8T3O: TUG-891 bound FFA4-Gq complex

Cryo-EM structure of the TUG-891 bound FFA4-Gq complex. Determined by electron microscopy at 3.06 Å resolution. Released 17 Jan 2024.

Method: Electron microscopy
Resolution: 3.06 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 9,109
Mol. weight: 134.19 kDa
Ligands: PLM, 2Y5, YN9
Released: 17 Jan 2024

Explore 8T3O in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8T3O contains 36 α-helices and 67 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-35	2	9
β-strand	38-39	2	10
β-strand	72-76	5	9
β-strand	79-83	5	9
β-strand	85	1	10
α-helix	99-101	3
β-strand	105-106	2	11
β-strand	107-108	2	10
β-strand	109-111	3	12
α-helix	118-129	12
α-helix	132-134	3
α-helix	137	1
β-strand	138-139	2	11
β-strand	142-144	3	12
α-helix	146-155	10
α-helix	160-163	4
α-helix	165-169	5
α-helix	172-173	2
α-helix	184-202	19
β-strand	215	1	12
α-helix	223-242	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	45-51	7	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
α-helix	95	1
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	176-181	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	220-223	4	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	303-307	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-341	6	2

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-43	14
α-helix	53-55	3

Chain N: 5 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	13
β-strand	11-12	2	14
β-strand	18-25	8	13
α-helix	29-31	3
β-strand	33-39	7	15
β-strand	45-51	7	15
β-strand	58-60	3	15
β-strand	68-73	6	13
β-strand	78-83	6	13
α-helix	88-90	3
β-strand	92-94	3	16
β-strand	95-99	5	15
β-strand	110-111	2	15
β-strand	115-117	3	16
β-strand	118-119	2	14
α-helix	138-140	3
β-strand	141-143	3	17
β-strand	147-149	3	18
β-strand	156-162	7	17
β-strand	167	1	19
β-strand	173	1	19
β-strand	175-180	6	18
β-strand	187-191	5	18
β-strand	195-196	2	18
α-helix	197	1
β-strand	204-208	5	17
β-strand	212-217	6	17
β-strand	226-232	7	18
α-helix	238	1
β-strand	240	1	18
β-strand	244-247	4	18

Chain R: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	36-70	35
α-helix	73-75	3
α-helix	78-88	11
α-helix	92-101	10
α-helix	108-138	31
α-helix	139-143	5
α-helix	152-175	24
β-strand	177-181	5	1
β-strand	192-196	5	1
α-helix	201-213	13
α-helix	217-243	27
α-helix	249-255	7
α-helix	258-276	19
α-helix	279-290	12
α-helix	300-320	21

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Free fatty acid receptor 4	R	protein	297	Homo sapiens	Q5NUL3 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	342	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	57	Homo sapiens	P59768 (AlphaFold model)
Guanine nucleotide-binding protein G(q)	A	protein	230	Homo sapiens
scFv16	N	protein	266	Mus musculus

Sequence of entity 1 (R), FASTA

>8T3O_1 Free fatty acid receptor 4 (chains R)
RTRFPFFSDVKGDHRLVLAAVETTVLVLIFAVSLLGNVCALVLVARRRRRGATACLVLNL
FCADLLFISAIPLVLAVRWTEAWLLGPVACHLLFYVMTLSGSVTILTLAAVSLERMVCIV
HLQRGPGRRARAVLLALIWGYSAVAALPLCVFFRVVPQRISICTLIWPTIPGEISWDVSF
VTLNFLVPGLVIVISYSKILQITKASRKRLTVSLAYSESHQIRVSQQDFRLFRTLFLLMV
SFFIMWSPIIITILLILIQNFKQDLVIWPSLFFWVVAFTFANSALNPILYNMTLCRN

Sequence of entity 2 (B), FASTA

>8T3O_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAM
HWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNIC
SIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFT
GHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAF
ATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALK
ADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSS

Sequence of entity 3 (G), FASTA

>8T3O_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
TASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFR

Sequence of entity 4 (A), FASTA

>8T3O_4 Guanine nucleotide-binding protein G(q) (chains A)
VSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMTSGIFETKFQV
DKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRT
ISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRKE
FVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNLREYNLV

Sequence of entity 5 (N), FASTA

>8T3O_5 scFv16 (chains N)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELLEENLYFQGASHHHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
PLM	Palmitic acid	C16 H32 O2	1
2Y5	(2R)-1-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)…	C47 H84 O16 P2	1
YN9	3-{4-[(4-fluoro-4'-methyl[1,1'-biphenyl]-2-yl)methoxy]phenyl}propanoic acid	C23 H21 F O3	1

Primary citation

Structural basis for the ligand recognition and signaling of free fatty acid receptors. Zhang, X., Guseinov, A.A., Jenkins, L. et al. Sci Adv (2024) 10:eadj2384-eadj2384. DOI 10.1126/sciadv.adj2384 · PubMed

Other PDB entries of the same protein (UniProt Q5NUL3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8G59 2.64 Å, Cryo-EM structure of the TUG891 bound GPR120-Giq complex
24XB 2.65 Å, Cryo-EM Structure of the Apo FFAR4-Gq Complex
24QH 2.74 Å, Cryo-EM Structure of the FFAR4-Gi complex with unknown density
8ID6 2.8 Å, Cryo-EM structure of the oleic acid bound GPR120-Gi complex
8IYS 2.95 Å, TUG891-bound FFAR4 in complex with Gq
9XFI 2.99 Å, Cryo-EM structure of the Bavachalcone bound FFAR4-Giq complex
8ID8 3.0 Å, Cryo-EM structure of the TUG891 bound GPR120-Gi complex
8ID9 3.0 Å, Cryo-EM structure of the eicosapentaenoic acid bound GPR120-Gi complex
8H4I 3.06 Å, DHA-bound FFAR4 in complex with Gs
8H4L 3.07 Å, DHA-bound FFAR4 in complex with Gq
8H4K 3.1 Å, GW9508-bound FFAR4 in complex with Gq
8ID3 3.1 Å, Cryo-EM structure of the 9-hydroxystearic acid bound GPR120-Gi complex

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