAGAGCCUAGCA
UGUAGAACUGGUUACCUGCAGCCCAAGCUUGCUGCACGUCUAGGGCGCAGUAGUCCAGGG
UUUCCUUGAUGAUGUCAUACUUAUCCUGUCCCUUUUUUUUCCACAGCUCGCGGUUGAGGA
CAAACUCUUCGCGGUCUUUCCAGUGGGGAUCCGAUAUCCG

Sequence of entity 8 (H), FASTA

>8I0P_8 U2 snRNA (chains H)
AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAUGGAUUUUUGGAGCAGGGAGAUGGA
AUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACG
GUGCACCC

Sequence of entity 9 (I), FASTA

>8I0P_9 Pre-mRNA-splicing factor SYF1 (chains I)
MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERA
LKLLPCSYKLWYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFL
MDQGRVTHTRRTFDRALRALPITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESA
EEYIEYLKSSDRLDEAAQRLATVVNDERFVSKAGKSNYQLWHELCDLISQNPDKVQSLNV
DAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYEEAIRTVMTVRDFTQVFDSYA
QFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLLRQNPHHVHEW
HKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEK
ATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRV
YKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKA
YERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYA
QLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLS
DEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTI
KEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMKLLEQRAEQLA
AEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLE
QQSVPAAVFGSLKED

Sequence of entity 10 (J), FASTA

>8I0P_10 Crooked neck-like protein 1 (chains J)
MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCAL
DALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQ
VPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVK
NKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRT
VISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWD
RAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVD
RARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA
KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQY
EEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINY
ALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSI
GKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRAR
AIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFEL
SSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPE
KVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
EDVDESES

Sequence of entity 11 (K), FASTA

>8I0P_11 DNA/RNA-binding protein KIN17 (chains K)
MGKSDFLTPKAIANRIKSKGLQKLRWYCQMCQKQCRDENGFKCHCMSESHQRQLLLASEN
PQQFMDYFSEEFRNDFLELLRRRFGTKRVHNNIVYNEYISHREHIHMNATQWETLTDFTK
WLGREGLCKVDETPKGWYIQYIDRDPETIRRQLELEKKKKQDLDDEEKTAKFIEEQVRRG
LEGKEQEVPTFTELSRENDEEKVTFNLSKGACSSSGATSSKSSTLGPSALKTIGSSASVK
RKESSQSSTQSKEKKKKKSALDEIMEIEEEKKRTARTDYWLQPEIIVKIITKKLGEKYHK
KKAIVKEVIDKYTAVVKMIDSGDKLKLDQTHLETVIPAPGKRILVLNGGYRGNEGTLESI
NEKTFSATIVIETGPLKGRRVEGIQYEDISKLA

Sequence of entity 12 (L), FASTA

>8I0P_12 Cell division cycle 5-like protein (chains L)
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKK
TEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDP
RKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEAR
RLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFR
KLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAP
QISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQD
RILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGA
EGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGL
LGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKA
VQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKG
KTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQV
WEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKIL
LGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQER
EKELQHRYADLLLEKETLKSKF

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	4
MG	Magnesium ion	Mg	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1

Primary citation

Molecular basis for the activation of human spliceosome. Zhan, X., Lu, Y., Shi, Y. Nat Commun (2024) 15:6348-6348. DOI 10.1038/s41467-024-50785-0 · PubMed

Other PDB entries of the same protein (UniProt Q6P2Q9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4JK8 1.15 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
4JKB 1.3 Å, Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
4JKA 1.32 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
4JK7 1.4 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
4JK9 1.5 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
4JKC 1.5 Å, Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
4JKD 1.55 Å, Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
4JKE 1.65 Å, Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
4JKG 1.8 Å, Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain…
4JKH 1.8 Å, Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
3ENB 1.85 Å, Crystal Structure of PRP8 core domain IV
3LRU 1.85 Å, hPRP8 Non-Native Subdomain

Browse structure collections

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