8I0U: Human Bact-IV complex
The cryo-EM structure of human Bact-IV complex. Determined by electron microscopy at 3.3 Å resolution. Released 31 Jul 2024.
- Method
- Electron microscopy
- Resolution
- 3.3 Å
- Organism
- Homo sapiens
- Chains
- 53
- Atoms
- 106,396
- Mol. weight
- 3067.22 kDa
- Ligands
- IHP, GTP, MG, ZN
- Released
- 31 Jul 2024
Explore 8I0U in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8I0U contains 623 α-helices and 571 β-strands across 49 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 1: 57 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 490-498 | 9 | |
| α-helix | 501-505 | 5 | |
| α-helix | 509-526 | 18 | |
| α-helix | 528-539 | 12 | |
| α-helix | 546-562 | 17 | |
| α-helix | 564-566 | 3 | |
| α-helix | 568-570 | 3 | |
| α-helix | 571-578 | 8 | |
| α-helix | 579-581 | 3 | |
| α-helix | 587-603 | 17 | |
| α-helix | 607-618 | 12 | |
| α-helix | 622-638 | 17 | |
| α-helix | 645-653 | 9 | |
| α-helix | 658-674 | 17 | |
| α-helix | 676-679 | 4 | |
| α-helix | 685-690 | 6 | |
| α-helix | 699-715 | 17 | |
| α-helix | 724-727 | 4 | |
| α-helix | 728-737 | 10 | |
| α-helix | 741-756 | 16 | |
| α-helix | 759-762 | 4 | |
| α-helix | 764-776 | 13 | |
| α-helix | 782-795 | 14 | |
| α-helix | 803-809 | 7 | |
| α-helix | 811-818 | 8 | |
| α-helix | 821-825 | 5 | |
| α-helix | 827-844 | 18 | |
| α-helix | 846-853 | 8 | |
| α-helix | 855-858 | 4 | |
| α-helix | 862-879 | 18 | |
| α-helix | 886-902 | 17 | |
| α-helix | 908-921 | 14 | |
| α-helix | 929-940 | 12 | |
| α-helix | 945-964 | 20 | |
| α-helix | 968-981 | 14 | |
| α-helix | 987-1003 | 17 | |
| α-helix | 1006-1008 | 3 | |
| α-helix | 1013-1020 | 8 | |
| α-helix | 1021-1023 | 3 | |
| α-helix | 1029-1041 | 13 | |
| α-helix | 1042-1046 | 5 | |
| α-helix | 1052-1062 | 11 | |
| α-helix | 1071-1087 | 17 | |
| α-helix | 1093-1101 | 9 | |
| α-helix | 1109-1122 | 14 | |
| α-helix | 1129-1135 | 7 | |
| α-helix | 1141-1158 | 18 | |
| α-helix | 1159-1162 | 4 | |
| α-helix | 1166-1177 | 12 | |
| α-helix | 1182-1198 | 17 | |
| α-helix | 1205-1215 | 11 | |
| α-helix | 1216-1219 | 4 | |
| α-helix | 1224-1241 | 18 | |
| α-helix | 1243-1249 | 7 | |
| α-helix | 1260-1274 | 15 | |
| α-helix | 1280-1282 | 3 | |
| α-helix | 1298-1301 | 4 | |
Chain 2: 14 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 451-457 | 7 | |
| α-helix | 462-468 | 7 | |
| α-helix | 478-481 | 4 | |
| α-helix | 485-492 | 8 | |
| α-helix | 499-501 | 3 | |
| α-helix | 520-522 | 3 | |
| α-helix | 526-529 | 4 | |
| α-helix | 533-546 | 14 | |
| α-helix | 549-557 | 9 | |
| α-helix | 570-577 | 8 | |
| α-helix | 595-597 | 3 | |
| α-helix | 611-615 | 5 | |
| α-helix | 624-627 | 4 | |
| α-helix | 631-639 | 9 | |
| β-strand | 651 | 1 | 95 |
| β-strand | 655 | 1 | 95 |
| β-strand | 675 | 1 | 96 |
| β-strand | 681 | 1 | 96 |
Chain 3: 16 helices, 101 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-9 | 7 | 42 |
| β-strand | 16-20 | 5 | 43 |
| β-strand | 29-33 | 5 | 43 |
| β-strand | 37-43 | 7 | 43 |
| β-strand | 50-57 | 8 | 43 |
| β-strand | 62-68 | 7 | 44 |
| β-strand | 77-82 | 6 | 44 |
| β-strand | 86-93 | 8 | 44 |
| β-strand | 98-106 | 9 | 44 |
| β-strand | 120-123 | 4 | 45 |
| β-strand | 129-133 | 5 | 45 |
| β-strand | 139-143 | 5 | 45 |
| β-strand | 144-145 | 2 | 46 |
| β-strand | 153-154 | 2 | 46 |
| β-strand | 170-173 | 4 | 47 |
| β-strand | 181-184 | 4 | 47 |
| α-helix | 189-192 | 4 | |
| β-strand | 207 | 1 | 48 |
| β-strand | 209-212 | 4 | 47 |
| β-strand | 221-224 | 4 | 47 |
| β-strand | 227 | 1 | 48 |
| β-strand | 235-237 | 3 | 49 |
| α-helix | 238 | 1 | |
| β-strand | 248-250 | 3 | 49 |
| β-strand | 255 | 1 | 50 |
| β-strand | 257-259 | 3 | 49 |
| α-helix | 264-266 | 3 | |
| β-strand | 267 | 1 | 49 |
| β-strand | 270 | 1 | 50 |
| α-helix | 271-272 | 2 | |
| β-strand | 273-274 | 2 | 51 |
| β-strand | 287-294 | 8 | 52 |
| β-strand | 299-305 | 7 | 52 |
| β-strand | 309-317 | 9 | 52 |
| β-strand | 322-332 | 11 | 52 |
| β-strand | 336-341 | 6 | 53 |
| β-strand | 346-351 | 6 | 53 |
| β-strand | 356-361 | 6 | 53 |
| β-strand | 386-387 | 2 | 51 |
| β-strand | 395-401 | 7 | 53 |
| β-strand | 409 | 1 | 54 |
| β-strand | 412-413 | 2 | 54 |
| α-helix | 421 | 1 | |
| β-strand | 422-427 | 6 | 54 |
| α-helix | 430-432 | 3 | |
| β-strand | 434-439 | 6 | 54 |
| β-strand | 445-451 | 7 | 55 |
| β-strand | 456-462 | 7 | 56 |
| β-strand | 473-478 | 6 | 56 |
| β-strand | 482-486 | 5 | 56 |
| β-strand | 494 | 1 | 56 |
| β-strand | 507-510 | 4 | 57 |
| β-strand | 516-519 | 4 | 57 |
| β-strand | 523-527 | 5 | 57 |
| β-strand | 534-537 | 4 | 57 |
| β-strand | 544-549 | 6 | 58 |
| β-strand | 554-558 | 5 | 58 |
| α-helix | 559-561 | 3 | |
| β-strand | 563 | 1 | 58 |
| β-strand | 566-568 | 3 | 59 |
| β-strand | 574-576 | 3 | 59 |
| β-strand | 592 | 1 | 60 |
| α-helix | 593-596 | 4 | |
| β-strand | 605-609 | 5 | 60 |
| β-strand | 613-618 | 6 | 60 |
| β-strand | 626-627 | 2 | 60 |
| β-strand | 631-632 | 2 | 60 |
| β-strand | 637-644 | 8 | 61 |
| β-strand | 663-669 | 7 | 61 |
| β-strand | 673-678 | 6 | 61 |
| β-strand | 687-690 | 4 | 61 |
| β-strand | 700-705 | 6 | 62 |
| β-strand | 710-715 | 6 | 62 |
| β-strand | 719-724 | 6 | 62 |
| β-strand | 729-733 | 5 | 62 |
| β-strand | 741-745 | 5 | 55 |
| β-strand | 753-758 | 6 | 55 |
| β-strand | 761-766 | 6 | 55 |
| β-strand | 775-780 | 6 | 54 |
| β-strand | 785-789 | 5 | 63 |
| β-strand | 797-803 | 7 | 63 |
| α-helix | 809-824 | 16 | |
| α-helix | 839-844 | 6 | |
| α-helix | 849-852 | 4 | |
| β-strand | 863-869 | 7 | 63 |
| β-strand | 878-880 | 3 | 63 |
| β-strand | 886-894 | 9 | 64 |
| β-strand | 904-911 | 8 | 64 |
| β-strand | 914 | 1 | 65 |
| β-strand | 919 | 1 | 65 |
| β-strand | 923-928 | 6 | 64 |
| β-strand | 931 | 1 | 66 |
| β-strand | 936 | 1 | 66 |
| β-strand | 939-945 | 7 | 64 |
| β-strand | 951-955 | 5 | 67 |
| β-strand | 958-963 | 6 | 67 |
| β-strand | 966-972 | 7 | 67 |
| β-strand | 977-983 | 7 | 67 |
| β-strand | 990-995 | 6 | 68 |
| β-strand | 999-1004 | 6 | 68 |
| β-strand | 1009-1015 | 7 | 68 |
| β-strand | 1020-1026 | 7 | 68 |
| β-strand | 1036-1038 | 3 | 69 |
| β-strand | 1043-1047 | 5 | 69 |
| β-strand | 1052-1057 | 6 | 69 |
| β-strand | 1087-1088 | 2 | 68 |
| β-strand | 1090-1096 | 7 | 69 |
| β-strand | 1101-1107 | 7 | 42 |
| β-strand | 1116-1121 | 6 | 42 |
| β-strand | 1126-1131 | 6 | 42 |
| α-helix | 1135-1151 | 17 | |
| α-helix | 1160-1164 | 5 | |
| β-strand | 1171 | 1 | 69 |
| β-strand | 1173-1175 | 3 | 42 |
| α-helix | 1176-1179 | 4 | |
| α-helix | 1187-1196 | 10 | |
| α-helix | 1201-1213 | 13 | |
Chain 4: 6 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 14-20 | 7 | 98 |
| α-helix | 26-36 | 11 | |
| β-strand | 39-43 | 5 | 98 |
| β-strand | 55-61 | 7 | 98 |
| α-helix | 64-74 | 11 | |
| β-strand | 101-105 | 5 | 99 |
| α-helix | 113-120 | 8 | |
| α-helix | 121-123 | 3 | |
| β-strand | 128-134 | 7 | 99 |
| β-strand | 141-148 | 8 | 99 |
| α-helix | 152-161 | 10 | |
| β-strand | 173-176 | 4 | 99 |
| α-helix | 177-179 | 3 | |
Chain 5: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-17 | 12 | |
| α-helix | 28-45 | 18 | |
| α-helix | 49-56 | 8 | |
| α-helix | 61-71 | 11 | |
Chain 7: 3 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 18-19 | 2 | 104 |
| α-helix | 20 | 1 | |
| β-strand | 21 | 1 | 105 |
| β-strand | 22 | 1 | 106 |
| β-strand | 39-42 | 4 | 107 |
| β-strand | 44-45 | 2 | 104 |
| α-helix | 52-54 | 3 | |
| β-strand | 57 | 1 | 108 |
| β-strand | 58 | 1 | 105 |
| β-strand | 64 | 1 | 108 |
| β-strand | 67 | 1 | 106 |
| β-strand | 70-72 | 3 | 107 |
| α-helix | 73-77 | 5 | |
Chain a: 3 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-10 | 4 | |
| β-strand | 16-21 | 6 | 15 |
| β-strand | 26-33 | 8 | 15 |
| β-strand | 40-48 | 9 | 15 |
| β-strand | 64-72 | 9 | 15 |
| α-helix | 74-76 | 3 | |
| β-strand | 77-84 | 8 | 15 |
| α-helix | 85-87 | 3 | |
Chain A: 88 helices, 45 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-44 | 18 | |
| α-helix | 47-49 | 3 | |
| α-helix | 63-73 | 11 | |
| α-helix | 84-90 | 7 | |
| α-helix | 91-94 | 4 | |
| α-helix | 95-103 | 9 | |
| β-strand | 115-121 | 7 | 1 |
| β-strand | 127-129 | 3 | 1 |
| β-strand | 134-135 | 2 | 2 |
| α-helix | 137-156 | 20 | |
| α-helix | 161-164 | 4 | |
| α-helix | 174-177 | 4 | |
| α-helix | 178-181 | 4 | |
| β-strand | 220 | 1 | 3 |
| β-strand | 228-229 | 2 | 3 |
| α-helix | 232-241 | 10 | |
| α-helix | 242-245 | 4 | |
| α-helix | 260-269 | 10 | |
| α-helix | 310-315 | 6 | |
| α-helix | 335-337 | 3 | |
| α-helix | 357-358 | 2 | |
| α-helix | 398-403 | 6 | |
| β-strand | 415-416 | 2 | 3 |
| α-helix | 417-418 | 2 | |
| β-strand | 419-420 | 2 | 2 |
| α-helix | 428-431 | 4 | |
| α-helix | 441-459 | 19 | |
| α-helix | 472-477 | 6 | |
| β-strand | 482-488 | 7 | 1 |
| α-helix | 489-510 | 22 | |
| β-strand | 516-518 | 3 | 4 |
| β-strand | 524-526 | 3 | 4 |
| α-helix | 532-537 | 6 | |
| α-helix | 542-566 | 25 | |
| α-helix | 571-583 | 13 | |
| α-helix | 585-588 | 4 | |
| α-helix | 596-598 | 3 | |
| α-helix | 599-616 | 18 | |
| α-helix | 631-660 | 30 | |
| α-helix | 677-692 | 16 | |
| α-helix | 706-721 | 16 | |
| α-helix | 733-763 | 31 | |
| α-helix | 769-797 | 29 | |
| α-helix | 805-819 | 15 | |
| α-helix | 824-828 | 5 | |
| α-helix | 835-847 | 13 | |
| α-helix | 848-852 | 5 | |
| α-helix | 858-872 | 15 | |
| α-helix | 874-887 | 14 | |
| α-helix | 893 | 1 | |
| β-strand | 894-900 | 7 | 5 |
| β-strand | 905-910 | 6 | 5 |
| α-helix | 913-933 | 21 | |
| β-strand | 940 | 1 | 6 |
| α-helix | 948-959 | 12 | |
| β-strand | 973-980 | 8 | 6 |
| α-helix | 990-998 | 9 | |
| α-helix | 1003-1012 | 10 | |
| β-strand | 1016-1019 | 4 | 5 |
| β-strand | 1022-1025 | 4 | 5 |
| α-helix | 1037-1053 | 17 | |
| α-helix | 1055-1061 | 7 | |
| α-helix | 1077-1080 | 4 | |
| β-strand | 1085-1091 | 7 | 6 |
| β-strand | 1094-1101 | 8 | 6 |
| α-helix | 1103-1116 | 14 | |
| α-helix | 1124-1127 | 4 | |
| α-helix | 1144-1157 | 14 | |
| β-strand | 1174-1178 | 5 | 6 |
| β-strand | 1185-1188 | 4 | 7 |
| β-strand | 1192-1198 | 7 | 7 |
| β-strand | 1213-1218 | 6 | 7 |
| β-strand | 1223-1232 | 10 | 7 |
| α-helix | 1234-1249 | 16 | |
| α-helix | 1256-1274 | 19 | |
| α-helix | 1277-1280 | 4 | |
| α-helix | 1282-1302 | 21 | |
| α-helix | 1314-1317 | 4 | |
| α-helix | 1334 | 1 | |
| β-strand | 1335-1337 | 3 | 8 |
| α-helix | 1340-1343 | 4 | |
| β-strand | 1352-1354 | 3 | 8 |
| α-helix | 1368-1370 | 3 | |
| α-helix | 1373-1374 | 2 | |
| α-helix | 1375-1398 | 24 | |
| α-helix | 1402-1404 | 3 | |
| α-helix | 1419-1421 | 3 | |
| α-helix | 1436-1442 | 7 | |
| α-helix | 1443-1445 | 3 | |
| α-helix | 1458-1461 | 4 | |
| α-helix | 1468-1478 | 11 | |
| α-helix | 1480-1485 | 6 | |
| α-helix | 1491-1493 | 3 | |
| β-strand | 1501-1503 | 3 | 9 |
| α-helix | 1529-1537 | 9 | |
| α-helix | 1539-1542 | 4 | |
| β-strand | 1553-1554 | 2 | 10 |
| β-strand | 1561-1562 | 2 | 10 |
| α-helix | 1567-1576 | 10 | |
| α-helix | 1581-1604 | 24 | |
| β-strand | 1606-1611 | 6 | 11 |
| α-helix | 1613-1615 | 3 | |
| β-strand | 1629-1639 | 11 | 11 |
| β-strand | 1643-1644 | 2 | 11 |
| β-strand | 1655-1667 | 13 | 11 |
| α-helix | 1676-1689 | 14 | |
| β-strand | 1700-1706 | 7 | 11 |
| β-strand | 1711-1716 | 6 | 11 |
| α-helix | 1722-1736 | 15 | |
| α-helix | 1738-1751 | 14 | |
| β-strand | 1753-1755 | 3 | 9 |
| α-helix | 1768-1771 | 4 | |
| β-strand | 1777-1781 | 5 | 12 |
| β-strand | 1787-1789 | 3 | 13 |
| β-strand | 1792 | 1 | 14 |
| β-strand | 1798 | 1 | 14 |
| β-strand | 1801-1803 | 3 | 13 |
| β-strand | 1805-1811 | 7 | 12 |
| β-strand | 1816-1822 | 7 | 12 |
| α-helix | 1823 | 1 | |
| α-helix | 1824-1826 | 3 | |
| α-helix | 1833-1851 | 19 | |
| β-strand | 1860-1863 | 4 | 12 |
| α-helix | 1869-1875 | 7 | |
| β-strand | 1883-1886 | 4 | 12 |
| α-helix | 1893-1897 | 5 | |
| α-helix | 1900-1908 | 9 | |
| β-strand | 1913-1918 | 6 | 12 |
| α-helix | 1923-1926 | 4 | |
| α-helix | 1929-1945 | 17 | |
| α-helix | 1949-1954 | 6 | |
| α-helix | 1973-1995 | 23 | |
| α-helix | 2004-2012 | 9 | |
41 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Pre-mRNA-processing-splicing factor 8 | A | protein | 2335 | Homo sapiens | Q6P2Q9 (AlphaFold model) |
| U5 snRNA | B | RNA | 117 | Homo sapiens | |
| 116 kDa U5 small nuclear ribonucleoprotein component | C | protein | 972 | Homo sapiens | Q15029 (AlphaFold model) |
| U5 small nuclear ribonucleoprotein 40 kDa protein | E | protein | 357 | Homo sapiens | Q96DI7 (AlphaFold model) |
| U6 snRNA | F | RNA | 107 | Homo sapiens | |
| Pre-mRNA | G | RNA | 220 | Homo sapiens | |
| U2 snRNA | H | RNA | 188 | Homo sapiens | |
| Pre-mRNA-splicing factor SYF1 | I | protein | 855 | Homo sapiens | Q9HCS7 (AlphaFold model) |
| Crooked neck-like protein 1 | J | protein | 848 | Homo sapiens | Q9BZJ0 |
| RING finger protein 113A | K | protein | 343 | Homo sapiens | O15541 |
| Cell division cycle 5-like protein | L | protein | 802 | Homo sapiens | Q99459 |
| Pre-mRNA-splicing factor SYF2 | M | protein | 243 | Homo sapiens | O95926 |
31 more molecules are not listed.
Sequence of entity 1 (A), FASTA
>8I0U_1 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA
Sequence of entity 2 (B), FASTA
>8I0U_2 U5 snRNA (chains B)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGGCCUUGCUUUGGCAAGGCUA
Sequence of entity 3 (C), FASTA
>8I0U_3 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM
Sequence of entity 4 (E), FASTA
>8I0U_4 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ
Sequence of entity 5 (F), FASTA
>8I0U_5 U6 snRNA (chains F)
GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGC
CCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUUU
Sequence of entity 6 (G), FASTA
>8I0U_6 Pre-mRNA (chains G)
GAUCGGGCUCGCCACUCUUGGAUCGGAACCCGUCGGCCUCCGAACGGUAAGAGCCUAGCA
UGUAGAACUGGUUACCUGCAGCCCAAGCUUGCUGCACGUCUAGGGCGCAGUAGUCCAGGG
UUUCCUUGAUGAUGUCAUACUUAUCCUGUCCCUUUUUUUUCCACAGCUCGCGGUUGAGGA
CAAACUCUUCGCGGUCUUUCCAGUGGGGAUCCGAUAUCCG
Sequence of entity 7 (H), FASTA
>8I0U_7 U2 snRNA (chains H)
AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAUGGAUUUUUGGAGCAGGGAGAUGGA
AUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACG
GUGCACCC
Sequence of entity 8 (I), FASTA
>8I0U_8 Pre-mRNA-splicing factor SYF1 (chains I)
MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERA
LKLLPCSYKLWYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFL
MDQGRVTHTRRTFDRALRALPITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESA
EEYIEYLKSSDRLDEAAQRLATVVNDERFVSKAGKSNYQLWHELCDLISQNPDKVQSLNV
DAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYEEAIRTVMTVRDFTQVFDSYA
QFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLLRQNPHHVHEW
HKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEK
ATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRV
YKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKA
YERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYA
QLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLS
DEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTI
KEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMKLLEQRAEQLA
AEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLE
QQSVPAAVFGSLKED
Sequence of entity 9 (J), FASTA
>8I0U_9 Crooked neck-like protein 1 (chains J)
MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCAL
DALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQ
VPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVK
NKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRT
VISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWD
RAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVD
RARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA
KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQY
EEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINY
ALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSI
GKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRAR
AIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFEL
SSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPE
KVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
EDVDESES
Sequence of entity 10 (K), FASTA
>8I0U_10 RING finger protein 113A (chains K)
MAEQLSPGKAVDQVCTFLFKKPGRKGAAGRRKRPACDPEPGESGSSSDEGCTVVRPEKKR
VTHNPMIQKTRDSGKQKAAYGDLSSEEEEENEPESLGVVYKSTRSAKPVGPEDMGATAVY
ELDTEKERDAQAIFERSQKIQEELRGKEDDKIYRGINNYQKYMKPKDTSMGNASSGMVRK
GPIRAPEHLRATVRWDYQPDICKDYKETGFCGFGDSCKFLHDRSDYKHGWQIERELDEGR
YGVYEDENYEVGSDDEEIPFKCFICRQSFQNPVVTKCRHYFCESCALQHFRTTPRCYVCD
QQTNGVFNPAKELIAKLEKHRATGEGGASDLPEDPDEDAIPIT
Sequence of entity 11 (L), FASTA
>8I0U_11 Cell division cycle 5-like protein (chains L)
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKK
TEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDP
RKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEAR
RLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFR
KLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAP
QISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQD
RILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGA
EGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGL
LGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKA
VQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKG
KTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQV
WEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKIL
LGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQER
EKELQHRYADLLLEKETLKSKF
Sequence of entity 12 (M), FASTA
>8I0U_12 Pre-mRNA-splicing factor SYF2 (chains M)
MAAIAASEVLVDSAEEGSLAAAAELAAQKREQRLRKFRELHLMRNEARKLNHQEVVEEDK
RLKLPANWEAKKARLEWELKEEEKKKECAARGEDYEKVKLLEISAEDAERWERKKKRKNP
DLGFSDYAAAQLRQYHRLTKQIKPDMETYERLREKHGEEFFPTSNSLLHGTHVPSTEEID
RMVIDLEKQIEKRDKYSRRRPYNDDADIDYINERNAKFNKKAERFYGKYTAEIKQNLERG
TAV
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| IHP | Inositol hexakisphosphate | C6 H18 O24 P6 | 1 |
| GTP | Guanosine-5'-triphosphate | C10 H16 N5 O14 P3 | 1 |
| MG | Magnesium ion | Mg | 7 |
| ZN | Zinc ion | Zn | 7 |
Primary citation
Molecular basis for the activation of human spliceosome. Zhan, X., Lu, Y., Shi, Y. Nat Commun (2024) 15:6348-6348. DOI 10.1038/s41467-024-50785-0 · PubMed
Other PDB entries of the same protein (UniProt Q6P2Q9 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 4JK8 1.15 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
- 4JKB 1.3 Å, Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
- 4JKA 1.32 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
- 4JK7 1.4 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
- 4JK9 1.5 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
- 4JKC 1.5 Å, Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
- 4JKD 1.55 Å, Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
- 4JKE 1.65 Å, Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
- 4JKG 1.8 Å, Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain…
- 4JKH 1.8 Å, Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
- 3ENB 1.85 Å, Crystal Structure of PRP8 core domain IV
- 3LRU 1.85 Å, hPRP8 Non-Native Subdomain
Browse structure collections
About this viewer
MolViewer shows 8I0U directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.