8TG8: Red beta C-terminal domain

Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 3. Determined by X-ray diffraction at 1.58 Å resolution. Released 13 Mar 2024.

Method: X-ray diffraction
Resolution: 1.58 Å
Organisms: Escherichia phage Lambda, Escherichia coli
Chains: 2
Atoms: 652
Mol. weight: 10.73 kDa
Released: 13 Mar 2024

Explore 8TG8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8TG8 contains 7 α-helices and 2 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	195	1
β-strand	196	1	1
α-helix	197-198	2
α-helix	199-212	14
α-helix	216-220	5
α-helix	221-228	8
α-helix	235-237	3
β-strand	239	1	1
α-helix	240-256	17

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Recombination protein bet	A	protein	84	Escherichia phage Lambda	P03698
Trp-met-asp-phe-asp-asp-asp-ile-pro-phe	B	protein	10	Escherichia coli	P0AGE0 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8TG8_1 Recombination protein bet (chains A)
GSHMTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSE
LTQAEAVKALGFLKQKAAEQKVAA

Sequence of entity 2 (B), FASTA

>8TG8_2 TRP-MET-ASP-PHE-ASP-ASP-ASP-ILE-PRO-PHE (chains B)
WMDFDDDIPF

Primary citation

Structural Basis for the Interaction of Red beta Single-Strand Annealing Protein with Escherichia coli Single-Stranded DNA-Binding Protein. Zakharova, K., Liu, M., Greenwald, J.R. et al. J Mol Biol (2024) 436:168590-168590. DOI 10.1016/j.jmb.2024.168590 · PubMed

Other PDB entries of the same protein (UniProt P03698), best resolution first:

8TFU 1.48 Å, Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 1
8TGC 1.48 Å, Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 4
8TG7 1.77 Å, Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 2
6M9K 2.3 Å, Crystal structure of lambda exonuclease in complex with the Red beta C-terminal domain
7UJL 3.3 Å, Bacteriophage Lambda Red-Beta N-terminal domain helical assembly in complex with dsDNA

Browse structure collections

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