Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 1), obtained after local refinement. Determined by electron microscopy at 3.5 Å resolution. Released 7 Feb 2024.
Explore 8PB1 in 3D Show helices and sheets RCSB PDB PDBe
8PB1 contains 17 α-helices and 66 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 33-54 | 22 | |
| α-helix | 56-60 | 5 | |
| α-helix | 77-80 | 4 | |
| α-helix | 115-142 | 28 | |
| α-helix | 153-166 | 14 | |
| α-helix | 186-213 | 28 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 24-27 | 4 | 1 |
| β-strand | 30-36 | 7 | 1 |
| β-strand | 44-49 | 6 | 2 |
| α-helix | 57-58 | 2 | |
| β-strand | 59-62 | 4 | 1 |
| β-strand | 70-71 | 2 | 1 |
| β-strand | 74-79 | 6 | 2 |
| α-helix | 82-84 | 3 | |
| β-strand | 86-92 | 7 | 1 |
| β-strand | 95-108 | 14 | 1 |
| β-strand | 111-112 | 2 | 1 |
| β-strand | 117-118 | 2 | 3 |
| β-strand | 127-129 | 3 | 3 |
| β-strand | 130 | 1 | 4 |
| β-strand | 136-143 | 8 | 3 |
| β-strand | 149 | 1 | 1 |
| β-strand | 153-154 | 2 | 1 |
| β-strand | 165 | 1 | 5 |
| β-strand | 171-179 | 9 | 3 |
| β-strand | 182-192 | 11 | 3 |
| β-strand | 193 | 1 | 5 |
| α-helix | 205 | 1 | |
| β-strand | 206-214 | 9 | 1 |
| β-strand | 217-225 | 9 | 1 |
| β-strand | 231 | 1 | 4 |
| α-helix | 234-236 | 3 | |
| β-strand | 242-243 | 2 | 6 |
| β-strand | 249-252 | 4 | 6 |
| β-strand | 268-274 | 7 | 7 |
| β-strand | 294-296 | 3 | 7 |
| β-strand | 300-303 | 4 | 6 |
| β-strand | 310-316 | 7 | 7 |
| α-helix | 321-323 | 3 | |
| β-strand | 327-329 | 3 | 7 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 47-54 | 8 | 8 |
| β-strand | 55 | 1 | 9 |
| β-strand | 60-68 | 9 | 8 |
| β-strand | 76-81 | 6 | 10 |
| β-strand | 96-101 | 6 | 10 |
| β-strand | 105-109 | 5 | 8 |
| β-strand | 120-128 | 9 | 10 |
| β-strand | 131-133 | 3 | 10 |
| β-strand | 138-140 | 3 | 10 |
| β-strand | 146 | 1 | 9 |
| α-helix | 150-152 | 3 | |
| α-helix | 155 | 1 | |
| β-strand | 156-160 | 5 | 11 |
| β-strand | 163-168 | 6 | 11 |
| β-strand | 179-183 | 5 | 12 |
| β-strand | 190-193 | 4 | 12 |
| β-strand | 214-219 | 6 | 11 |
| β-strand | 225-230 | 6 | 12 |
| β-strand | 234 | 1 | 13 |
| β-strand | 240 | 1 | 13 |
| α-helix | 243-247 | 5 | |
| β-strand | 251-253 | 3 | 12 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 31-36 | 6 | 14 |
| β-strand | 40-42 | 3 | 15 |
| β-strand | 47-53 | 7 | 14 |
| β-strand | 69-74 | 6 | 16 |
| β-strand | 77-81 | 5 | 16 |
| α-helix | 90-92 | 3 | |
| β-strand | 94-99 | 6 | 14 |
| α-helix | 102-103 | 2 | |
| β-strand | 107-112 | 6 | 16 |
| β-strand | 125-131 | 7 | 16 |
| β-strand | 132-134 | 3 | 15 |
| α-helix | 136-140 | 5 | |
| β-strand | 141-148 | 8 | 17 |
| β-strand | 153-159 | 7 | 17 |
| β-strand | 168-176 | 9 | 18 |
| β-strand | 180-188 | 9 | 18 |
| β-strand | 196-198 | 3 | 17 |
| β-strand | 211-220 | 10 | 18 |
| β-strand | 223-225 | 3 | 18 |
| β-strand | 230-233 | 4 | 18 |
| β-strand | 238 | 1 | 17 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Interleukin-12 subunit alpha | A | protein | 231 | Mus musculus | P43431 (AlphaFold model) |
| Interleukin-12 subunit beta | B | protein | 313 | Mus musculus | P43432 (AlphaFold model) |
| Interleukin-12 receptor subunit beta-1,Death-associated protein kinase 1 | C | protein | 572 | Mus musculus | Q60837 (AlphaFold model), Q80YE7 (AlphaFold model) |
| Interleukin-12 receptor subunit beta-2,Calmodulin-1 | D | protein | 769 | Mus musculus | P0DP26, P97378 |
>8PB1_1 Interleukin-12 subunit alpha (chains A) RVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCL PLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQ NHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRV VTINRVMGYLSSAGTSDEVDGGSGGSGLNDIFEAQKIEWHEGRTKHHHHHH
>8PB1_2 Interleukin-12 subunit beta (chains B) MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEF LDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWL VQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEE TLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTP HSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSS CSKWACVPCRVRS
>8PB1_3 Interleukin-12 receptor subunit beta-1,Death-associated protein kinase 1 (chains C) QLGASGPGDGCCVEKTSFPEGASGSPLGPRNLSCYRVSKTDYECSWQYDGPEDNVSHVLW CCFVPPNHTHTGQERCRYFSSGPDRTVQFWEQDGIPVLSKVNFWVESRLGNRTMKSQKIS QYLYNWTKTTPPLGHIKVSQSHRQLRMDWNVSEEAGAEVQFRRRMPTTNWTLGDCGPQVN SGSGVLGDIRGSMSESCLCPSENMAQEIQIRRRRRLSSGAPGGPWSDWSMPVCVPPEVLP QAKIKFLVEPLNQGGRRRLTMQGQSPQLAVPEGCRGRPGAQVKKHLVLVRMLSCRCQAQT SKTVPLGKKLNLSGATYDLNVLAKTRFGRSTIQKWHLPAQELTETRALNVSVGGNMTSMQ WAAQAPGTTYCLEWQPWFQHRNHTHCTLIVPEEEDPAKMVTHSWSSKPTLEQEECYRITV FASKNPKNPMLWATVLSSYYFGGNASRAGTPRHVSVRNQTGDSVSVEWTASQLSTCPGVL TQYVVRCEAEDGAWESEWLVPPTKTQVTLDGLRSRVMYKVQVRADTARLPGAWSHPQRFS FEGTGGSGGSGGAARKKWKQSVRLISLCQRLS
>8PB1_4 Interleukin-12 receptor subunit beta-2,Calmodulin-1 (chains D) NIDVCKLGTVTVQPAPVIPLGSAANISCSLNPKQGCSHYPSSNELILLKFVNDVLVENLH GKKVHDHTGHSSTFQVTNLSLGMTLFVCKLNCSNSQKKPPVPVCGVEISVGVAPEPPQNI SCVQEGENGTVACSWNSGKVTYLKTNYTLQLSGPNNLTCQKQCFSDNRQNCNRLDLGINL SPDLAESRFIVRVTAINDLGNSSSLPHTFTFLDIVIPLPPWDIRINFLNASGSRGTLQWE DEGQVVLNQLRYQPLNSTSWNMVNATNAKGKYDLRDLRPFTEYEFQISSKLHLSGGSWSN WSESLRTRTPEEEPVGILDIWYMKQDIDYDRQQISLFWKSLNPSEARGKILHYQVTLQEV TKKTTLQNTTRHTSWTRVIPRTGAWTASVSAANSKGASAPTHINIVDLCGTGLLAPHQVS AKSENMDNILVTWQPPKKADSAVREYIVEWRALQPGSITKFPPHWLRIPPDNMSALISEN IKPYICYEIRVHALSESQGGCSSIRGDSKHKAPVSGPHITAITEKKERLFISWTHIPFPE QRGCILHYRIYWKERDSTAQPELCEIQYRRSQNSHPISSLQPRVTYVLWMTAVTAAGESP QGNEREFCPQGKANGTGGSGGSGGLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSL GQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGY ISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
| ID | Name | Formula | Copies |
|---|---|---|---|
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 4 |
Structures of complete extracellular receptor assemblies mediated by IL-12 and IL-23. Bloch, Y., Felix, J., Merceron, R. et al. Nat Struct Mol Biol (2024) 31:591-597. DOI 10.1038/s41594-023-01190-6 · PubMed
Other PDB entries of the same protein (UniProt P43431 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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