8U02: D2 dopamine receptor

CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant and dopamine. Determined by electron microscopy at 3.28 Å resolution. Released 21 Aug 2024.

Method: Electron microscopy
Resolution: 3.28 Å
Organism: Homo sapiens
Chains: 4
Atoms: 7,774
Mol. weight: 138.22 kDa
Ligands: LDP
Released: 21 Aug 2024

Explore 8U02 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8U02 contains 40 α-helices and 38 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-23	21
α-helix	30-33	4
α-helix	34-36	3
β-strand	47-51	5	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	89-94	6	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	121-125	5	6
β-strand	134-139	6	6
β-strand	146-153	8	7
β-strand	156-161	6	7
β-strand	165-170	6	7
β-strand	175-181	7	7
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	208-212	5	8
β-strand	217-222	6	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	303-308	6	10
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	336-339	4	4

Chain B: 18 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-39	6	2
α-helix	46-53	8
α-helix	64-68	5
α-helix	70-91	22
α-helix	93-95	3
α-helix	99-112	14
α-helix	122-132	11
α-helix	135-142	8
α-helix	151-158	8
α-helix	160-165	6
β-strand	186-192	7	2
β-strand	195-201	7	2
α-helix	209-213	5
α-helix	214-216	3
β-strand	221-227	7	2
α-helix	228-232	5
β-strand	234	1	3
α-helix	236-238	3
β-strand	242	1	3
α-helix	243-255	13
β-strand	265-270	6	2
α-helix	272-278	7
α-helix	297-309	13
β-strand	319-323	5	2
α-helix	332-351	20

Chain C: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-41	4
α-helix	42-60	19
α-helix	68-82	15
α-helix	83-87	5
α-helix	88-96	9
α-helix	104-137	34
α-helix	139-142	4
α-helix	146-167	22
α-helix	168-171	4
β-strand	178	1	1
β-strand	181	1	1
α-helix	187-194	8
α-helix	195-199	5
α-helix	200-223	24
α-helix	362-398	37
α-helix	405-427	23
α-helix	431-440	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
D(2) dopamine receptor	R	protein	443	Homo sapiens	P14416 (AlphaFold model)
Guanine nucleotide-binding protein G(o) subunit alpha	B	protein	354	Homo sapiens	P09471 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	A	protein	358	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (R), FASTA

>8U02_1 D(2) dopamine receptor (chains R)
MDPLNLSWYDDDLERQNWSRPFNGSDGKADRPHYNYYATLLTLLIAVIVFGNVLVCMAVS
REKALQTTTNYLIVSLAVADLLVATLVMPWVVYLEVVGEWKFSRIHCDIFVTLDVMMCTA
SILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISIVWVLSFTISCPLLFGLNNADQN
ECIIANPAFVVYSSIVSFYVPFIVTLLVYIKIYIVLRRRRKRVNTKRSSRAFRAHLRAPL
KGNCTHPEDMKLCTVIMKSNGSFPVNRRRVEAARRAQELEMEMLSSTSPPERTRYSPIPP
SHHQLTLPDPSHHGLHSTPDSPAKPEKNGHAKDHPKIAKIFEIQTMPNGKTRTSLKTMSR
RKLSQQKEKKATQMLAIVLGVFIICWLPFFITHILNIHCDCNIPPVLYSAFTWLGYVNSA
VNPIIYTTFNIEFRKAFLKILHC

Sequence of entity 2 (B), FASTA

>8U02_2 Guanine nucleotide-binding protein G(o) subunit alpha (chains B)
MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGESTIVKQMKIIHEDG
FSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMVCDVVSRMEDTEPF
SAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRV
KTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDET
TNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGPNTYEDA
AAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY

Sequence of entity 3 (A), FASTA

>8U02_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains A)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>8U02_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
LDP	L-dopamine	C8 H11 N O2	1

Primary citation

A neurodevelopmental disorder mutation locks G proteins in the transitory pre-activated state. Knight, K.M., Krumm, B.E., Kapolka, N.J. et al. Nat Commun (2024) 15:6643-6643. DOI 10.1038/s41467-024-50964-z · PubMed

Other PDB entries of the same protein (UniProt P14416 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5AER 2.19 Å, Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with D2 dopamine…
9BS9 2.28 Å, Local refinement of DRD2 bound to LSD in complex with a mini-GoA and scFv16 obtained by…
9BSB 2.32 Å, Global reconstruction of DRD2 bound to LSD in complex with a mini-GoA and scFv16…
7JVR 2.8 Å, Cryo-EM structure of Bromocriptine-bound dopamine receptor 2 in complex with Gi protein
6CM4 2.87 Å, Structure of the D2 Dopamine Receptor Bound to the Atypical Antipsychotic Drug Risperidone
8IRS 3.0 Å, Dopamine Receptor D2R-Gi-Rotigotine complex
6LUQ 3.1 Å, Haloperidol bound D2 dopamine receptor structure inspired discovery of subtype selective…
7DFP 3.1 Å, Human dopamine D2 receptor in complex with spiperone
8TZQ 3.2 Å, CryoEM structure of D2 dopamine receptor in complex with GoA KE Mutant, scFv16, and…
6VMS 3.8 Å, Structure of a D2 dopamine receptor-G-protein complex in a lipid membrane

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