8I0S: Human Bact-II complex

The cryo-EM structure of human Bact-II complex. Determined by electron microscopy at 4.2 Å resolution. Released 31 Jul 2024.

Method: Electron microscopy
Resolution: 4.2 Å
Organisms: Homo sapiens, unidentified adenovirus
Chains: 50
Atoms: 112,874
Mol. weight: 3167.66 kDa
Ligands: ZN, MG, GTP, IHP
Released: 31 Jul 2024

Explore 8I0S in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8I0S contains 657 α-helices and 633 β-strands across 46 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 1: 58 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	490-498	9
α-helix	501-505	5
α-helix	509-526	18
α-helix	528-539	12
α-helix	546-562	17
α-helix	568-570	3
α-helix	571-578	8
α-helix	579-581	3
α-helix	587-603	17
α-helix	607-613	7
α-helix	622-638	17
α-helix	645-653	9
α-helix	658-674	17
α-helix	676-679	4
α-helix	685-690	6
α-helix	699-715	17
α-helix	721-724	4
α-helix	725-727	3
α-helix	728-737	10
α-helix	741-756	16
α-helix	759-762	4
α-helix	764-776	13
α-helix	782-795	14
α-helix	803-809	7
α-helix	811-818	8
α-helix	821-825	5
α-helix	827-844	18
α-helix	849-852	4
α-helix	855-858	4
α-helix	862-879	18
α-helix	886-902	17
α-helix	908-921	14
α-helix	926-928	3
α-helix	929-940	12
β-strand	942	1	13
α-helix	945-964	20
α-helix	968-981	14
α-helix	987-1003	17
α-helix	1006-1008	3
α-helix	1013-1020	8
α-helix	1021-1023	3
α-helix	1029-1041	13
α-helix	1042-1046	5
α-helix	1052-1062	11
α-helix	1071-1087	17
α-helix	1090-1101	12
α-helix	1107-1122	16
α-helix	1129-1135	7
α-helix	1141-1158	18
α-helix	1159-1162	4
α-helix	1166-1177	12
α-helix	1182-1198	17
α-helix	1205-1215	11
α-helix	1216-1219	4
α-helix	1224-1241	18
α-helix	1243-1249	7
α-helix	1260-1274	15
α-helix	1280-1282	3
α-helix	1298-1301	4

Chain 2: 13 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	452-455	4
α-helix	462-468	7
α-helix	478-481	4
α-helix	485-492	8
α-helix	499-501	3
α-helix	520-522	3
α-helix	526-529	4
α-helix	533-546	14
α-helix	549-557	9
α-helix	570-577	8
α-helix	611-615	5
α-helix	624-627	4
α-helix	631-639	9
β-strand	651	1	22
β-strand	655	1	22
β-strand	675	1	23
β-strand	681	1	23

Chain 3: 16 helices, 101 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	139
β-strand	16-20	5	140
β-strand	29-33	5	140
β-strand	37-43	7	140
β-strand	50-57	8	140
β-strand	62-68	7	141
β-strand	77-82	6	141
β-strand	86-93	8	141
β-strand	98-106	9	141
β-strand	120-123	4	142
β-strand	129-133	5	142
β-strand	139-143	5	142
β-strand	144-145	2	143
β-strand	153-154	2	143
β-strand	170-173	4	144
β-strand	181-184	4	144
α-helix	189-192	4
β-strand	207	1	145
β-strand	209-212	4	144
β-strand	221-224	4	144
β-strand	227	1	145
β-strand	235-237	3	146
α-helix	238	1
β-strand	248-250	3	146
β-strand	255	1	147
β-strand	257-259	3	146
α-helix	264-266	3
β-strand	267	1	146
β-strand	270	1	147
α-helix	271-272	2
β-strand	273-274	2	148
β-strand	287-294	8	149
β-strand	299-305	7	149
β-strand	309-317	9	149
β-strand	322-332	11	149
β-strand	336-341	6	150
β-strand	346-351	6	150
β-strand	356-361	6	150
β-strand	386-387	2	148
β-strand	395-401	7	150
β-strand	409	1	151
β-strand	412-413	2	151
α-helix	421	1
β-strand	422-427	6	151
α-helix	430-432	3
β-strand	434-439	6	151
β-strand	445-451	7	152
β-strand	456-462	7	153
β-strand	473-478	6	153
β-strand	482-486	5	153
β-strand	494	1	153
β-strand	507-510	4	154
β-strand	516-519	4	154
β-strand	523-527	5	154
β-strand	534-537	4	154
β-strand	544-549	6	155
β-strand	554-558	5	155
α-helix	559-561	3
β-strand	563	1	155
β-strand	566-568	3	156
β-strand	574-576	3	156
β-strand	592	1	157
α-helix	593-596	4
β-strand	605-609	5	157
β-strand	613-618	6	157
β-strand	626-627	2	157
β-strand	631-632	2	157
β-strand	637-644	8	158
β-strand	663-669	7	158
β-strand	673-678	6	158
β-strand	687-690	4	158
β-strand	700-705	6	159
β-strand	710-715	6	159
β-strand	719-724	6	159
β-strand	729-733	5	159
β-strand	741-745	5	152
β-strand	753-758	6	152
β-strand	761-766	6	152
β-strand	775-780	6	151
β-strand	785-789	5	160
β-strand	797-803	7	160
α-helix	809-824	16
α-helix	839-844	6
α-helix	849-852	4
β-strand	863-869	7	160
β-strand	878-880	3	160
β-strand	886-894	9	161
β-strand	904-911	8	161
β-strand	914	1	162
β-strand	919	1	162
β-strand	923-928	6	161
β-strand	931	1	163
β-strand	936	1	163
β-strand	939-945	7	161
β-strand	951-955	5	164
β-strand	958-963	6	164
β-strand	966-972	7	164
β-strand	977-983	7	164
β-strand	990-995	6	165
β-strand	999-1004	6	165
β-strand	1009-1015	7	165
β-strand	1020-1026	7	165
β-strand	1036-1038	3	166
β-strand	1043-1047	5	166
β-strand	1052-1057	6	166
β-strand	1087-1088	2	165
β-strand	1090-1096	7	166
β-strand	1101-1107	7	139
β-strand	1116-1121	6	139
β-strand	1126-1131	6	139
α-helix	1135-1151	17
α-helix	1160-1164	5
β-strand	1171	1	166
β-strand	1173-1175	3	139
α-helix	1176-1179	4
α-helix	1187-1196	10
α-helix	1201-1213	13

Chain 4: 5 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	14-20	7	32
α-helix	26-36	11
β-strand	39-43	5	32
β-strand	55-61	7	32
α-helix	64-74	11
β-strand	77-79	3	33
β-strand	82-84	3	33
β-strand	85-88	4	32
β-strand	101-105	5	34
α-helix	113-120	8
β-strand	128-134	7	34
β-strand	141-148	8	34
α-helix	152-161	10
β-strand	173-176	4	34
α-helix	177-179	3

Chain 5: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	6-17	12
α-helix	28-45	18
α-helix	49-56	8
α-helix	61-71	11

Chain 7: 3 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	18-19	2	55
α-helix	20	1
β-strand	21	1	56
β-strand	22	1	57
β-strand	39-42	4	58
β-strand	44-45	2	55
α-helix	52-54	3
β-strand	57	1	59
β-strand	58	1	56
β-strand	64	1	59
β-strand	67	1	57
β-strand	70-72	3	58
α-helix	73-77	5

Chain 9: 7 helices, 30 β-strands

Element	Residues	Length	Sheet
β-strand	41	1	72
β-strand	48	1	72
β-strand	54	1	73
β-strand	60	1	73
α-helix	63-72	10
β-strand	75	1	74
β-strand	82	1	74
β-strand	103	1	75
β-strand	108	1	75
β-strand	116-119	4	76
β-strand	127	1	76
α-helix	128-135	8
β-strand	142	1	77
β-strand	149	1	77
β-strand	155-159	5	76
α-helix	202-214	13
α-helix	220-224	5
α-helix	245-250	6
β-strand	280-286	7	78
β-strand	289-295	7	78
α-helix	301-313	13
β-strand	320-321	2	79
β-strand	323-325	3	78
β-strand	329-332	4	78
β-strand	334	1	79
β-strand	350	1	80
β-strand	365-368	4	78
β-strand	376	1	80
β-strand	380-383	4	78
β-strand	391	1	81
β-strand	394	1	81
β-strand	396-402	7	78
α-helix	404-412	9
β-strand	415-416	2	82
β-strand	421-422	2	82
β-strand	426-427	2	79
β-strand	428-433	6	78

Chain a: 3 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
β-strand	16-21	6	83
β-strand	26-33	8	83
β-strand	40-48	9	83
β-strand	64-72	9	83
α-helix	74-76	3
β-strand	77-84	8	83
α-helix	85-87	3

38 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Pre-mRNA-processing-splicing factor 8	A	protein	2335	Homo sapiens	Q6P2Q9 (AlphaFold model)
U5 snRNA	B	RNA	117	Homo sapiens
116 kDa U5 small nuclear ribonucleoprotein component	C	protein	972	Homo sapiens	Q15029 (AlphaFold model)
U5 small nuclear ribonucleoprotein 200 kDa helicase	D	protein	2136	Homo sapiens	O75643 (AlphaFold model)
U5 small nuclear ribonucleoprotein 40 kDa protein	E	protein	357	Homo sapiens	Q96DI7 (AlphaFold model)
U6 snRNA	F	RNA	107	Homo sapiens
pre-mRNA	G	RNA	220	unidentified adenovirus
U2 snRNA	H	RNA	188	Homo sapiens
Pre-mRNA-splicing factor SYF1	I	protein	855	Homo sapiens	Q9HCS7
Crooked neck-like protein 1	J	protein	848	Homo sapiens	Q9BZJ0
RING finger protein 113A	K	protein	343	Homo sapiens	O15541
Cell division cycle 5-like protein	L	protein	802	Homo sapiens	Q99459

31 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>8I0S_1 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Sequence of entity 2 (B), FASTA

>8I0S_2 U5 snRNA (chains B)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGGCCUUGCUUUGGCAAGGCUA

Sequence of entity 3 (C), FASTA

>8I0S_3 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM

Sequence of entity 4 (D), FASTA

>8I0S_4 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains D)
MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTK
PQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFI
QAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKIT
DYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTL
SANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDR
ECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFL
YQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGS
HFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKT
LNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIY
IAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKG
GERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYED
VATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQ
VLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPY
GFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGR
WTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDM
LNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTA
ALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSL
SSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMAD
MVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVV
KKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKV
ELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFE
PLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESL
YQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYIT
PMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWK
QRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDV
AHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVI
VFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYL
HEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYP
IYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEI
VTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSK
CISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPI
RHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAI
RLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVE
SVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQ
LEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATG
AHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Sequence of entity 5 (E), FASTA

>8I0S_5 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ

Sequence of entity 6 (F), FASTA

>8I0S_6 U6 snRNA (chains F)
GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGC
CCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUUU

Sequence of entity 7 (G), FASTA

>8I0S_7 pre-mRNA (chains G)
GAUCGGGCUCGCCACUCUUGGAUCGGAACCCGUCGGCCUCCGAACGGUAAGAGCCUAGCA
UGUAGAACUGGUUACCUGCAGCCCAAGCUUGCUGCACGUCUAGGGCGCAGUAGUCCAGGG
UUUCCUUGAUGAUGUCAUACUUAUCCUGUCCCUUUUUUUUCCACAGCUCGCGGUUGAGGA
CAAACUCUUCGCGGUCUUUCCAGUGGGGAUCCGAUAUCCG

Sequence of entity 8 (H), FASTA

>8I0S_8 U2 snRNA (chains H)
AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAUGGAUUUUUGGAGCAGGGAGAUGGA
AUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACG
GUGCACCC

Sequence of entity 9 (I), FASTA

>8I0S_9 Pre-mRNA-splicing factor SYF1 (chains I)
MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERA
LKLLPCSYKLWYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFL
MDQGRVTHTRRTFDRALRALPITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESA
EEYIEYLKSSDRLDEAAQRLATVVNDERFVSKAGKSNYQLWHELCDLISQNPDKVQSLNV
DAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYEEAIRTVMTVRDFTQVFDSYA
QFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLLRQNPHHVHEW
HKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEK
ATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRV
YKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKA
YERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYA
QLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLS
DEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTI
KEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMKLLEQRAEQLA
AEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLE
QQSVPAAVFGSLKED

Sequence of entity 10 (J), FASTA

>8I0S_10 Crooked neck-like protein 1 (chains J)
MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCAL
DALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQ
VPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVK
NKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRT
VISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWD
RAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVD
RARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA
KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQY
EEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINY
ALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSI
GKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRAR
AIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFEL
SSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPE
KVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
EDVDESES

Sequence of entity 11 (K), FASTA

>8I0S_11 RING finger protein 113A (chains K)
MAEQLSPGKAVDQVCTFLFKKPGRKGAAGRRKRPACDPEPGESGSSSDEGCTVVRPEKKR
VTHNPMIQKTRDSGKQKAAYGDLSSEEEEENEPESLGVVYKSTRSAKPVGPEDMGATAVY
ELDTEKERDAQAIFERSQKIQEELRGKEDDKIYRGINNYQKYMKPKDTSMGNASSGMVRK
GPIRAPEHLRATVRWDYQPDICKDYKETGFCGFGDSCKFLHDRSDYKHGWQIERELDEGR
YGVYEDENYEVGSDDEEIPFKCFICRQSFQNPVVTKCRHYFCESCALQHFRTTPRCYVCD
QQTNGVFNPAKELIAKLEKHRATGEGGASDLPEDPDEDAIPIT

Sequence of entity 12 (L), FASTA

>8I0S_12 Cell division cycle 5-like protein (chains L)
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKK
TEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDP
RKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEAR
RLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFR
KLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAP
QISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQD
RILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGA
EGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGL
LGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKA
VQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKG
KTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQV
WEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKIL
LGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQER
EKELQHRYADLLLEKETLKSKF

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	7
MG	Magnesium ion	Mg	7
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
IHP	Inositol hexakisphosphate	C6 H18 O24 P6	1

Primary citation

Molecular basis for the activation of human spliceosome. Zhan, X., Lu, Y., Shi, Y. Nat Commun (2024) 15:6348-6348. DOI 10.1038/s41467-024-50785-0 · PubMed

Other PDB entries of the same protein (UniProt Q6P2Q9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4JK8 1.15 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
4JKB 1.3 Å, Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
4JKA 1.32 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
4JK7 1.4 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
4JK9 1.5 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
4JKC 1.5 Å, Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
4JKD 1.55 Å, Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
4JKE 1.65 Å, Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
4JKG 1.8 Å, Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain…
4JKH 1.8 Å, Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
3ENB 1.85 Å, Crystal Structure of PRP8 core domain IV
3LRU 1.85 Å, hPRP8 Non-Native Subdomain

Browse structure collections

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