8JHI: FZD3-Gs complex

FZD3-Gs complex. Determined by electron microscopy at 3.2 Å resolution. Released 17 Jan 2024.

Method: Electron microscopy
Resolution: 3.2 Å
Organism: Homo sapiens
Chains: 5
Atoms: 8,562
Mol. weight: 145.98 kDa
Released: 17 Jan 2024

Explore 8JHI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8JHI contains 31 α-helices and 52 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	2-34	33
β-strand	36-42	7	1
β-strand	209-214	6	1
β-strand	217-222	6	1
α-helix	234-237	4
β-strand	243-247	5	1
β-strand	249	1	2
α-helix	264-277	14
β-strand	287-289	3	1
β-strand	292	1	2
α-helix	294-303	10
α-helix	308-310	3
α-helix	313-315	3
α-helix	332-349	18
β-strand	359	1	1
α-helix	371-390	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-25	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-52	6	3
β-strand	58-62	5	4
β-strand	71-74	4	4
β-strand	78-81	4	4
β-strand	91-94	4	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	135-139	5	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	176-181	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	275-278	4	9
β-strand	284-288	5	9
β-strand	294-298	5	9
β-strand	304-308	5	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	335-339	5	3

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	32-44	13
α-helix	54-67	14

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	26-30	5	10
β-strand	34	1	11
β-strand	41-48	8	10
α-helix	52-54	3
β-strand	57-62	6	12
β-strand	68-74	7	12
β-strand	81-83	3	12
α-helix	85-87	3
β-strand	92-96	5	10
β-strand	101-106	6	10
α-helix	111-113	3
β-strand	115-121	7	12
β-strand	141	1	12
β-strand	145-147	3	12
β-strand	148	1	11

Chain R: 15 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	179	1	13
β-strand	182	1	13
α-helix	198-224	27
α-helix	233-235	3
α-helix	238-263	26
α-helix	284-291	8
α-helix	295-315	21
α-helix	325-327	3
α-helix	328-335	8
α-helix	337-340	4
α-helix	345-347	3
β-strand	352-354	3	14
β-strand	361-363	3	14
α-helix	372-376	5
α-helix	377-407	31
α-helix	414-456	43
α-helix	463-465	3
α-helix	477-489	13
α-helix	493-496	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	A	protein	248	Homo sapiens	Q5JWF2 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	337	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	57	Homo sapiens	P59768 (AlphaFold model)
Nb35	N	protein	126	Homo sapiens
Frizzled-3	R	protein	520	Homo sapiens	Q9NPG1 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8JHI_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas (chains A)
NSKTEDKRAQKRAEKKRSKLIDKQLQDEKMGYMCTHRLLLLGADNSGKSTIVKQMRILHG
GSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRL
QEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATP
EPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCRDIIQRM
HLRQYELL

Sequence of entity 2 (B), FASTA

>8JHI_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
LDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHW
GTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSI
YNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGH
TGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFAT
GSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKAD
RAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>8JHI_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
TASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFR

Sequence of entity 4 (N), FASTA

>8JHI_4 Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTV

Sequence of entity 5 (R), FASTA

>8JHI_5 Frizzled-3 (chains R)
HSLFSCEPITLRMCQDLPYNTTFMPNLLNHYDQQTAALAMEPFHPMVNLDCSRDFRPFLC
ALYAPICMEYGRVTLPCRRLCQRAYSECSKLMEMFGVPWPEDMECSRFPDCDEPYPRLVD
LNLAGEPTEGAPVAVQRDYGFWCPRELKIDPDLGYSFLHVRDCSPPCPNMYFRREELSFA
RYFIGLISIICLSATLFTFLTFLIDVTRFRYPERPIIFYAVCYMMVSLIFFIGFLLEDRV
ACNASIPAQYKASTVTQGSHNKACTMLFMILYFFTMAGSVWWVILTITWFLAAVPKWGSE
AIEKKALLFHASAWGIPGTLTIILLAMNKIEGDNISGVCFVGLYDVDALRYFVLAPLCLY
VVVGVSLLLAGIISLNRVRIEIPLEKENQDKLVKFMIRIGVFSILYLVPLLVVIGCYFYE
QAYRGIWETTWIQERCREYHIPCPYQVTQMSRPDLILFLMKYLMALIVGIPSVFWVGSKK
TCFEWASFFHGRRKKEIVNESRQVLQEPDFAQSLLRDPNT

Primary citation

A framework for Frizzled-G protein coupling and implications to the PCP signaling pathways. Zhang, Z., Lin, X., Wei, L. et al. Cell Discov (2024) 10:3-3. DOI 10.1038/s41421-023-00627-y · PubMed

Other PDB entries of the same protein (UniProt Q5JWF2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9BKK 2.51 Å, Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
8WW2 2.79 Å, GPR3/Gs complex
8I2G 2.8 Å, FSHR-Follicle stimulating hormone-compound 716340-Gs complex
8JLQ 2.84 Å, Fenoldopam-bound hTAAR1-Gs protein complex
9DQJ 2.9 Å, CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-3945
9YGZ 3.04 Å, Cryo-EM structure of active mutant human green cone opsin (E129Q) in complex with…
9WNF 3.13 Å, 5HT2AR-miniGq heterotrimer in complex with a selective agonist IHCH-2330
8ZH8 3.19 Å, Human GPR103 -Gq complex bound to QRFP26
9WND 3.2 Å, 5HT2AR-miniGq heterotrimer in complex with a psychedelic psilocin
8JLP 3.23 Å, Ralmitaront(RO-6889450)-bound hTAAR1-Gs protein complex
8GY7 3.3 Å, Cryo-EM structure of ACTH-bound melanocortin-2 receptor in complex with MRAP1 and Gs…
8JHB 3.3 Å, FZD6 Gs complex

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