8IYS: TUG891-bound FFAR4

TUG891-bound FFAR4 in complex with Gq. Determined by electron microscopy at 2.95 Å resolution. Released 21 Jun 2023.

Method: Electron microscopy
Resolution: 2.95 Å
Organism: Homo sapiens
Chains: 5
Atoms: 9,671
Mol. weight: 154.92 kDa
Ligands: YN9, GDP
Released: 21 Jun 2023

Explore 8IYS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8IYS contains 41 α-helices and 62 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 18 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-29	23
β-strand	33-40	8	1
α-helix	47-57	11
α-helix	63-90	28
α-helix	98-108	11
α-helix	122-130	9
α-helix	133-140	8
α-helix	142-144	3
α-helix	151-155	5
α-helix	158-161	4
α-helix	170-174	5
β-strand	184-190	7	1
β-strand	193-199	7	1
α-helix	207-209	3
α-helix	212-214	3
β-strand	219-225	7	1
β-strand	232	1	2
β-strand	240	1	2
α-helix	241-253	13
α-helix	256-258	3
β-strand	262-268	7	1
α-helix	270-277	8
α-helix	282-284	3
α-helix	297-308	12
β-strand	318-322	5	1
α-helix	331-353	23

Chain B: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	10-24	15
α-helix	30-33	4
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	175-181	7	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	208-212	5	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	306-307	2	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain E: 4 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	11-12	2	11
β-strand	15-25	11	10
α-helix	29-31	3
β-strand	33-39	7	12
β-strand	45-51	7	12
β-strand	58-60	3	12
β-strand	68-73	6	10
β-strand	78-86	9	10
α-helix	88-90	3
β-strand	92-99	8	12
β-strand	111	1	12
β-strand	115-117	3	12
β-strand	118-119	2	11
β-strand	141-143	3	13
β-strand	147-148	2	14
β-strand	156-162	7	13
β-strand	167	1	15
β-strand	173	1	15
β-strand	175-180	6	14
β-strand	186-191	6	14
β-strand	195-196	2	14
α-helix	197	1
β-strand	204-209	6	13
β-strand	212-217	6	13
β-strand	226-232	7	14
α-helix	238	1
β-strand	244-246	3	14

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	30-43	14
α-helix	53-55	3
α-helix	56-58	3

Chain R: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	24-26	3
α-helix	36-66	31
α-helix	73-89	17
α-helix	92-101	10
α-helix	108-145	38
α-helix	152-168	17
α-helix	172-175	4
β-strand	177-181	5	16
β-strand	192-196	5	16
α-helix	201-212	12
α-helix	213-217	5
α-helix	218-242	25
α-helix	252-290	39
α-helix	300-311	12
α-helix	313-321	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(q) subunit alpha	A	protein	357	Homo sapiens	P50148 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Homo sapiens	P62873 (AlphaFold model)
scFv16	E	protein	247	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Free fatty acid receptor 4	R	protein	361	Homo sapiens	Q5NUL3 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8IYS_1 Guanine nucleotide-binding protein G(q) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKARRELKLLLLGTGESGKSTFIKQMRIIHGSG
YSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFEN
PYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPT
TGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCATDTENIRAAAAFVFAAVKDTILQLNLKEYNLV

Sequence of entity 2 (B), FASTA

>8IYS_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (E), FASTA

>8IYS_3 scFv16 (chains E)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL

Sequence of entity 4 (G), FASTA

>8IYS_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (R), FASTA

>8IYS_5 Free fatty acid receptor 4 (chains R)
MSPECARAAGDAPLRSLEQANRTRFPFFSDVKGDHRLVLAAVETTVLVLIFAVSLLGNVC
ALVLVARRRRRGATACLVLNLFCADLLFISAIPLVLAVRWTEAWLLGPVACHLLFYVMTL
SGSVTILTLAAVSLERMVCIVHLQRGVRGPGRRARAVLLALIWGYSAVAALPLCVFFRVV
PQRLPGADQEISICTLIWPTIPGEISWDVSFVTLNFLVPGLVIVISYSKILQITKASRKR
LTVSLAYSESHQIRVSQQDFRLFRTLFLLMVSFFIMWSPIIITILLILIQNFKQDLVIWP
SLFFWVVAFTFANSALNPILYNMTLCRNEWKKIFCCFWFPEKGAILTDTSVKRNDLSIIS
G

Ligands and cofactors

ID	Name	Formula	Copies
YN9	3-{4-[(4-fluoro-4'-methyl[1,1'-biphenyl]-2-yl)methoxy]phenyl}propanoic acid	C23 H21 F O3	1
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1

Primary citation

Structural basis of omega-3 fatty acid receptor FFAR4 activation and G protein coupling selectivity. Yin, H., Inoue, A., Ma, Z. et al. Cell Res (2023) 33:644-647. DOI 10.1038/s41422-023-00835-x · PubMed

Other PDB entries of the same protein (UniProt P50148 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9VAW 2.24 Å, Cryo-EM structure of human Neurotensin Receptor 1 (hNTSR1)-Gq (delipidated) complex in…
9M1H 2.55 Å, Cryo-EM structure of PGE2-EP1-Gq complex
9XIX 2.58 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the C-state
8THK 2.6 Å, Cryo-EM structure of A61603-bound alpha-1A-adrenergic receptor in complex with…
9XH2 2.6 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the E-state
8G59 2.64 Å, Cryo-EM structure of the TUG891 bound GPR120-Giq complex
24XB 2.65 Å, Cryo-EM Structure of the Apo FFAR4-Gq Complex
8XGO 2.68 Å, a peptide receptor complex structure
9K27 2.68 Å, PrRP31 bound prolactin-releasing peptide receptor coupled with Gq protein complex
9XJP 2.74 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the E-state, chemokine domain fully…
9LL9 2.76 Å, DOI-bound Serotonin 2A (5-HT2A) receptor-Gq complex
7F6I 2.8 Å, Cryo-EM structure of human bradykinin receptor BK2R in complex Gq proteins and kallidin

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