8SOW: F10 single chain fragment variable

Name: PDB 8SOW: Structure of the complex formed by human interleukin-2 and scFv F10
Published: 2024-09-04
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: cytokine

Structure of the complex formed by human interleukin-2 and scFv F10. Determined by X-ray diffraction at 1.71 Å resolution. Released 4 Sept 2024.

Method: X-ray diffraction
Resolution: 1.71 Å
Organism: Homo sapiens
Chains: 2
Atoms: 2,834
Mol. weight: 41.27 kDa
Released: 4 Sept 2024

Explore 8SOW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8SOW contains 13 α-helices and 27 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	7-29	23
α-helix	36-39	4
β-strand	44	1	6
β-strand	47	1	7
α-helix	53-56	4
α-helix	57-60	4
α-helix	63-70	8
α-helix	83-97	15
β-strand	107	1	7
β-strand	112	1	6
α-helix	114-129	16

Chain C: 6 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	1
β-strand	11-12	2	2
β-strand	18-25	8	1
β-strand	34-39	6	3
β-strand	46-51	6	3
β-strand	57-59	3	3
β-strand	67-72	6	1
β-strand	77-82	6	1
α-helix	87-89	3
β-strand	91-97	7	3
β-strand	103-104	2	3
β-strand	108-110	3	3
β-strand	111-112	2	2
β-strand	138-141	4	4
β-strand	144-146	3	5
β-strand	153-159	7	4
β-strand	167-172	6	5
α-helix	177-178	2
β-strand	179-183	5	5
β-strand	187-188	2	5
α-helix	189	1
β-strand	196-200	5	4
β-strand	204-209	6	4
α-helix	214-216	3
β-strand	218-224	7	5
α-helix	231	1
β-strand	232	1	5
α-helix	233	1
β-strand	236-239	4	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
F10 single chain fragment variable	C	protein	241	Homo sapiens
Interleukin-2	A	protein	139	Homo sapiens	P60568 (AlphaFold model)

Sequence of entity 1 (C), FASTA

>8SOW_1 F10 single chain fragment variable (chains C)
EVQLQESGPGLVAPSQSLSITCTVSGFSLTNYDISWIRQPPGKGLEWLGVIWTGGGTNYN
SGFMSRLSITKDNSKSQVFLKMNSLQTDDTAIYYCVRQGRSPYWGQGTLVTVSAGILGSG
GGGSGGGGSGGGGSDIQVTQSPSSLSVSLGDRVTITCKASKDIYNRLAWYQQKPGNAPRL
LISGATSLETGVPSRFSGSGSGKDYTLTITSLQTEDVATYYCQQSWDTPYTFGGGTKLEI
K

Sequence of entity 2 (A), FASTA

>8SOW_2 Interleukin-2 (chains A)
AGSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQ
CLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEF
LNRWITFCQSIISTLTAAA

Primary citation

Engineered cytokine/antibody fusion proteins improve IL-2 delivery to pro-inflammatory cells and promote antitumor activity. Leonard, E.K., Tomala, J., Gould, J.R. et al. JCI Insight (2024) 9. DOI 10.1172/jci.insight.173469 · PubMed

Other PDB entries of the same protein (UniProt P60568 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8SOZ 1.64 Å, Structure of the complex formed by human interleukin-2 and scFv 602
7M2G 1.79 Å, INTERLEUKIN-2 (human) mutant P65K, C125S
4NEJ 1.92 Å, Small molecular fragment bound to crystal contact interface of Interleukin-2
4NEM 1.93 Å, Small molecular fragment bound to crystal contact interface of Interleukin-2
1M48 1.95 Å, Crystal Structure of Human IL-2 Complexed with (R)-N-[2-[1-(Aminoiminomethyl)-3-piperidin…
5LQB 1.95 Å, Complex structure of human IL2 mutant, Proleukin, with Fab fragment of NARA1 antibody
1M47 1.99 Å, Crystal Structure of Human Interleukin-2
1M49 2.0 Å, Crystal Structure of Human Interleukin-2 Complexed with SP-1985
1M4B 2.15 Å, Crystal Structure of Human Interleukin-2 K43C Covalently Modified at C43 with…
1M4A 2.18 Å, Crystal Structure of Human Interleukin-2 Y31C Covalently Modified at C31 with…
1NBP 2.2 Å, Crystal Structure Of Human Interleukin-2 Y31C Covalently Modified At C31 With…
7RA9 2.2 Å, Designed StabIL-2 seq1

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