8I0W: Human C complex
The cryo-EM structure of human C complex. Determined by electron microscopy at 3.4 Å resolution. Released 31 Jul 2024.
- Method
- Electron microscopy
- Resolution
- 3.4 Å
- Organisms
- Homo sapiens, unidentified adenovirus
- Chains
- 58
- Atoms
- 106,256
- Mol. weight
- 3241.21 kDa
- Ligands
- IHP, GTP, MG, ZN
- Released
- 31 Jul 2024
Explore 8I0W in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8I0W contains 687 α-helices and 619 β-strands across 52 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 1: 7 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 176-179 | 4 | 137 |
| α-helix | 184 | 1 | |
| β-strand | 185-189 | 5 | 138 |
| β-strand | 197-201 | 5 | 138 |
| β-strand | 206-209 | 4 | 138 |
| β-strand | 222-224 | 3 | 138 |
| α-helix | 230-231 | 2 | |
| β-strand | 232-234 | 3 | 139 |
| β-strand | 237 | 1 | 139 |
| β-strand | 244-248 | 5 | 139 |
| β-strand | 253-256 | 4 | 139 |
| β-strand | 263 | 1 | 139 |
| α-helix | 265-267 | 3 | |
| α-helix | 276-278 | 3 | |
| β-strand | 286-291 | 6 | 140 |
| α-helix | 292 | 1 | |
| β-strand | 299-303 | 5 | 140 |
| β-strand | 307-311 | 5 | 140 |
| β-strand | 321-323 | 3 | 140 |
| β-strand | 326 | 1 | 141 |
| β-strand | 332 | 1 | 141 |
| β-strand | 335-340 | 6 | 142 |
| β-strand | 347-351 | 5 | 142 |
| β-strand | 356-359 | 4 | 142 |
| β-strand | 384-386 | 3 | 143 |
| β-strand | 393-395 | 3 | 143 |
| β-strand | 403-405 | 3 | 143 |
| β-strand | 413 | 1 | 143 |
| β-strand | 429-430 | 2 | 144 |
| β-strand | 437-441 | 5 | 144 |
| β-strand | 452-457 | 6 | 144 |
| β-strand | 465-468 | 4 | 144 |
| β-strand | 476-479 | 4 | 137 |
| β-strand | 486-490 | 5 | 137 |
| β-strand | 494-497 | 4 | 137 |
| α-helix | 583-594 | 12 | |
| α-helix | 602-612 | 11 | |
Chain 2: 7 helices, 19 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 48-49 | 2 | 154 |
| α-helix | 53-55 | 3 | |
| β-strand | 58-65 | 8 | 154 |
| β-strand | 68-69 | 2 | 154 |
| β-strand | 70-72 | 3 | 155 |
| β-strand | 77 | 1 | 154 |
| β-strand | 78-80 | 3 | 155 |
| α-helix | 81-84 | 4 | |
| α-helix | 89-92 | 4 | |
| α-helix | 93-95 | 3 | |
| β-strand | 97-101 | 5 | 154 |
| β-strand | 107-110 | 4 | 154 |
| β-strand | 116-119 | 4 | 154 |
| β-strand | 124 | 1 | 156 |
| β-strand | 125-129 | 5 | 154 |
| β-strand | 137-138 | 2 | 154 |
| β-strand | 139-143 | 5 | 156 |
| β-strand | 146-150 | 5 | 156 |
| β-strand | 156-159 | 4 | 156 |
| β-strand | 164 | 1 | 155 |
| β-strand | 165-169 | 5 | 156 |
| α-helix | 174-176 | 3 | |
| β-strand | 178 | 1 | 156 |
| β-strand | 179-182 | 4 | 154 |
| α-helix | 232-237 | 6 | |
| α-helix | 240-248 | 9 | |
Chain 3: 5 helices, 42 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 79-86 | 8 | 125 |
| β-strand | 92-97 | 6 | 126 |
| β-strand | 103-108 | 6 | 126 |
| β-strand | 112-117 | 6 | 126 |
| β-strand | 125-130 | 6 | 126 |
| β-strand | 136-141 | 6 | 127 |
| β-strand | 147-152 | 6 | 127 |
| β-strand | 157-161 | 5 | 127 |
| β-strand | 166-171 | 6 | 127 |
| β-strand | 183-188 | 6 | 128 |
| β-strand | 193-198 | 6 | 128 |
| β-strand | 203-207 | 5 | 128 |
| β-strand | 215-217 | 3 | 128 |
| α-helix | 225 | 1 | |
| β-strand | 226-231 | 6 | 129 |
| β-strand | 238-242 | 5 | 129 |
| β-strand | 247-250 | 4 | 129 |
| β-strand | 264-266 | 3 | 129 |
| α-helix | 282 | 1 | |
| β-strand | 283-288 | 6 | 130 |
| β-strand | 294-299 | 6 | 130 |
| β-strand | 303-308 | 6 | 130 |
| β-strand | 313-318 | 6 | 130 |
| β-strand | 360-363 | 4 | 131 |
| β-strand | 369-373 | 5 | 131 |
| β-strand | 378-382 | 5 | 131 |
| β-strand | 388-391 | 4 | 131 |
| β-strand | 398-403 | 6 | 125 |
| β-strand | 438-442 | 5 | 125 |
| β-strand | 448-454 | 7 | 125 |
| β-strand | 493-498 | 6 | 132 |
| β-strand | 501-507 | 7 | 132 |
| α-helix | 513-524 | 12 | |
| β-strand | 532 | 1 | 133 |
| β-strand | 535-537 | 3 | 132 |
| β-strand | 541-544 | 4 | 132 |
| β-strand | 562 | 1 | 134 |
| β-strand | 577-580 | 4 | 132 |
| β-strand | 588 | 1 | 134 |
| β-strand | 592-595 | 4 | 132 |
| α-helix | 600-602 | 3 | |
| β-strand | 608-614 | 7 | 132 |
| α-helix | 616-623 | 8 | |
| β-strand | 627-628 | 2 | 135 |
| β-strand | 633-634 | 2 | 135 |
| β-strand | 639 | 1 | 133 |
| β-strand | 640-645 | 6 | 132 |
Chain 4: 0 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| β-strand | 110-111 | 2 | 16 |
Chain 5: 3 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-12 | 4 | |
| α-helix | 15-20 | 6 | |
| β-strand | 27 | 1 | 12 |
| α-helix | 31-37 | 7 | |
Chain a: 1 helix, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 16-21 | 6 | 75 |
| β-strand | 26-33 | 8 | 75 |
| β-strand | 40-48 | 9 | 75 |
| β-strand | 64-72 | 9 | 75 |
| β-strand | 77-84 | 8 | 75 |
| α-helix | 85-87 | 3 | |
Chain A: 116 helices, 63 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-44 | 18 | |
| α-helix | 47-49 | 3 | |
| α-helix | 64-73 | 10 | |
| α-helix | 84-91 | 8 | |
| α-helix | 92-94 | 3 | |
| α-helix | 95-103 | 9 | |
| β-strand | 115-121 | 7 | 1 |
| β-strand | 127-129 | 3 | 1 |
| β-strand | 134-135 | 2 | 2 |
| α-helix | 137-157 | 21 | |
| α-helix | 162-164 | 3 | |
| α-helix | 174-177 | 4 | |
| α-helix | 178-182 | 5 | |
| α-helix | 201-204 | 4 | |
| β-strand | 228-229 | 2 | 3 |
| α-helix | 232-241 | 10 | |
| α-helix | 242-245 | 4 | |
| α-helix | 260-269 | 10 | |
| α-helix | 310-315 | 6 | |
| α-helix | 334-336 | 3 | |
| α-helix | 398-404 | 7 | |
| α-helix | 409-412 | 4 | |
| β-strand | 415-416 | 2 | 3 |
| β-strand | 419-420 | 2 | 2 |
| α-helix | 421-423 | 3 | |
| α-helix | 428-431 | 4 | |
| α-helix | 441-460 | 20 | |
| α-helix | 473-477 | 5 | |
| β-strand | 482-488 | 7 | 1 |
| α-helix | 489-511 | 23 | |
| β-strand | 516-518 | 3 | 4 |
| β-strand | 524-526 | 3 | 4 |
| α-helix | 529-531 | 3 | |
| α-helix | 532-538 | 7 | |
| α-helix | 542-566 | 25 | |
| α-helix | 571-583 | 13 | |
| α-helix | 585-588 | 4 | |
| α-helix | 591-594 | 4 | |
| α-helix | 596-598 | 3 | |
| α-helix | 599-616 | 18 | |
| α-helix | 631-661 | 31 | |
| α-helix | 670-672 | 3 | |
| α-helix | 674-676 | 3 | |
| α-helix | 677-696 | 20 | |
| α-helix | 703-705 | 3 | |
| α-helix | 706-721 | 16 | |
| α-helix | 733-763 | 31 | |
| α-helix | 769-797 | 29 | |
| α-helix | 803-820 | 18 | |
| α-helix | 822-824 | 3 | |
| β-strand | 825 | 1 | 5 |
| α-helix | 826-827 | 2 | |
| α-helix | 835-847 | 13 | |
| α-helix | 848-850 | 3 | |
| α-helix | 858-872 | 15 | |
| α-helix | 874-886 | 13 | |
| β-strand | 894-900 | 7 | 6 |
| β-strand | 905-910 | 6 | 6 |
| α-helix | 913-933 | 21 | |
| β-strand | 940 | 1 | 7 |
| α-helix | 948-961 | 14 | |
| β-strand | 973-980 | 8 | 7 |
| α-helix | 990-1000 | 11 | |
| β-strand | 1001 | 1 | 5 |
| α-helix | 1003-1012 | 10 | |
| β-strand | 1016-1018 | 3 | 6 |
| β-strand | 1023-1025 | 3 | 6 |
| α-helix | 1037-1053 | 17 | |
| α-helix | 1055-1062 | 8 | |
| α-helix | 1077-1081 | 5 | |
| β-strand | 1085-1091 | 7 | 7 |
| β-strand | 1094-1101 | 8 | 7 |
| α-helix | 1103-1116 | 14 | |
| α-helix | 1123-1125 | 3 | |
| α-helix | 1140-1141 | 2 | |
| α-helix | 1144-1158 | 15 | |
| β-strand | 1174-1178 | 5 | 7 |
| β-strand | 1179 | 1 | 8 |
| β-strand | 1182 | 1 | 8 |
| β-strand | 1185-1188 | 4 | 9 |
| β-strand | 1192 | 1 | 10 |
| β-strand | 1193-1198 | 6 | 9 |
| β-strand | 1213-1217 | 5 | 9 |
| β-strand | 1224-1229 | 6 | 9 |
| β-strand | 1232 | 1 | 10 |
| α-helix | 1234-1250 | 17 | |
| α-helix | 1256-1274 | 19 | |
| α-helix | 1277-1280 | 4 | |
| α-helix | 1282-1302 | 21 | |
| α-helix | 1308-1310 | 3 | |
| α-helix | 1313-1317 | 5 | |
| α-helix | 1334 | 1 | |
| β-strand | 1335-1337 | 3 | 11 |
| α-helix | 1340-1346 | 7 | |
| β-strand | 1352-1354 | 3 | 11 |
| α-helix | 1368-1371 | 4 | |
| α-helix | 1375-1398 | 24 | |
| α-helix | 1409-1411 | 3 | |
| α-helix | 1419-1423 | 5 | |
| α-helix | 1427-1430 | 4 | |
| α-helix | 1436-1446 | 11 | |
| α-helix | 1450-1451 | 2 | |
| α-helix | 1458-1461 | 4 | |
| α-helix | 1468-1478 | 11 | |
| α-helix | 1480-1486 | 7 | |
| α-helix | 1490-1493 | 4 | |
| α-helix | 1498-1500 | 3 | |
| β-strand | 1501-1502 | 2 | 12 |
| α-helix | 1508-1516 | 9 | |
| α-helix | 1523-1525 | 3 | |
| α-helix | 1531-1537 | 7 | |
| α-helix | 1539-1542 | 4 | |
| β-strand | 1552-1554 | 3 | 13 |
| β-strand | 1561-1563 | 3 | 13 |
| α-helix | 1567-1576 | 10 | |
| α-helix | 1581-1598 | 18 | |
| α-helix | 1601-1604 | 4 | |
| β-strand | 1606-1611 | 6 | 14 |
| β-strand | 1629-1639 | 11 | 14 |
| α-helix | 1640-1642 | 3 | |
| β-strand | 1643-1644 | 2 | 14 |
| α-helix | 1652-1654 | 3 | |
| β-strand | 1655-1667 | 13 | 14 |
| α-helix | 1676-1689 | 14 | |
| β-strand | 1700-1706 | 7 | 14 |
| β-strand | 1711-1716 | 6 | 14 |
| α-helix | 1722-1736 | 15 | |
| α-helix | 1738-1751 | 14 | |
| β-strand | 1754-1755 | 2 | 12 |
| α-helix | 1765-1768 | 4 | |
| β-strand | 1777-1781 | 5 | 15 |
| α-helix | 1783-1785 | 3 | |
| β-strand | 1787-1792 | 6 | 16 |
| β-strand | 1798-1803 | 6 | 16 |
| β-strand | 1805-1810 | 6 | 15 |
| β-strand | 1816-1822 | 7 | 15 |
| α-helix | 1824-1827 | 4 | |
| α-helix | 1833-1851 | 19 | |
| β-strand | 1860-1863 | 4 | 15 |
| α-helix | 1866-1868 | 3 | |
| α-helix | 1869-1875 | 7 | |
| β-strand | 1883-1886 | 4 | 15 |
| α-helix | 1894-1898 | 5 | |
| α-helix | 1900-1908 | 9 | |
| β-strand | 1913-1918 | 6 | 15 |
| α-helix | 1923-1925 | 3 | |
| α-helix | 1929-1945 | 17 | |
| α-helix | 1948-1954 | 7 | |
| α-helix | 1973-1995 | 23 | |
| α-helix | 1999-2001 | 3 | |
| α-helix | 2004-2012 | 9 | |
| α-helix | 2071-2080 | 10 | |
| α-helix | 2083-2086 | 4 | |
| β-strand | 2090-2092 | 3 | 17 |
| β-strand | 2103-2107 | 5 | 17 |
| α-helix | 2108-2117 | 10 | |
| β-strand | 2125-2131 | 7 | 17 |
| α-helix | 2132-2133 | 2 | |
| β-strand | 2139-2146 | 8 | 17 |
| β-strand | 2150 | 1 | 18 |
| β-strand | 2151-2152 | 2 | 19 |
| β-strand | 2157-2158 | 2 | 19 |
| α-helix | 2163-2164 | 2 | |
| α-helix | 2167-2170 | 4 | |
| β-strand | 2173-2181 | 9 | 17 |
| α-helix | 2190-2202 | 13 | |
| β-strand | 2212-2219 | 8 | 17 |
| β-strand | 2222-2230 | 9 | 17 |
| α-helix | 2232-2240 | 9 | |
| α-helix | 2253-2255 | 3 | |
| β-strand | 2256-2258 | 3 | 17 |
| β-strand | 2260-2264 | 5 | 17 |
| β-strand | 2270-2274 | 5 | 17 |
| β-strand | 2280 | 1 | 18 |
| β-strand | 2296-2298 | 3 | 17 |
| α-helix | 2300-2303 | 4 | |
| α-helix | 2307-2309 | 3 | |
| α-helix | 2311-2314 | 4 | |
| α-helix | 2321-2325 | 5 | |
Chain b: 3 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-8 | 6 | |
| β-strand | 14-19 | 6 | 28 |
| β-strand | 24-29 | 6 | 28 |
| β-strand | 40-46 | 7 | 28 |
| β-strand | 52-55 | 4 | 28 |
| β-strand | 58-60 | 3 | 75 |
| α-helix | 62-64 | 3 | |
| β-strand | 65-70 | 6 | 28 |
| α-helix | 76-79 | 4 | |
44 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Pre-mRNA-processing-splicing factor 8 | A | protein | 2335 | Homo sapiens | Q6P2Q9 (AlphaFold model) |
| U5 snRNA | B | RNA | 117 | Homo sapiens | |
| 116 kDa U5 small nuclear ribonucleoprotein component | C | protein | 972 | Homo sapiens | Q15029 (AlphaFold model) |
| U5 small nuclear ribonucleoprotein 200 kDa helicase | D | protein | 2136 | Homo sapiens | O75643 (AlphaFold model) |
| U5 small nuclear ribonucleoprotein 40 kDa protein | E | protein | 357 | Homo sapiens | Q96DI7 (AlphaFold model) |
| U6 snRNA | F | RNA | 107 | Homo sapiens | |
| pre-mRNA | G | RNA | 46 | unidentified adenovirus | |
| pre-mRNA | 6 | RNA | 174 | unidentified adenovirus | |
| U2 snRNA | H | RNA | 188 | Homo sapiens | |
| Pre-mRNA-splicing factor SYF1 | I | protein | 855 | Homo sapiens | Q9HCS7 |
| Crooked neck-like protein 1 | J | protein | 848 | Homo sapiens | Q9BZJ0 |
| Pre-mRNA-splicing factor SPF27 | K | protein | 225 | Homo sapiens | O75934 |
36 more molecules are not listed.
Sequence of entity 1 (A), FASTA
>8I0W_1 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA
Sequence of entity 2 (B), FASTA
>8I0W_2 U5 snRNA (chains B)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGGCCUUGCUUUGGCAAGGCUA
Sequence of entity 3 (C), FASTA
>8I0W_3 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM
Sequence of entity 4 (D), FASTA
>8I0W_4 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains D)
MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTK
PQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFI
QAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKIT
DYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTL
SANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDR
ECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFL
YQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGS
HFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKT
LNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIY
IAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKG
GERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYED
VATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQ
VLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPY
GFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGR
WTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDM
LNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTA
ALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSL
SSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMAD
MVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVV
KKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKV
ELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFE
PLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESL
YQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYIT
PMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWK
QRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDV
AHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVI
VFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYL
HEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYP
IYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEI
VTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSK
CISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPI
RHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAI
RLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVE
SVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQ
LEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATG
AHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD
Sequence of entity 5 (E), FASTA
>8I0W_5 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ
Sequence of entity 6 (F), FASTA
>8I0W_6 U6 snRNA (chains F)
GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGC
CCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUUU
Sequence of entity 7 (G), FASTA
>8I0W_7 pre-mRNA (chains G)
GAUCGGGCUCGCCACUCUUGGAUCGGAACCCGUCGGCCUCCGAACG
Sequence of entity 8 (6), FASTA
>8I0W_8 pre-mRNA (chains 6)
GUAAGAGCCUAGCAUGUAGAACUGGUUACCUGCAGCCCAAGCUUGCUGCACGUCUAGGGC
GCAGUAGUCCAGGGUUUCCUUGAUGAUGUCAUACUUAUCCUGUCCCUUUUUUUUCCACAG
CUCGCGGUUGAGGACAAACUCUUCGCGGUCUUUCCAGUGGGGAUCCGAUAUCCG
Sequence of entity 9 (H), FASTA
>8I0W_9 U2 snRNA (chains H)
AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAUGGAUUUUUGGAGCAGGGAGAUGGA
AUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACG
GUGCACCC
Sequence of entity 10 (I), FASTA
>8I0W_10 Pre-mRNA-splicing factor SYF1 (chains I)
MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERA
LKLLPCSYKLWYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFL
MDQGRVTHTRRTFDRALRALPITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESA
EEYIEYLKSSDRLDEAAQRLATVVNDERFVSKAGKSNYQLWHELCDLISQNPDKVQSLNV
DAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYEEAIRTVMTVRDFTQVFDSYA
QFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLLRQNPHHVHEW
HKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEK
ATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRV
YKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKA
YERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYA
QLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLS
DEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTI
KEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMKLLEQRAEQLA
AEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLE
QQSVPAAVFGSLKED
Sequence of entity 11 (J), FASTA
>8I0W_11 Crooked neck-like protein 1 (chains J)
MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCAL
DALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQ
VPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVK
NKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRT
VISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWD
RAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVD
RARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA
KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQY
EEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINY
ALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSI
GKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRAR
AIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFEL
SSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPE
KVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
EDVDESES
Sequence of entity 12 (K), FASTA
>8I0W_12 Pre-mRNA-splicing factor SPF27 (chains K)
MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSA
FETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVR
IENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLRE
MESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| IHP | Inositol hexakisphosphate | C6 H18 O24 P6 | 1 |
| GTP | Guanosine-5'-triphosphate | C10 H16 N5 O14 P3 | 1 |
| MG | Magnesium ion | Mg | 5 |
| ZN | Zinc ion | Zn | 7 |
Primary citation
Molecular basis for the activation of human spliceosome. Zhan, X., Lu, Y., Shi, Y. Nat Commun (2024) 15:6348-6348. DOI 10.1038/s41467-024-50785-0 · PubMed
Other PDB entries of the same protein (UniProt Q6P2Q9 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 4JK8 1.15 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
- 4JKB 1.3 Å, Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
- 4JKA 1.32 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
- 4JK7 1.4 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
- 4JK9 1.5 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
- 4JKC 1.5 Å, Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
- 4JKD 1.55 Å, Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
- 4JKE 1.65 Å, Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
- 4JKG 1.8 Å, Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain…
- 4JKH 1.8 Å, Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
- 3ENB 1.85 Å, Crystal Structure of PRP8 core domain IV
- 3LRU 1.85 Å, hPRP8 Non-Native Subdomain
Browse structure collections
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