8UHG: Negative elongation factor E

Structure of paused transcription complex Pol II-DSIF-NELF - poised post-translocated. Determined by electron microscopy at 2.7 Å resolution. Released 20 Mar 2024.

Method: Electron microscopy
Resolution: 2.7 Å
Organisms: Homo sapiens, Sus scrofa, synthetic construct
Chains: 20
Atoms: 44,884
Mol. weight: 900.74 kDa
Ligands: MG, ZN
Released: 20 Mar 2024

Explore 8UHG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8UHG contains 281 α-helices and 262 β-strands across 17 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 73 helices, 72 β-strands

Element	Residues	Length	Sheet
β-strand	15-16	2	3
β-strand	20-22	3	4
α-helix	28-34	7
β-strand	43	1	5
β-strand	47	1	6
β-strand	52	1	6
α-helix	53	1
β-strand	56	1	5
β-strand	94-95	2	7
α-helix	97-99	3
α-helix	100-107	8
β-strand	110	1	8
β-strand	117	1	8
α-helix	124-132	9
α-helix	137-149	13
β-strand	190-194	5	9
β-strand	197-201	5	9
β-strand	214-216	3	9
α-helix	218-226	9
α-helix	230-235	6
β-strand	239	1	10
β-strand	243	1	10
α-helix	245-248	4
β-strand	249-250	2	7
α-helix	275-296	22
α-helix	300-318	19
α-helix	326-328	3
β-strand	329	1	11
β-strand	335	1	11
α-helix	336-337	2
α-helix	339-343	5
α-helix	349-352	4
β-strand	358-359	2	12
β-strand	362-369	8	13
β-strand	377-380	4	13
α-helix	382-387	6
β-strand	389-393	5	14
α-helix	399-408	10
β-strand	416-420	5	14
β-strand	426-428	3	14
α-helix	435-438	4
β-strand	445-449	5	14
α-helix	450	1
β-strand	455-459	5	13
α-helix	466-468	3
β-strand	469-476	8	13
β-strand	482-484	3	13
α-helix	489-492	4
β-strand	500-504	5	13
α-helix	509-518	10
β-strand	520	1	13
α-helix	521-524	4
β-strand	526-527	2	15
β-strand	532-533	2	15
α-helix	539-548	10
β-strand	554-555	2	16
α-helix	557-563	7
α-helix	564-566	3
α-helix	573-574	2
β-strand	575	1	17
α-helix	576-577	2
β-strand	579-581	3	18
β-strand	585	1	18
β-strand	586-587	2	16
α-helix	588-595	8
β-strand	602-605	4	19
β-strand	627-631	5	19
β-strand	634-637	4	19
α-helix	642-645	4
α-helix	652-683	32
β-strand	685-686	2	20
α-helix	689-692	4
α-helix	696-721	26
α-helix	727-728	2
α-helix	733-758	26
α-helix	765-771	7
α-helix	778-785	8
β-strand	789-790	2	21
β-strand	793	1	22
β-strand	796	1	22
α-helix	798-799	2
β-strand	802	1	23
β-strand	805	1	23
β-strand	822-823	2	21
α-helix	833-855	23
α-helix	859-868	10
β-strand	872-873	2	24
β-strand	879-880	2	24
β-strand	886-888	3	24
α-helix	891-893	3
β-strand	896	1	25
β-strand	902-905	4	26
α-helix	913-920	8
α-helix	927-933	7
β-strand	934	1	27
α-helix	936-944	9
α-helix	946-969	24
β-strand	976-979	4	26
α-helix	983-993	11
β-strand	1002	1	27
α-helix	1006-1019	14
α-helix	1028-1037	10
α-helix	1039-1048	10
α-helix	1051-1053	3
α-helix	1054-1058	5
α-helix	1062-1079	18
β-strand	1080	1	25
α-helix	1081-1082	2
α-helix	1087-1096	10
α-helix	1097-1100	4
α-helix	1121-1128	8
β-strand	1139-1142	4	28
α-helix	1146-1148	3
α-helix	1151-1161	11
β-strand	1162	1	29
β-strand	1164-1165	2	30
α-helix	1166-1169	4
β-strand	1170-1176	7	31
α-helix	1187-1195	9
β-strand	1211-1216	6	31
α-helix	1218-1223	6
α-helix	1228-1239	12
β-strand	1243-1247	5	31
β-strand	1255-1261	7	31
α-helix	1283-1294	12
β-strand	1297-1299	3	30
β-strand	1301	1	29
β-strand	1306-1310	5	28
β-strand	1319-1322	4	32
β-strand	1328-1331	4	32
β-strand	1335-1339	5	28
α-helix	1343-1348	6
β-strand	1358-1359	2	28
α-helix	1362-1368	7
α-helix	1371-1387	17
α-helix	1395-1405	11
β-strand	1408-1409	2	33
α-helix	1416-1419	4
α-helix	1426-1432	7
α-helix	1435-1445	11
β-strand	1448-1449	2	4
α-helix	1454-1459	6
α-helix	1467-1469	3
β-strand	1471-1475	5	34
α-helix	1477-1481	5

Chain B: 52 helices, 80 β-strands

Element	Residues	Length	Sheet
β-strand	16	1	35
α-helix	19-36	18
α-helix	41-49	9
α-helix	50-54	5
α-helix	55-61	7
α-helix	63-64	2
β-strand	65-67	3	36
β-strand	83-93	11	36
β-strand	97-99	3	37
β-strand	105-107	3	37
α-helix	110-116	7
β-strand	121-133	13	36
β-strand	139-153	15	36
α-helix	168-173	6
β-strand	185-187	3	38
β-strand	190-194	5	38
α-helix	195	1
β-strand	196-200	5	39
β-strand	206-208	3	40
β-strand	215-223	9	40
β-strand	233-240	8	40
α-helix	249-252	4
β-strand	256-259	4	40
β-strand	263	1	41
β-strand	267-268	2	40
α-helix	269-276	8
α-helix	281-288	8
α-helix	295-307	13
α-helix	314-323	10
β-strand	325	1	41
α-helix	332-345	14
α-helix	355-357	3
α-helix	358-376	19
α-helix	381-384	4
α-helix	388-390	3
β-strand	391-394	4	39
α-helix	396-424	29
α-helix	431-434	4
α-helix	438-450	13
β-strand	452-453	2	42
α-helix	459-461	3
β-strand	463-464	2	42
β-strand	467-469	3	38
α-helix	475-482	8
β-strand	484-486	3	39
α-helix	492-495	4
α-helix	503-505	3
β-strand	509	1	43
β-strand	523-525	3	39
β-strand	526	1	43
β-strand	531-532	2	44
α-helix	539-547	9
α-helix	550	1
β-strand	551-552	2	45
α-helix	553-555	3
α-helix	558-560	3
β-strand	565-569	5	45
β-strand	572-577	6	45
α-helix	580-592	13
β-strand	602-606	5	45
β-strand	611-615	5	45
β-strand	621-627	7	44
β-strand	628-629	2	46
β-strand	632-633	2	46
β-strand	636	1	35
α-helix	637-644	8
α-helix	653-658	6
β-strand	662-666	5	44
α-helix	669-672	4
β-strand	675-676	2	44
β-strand	695-696	2	44
α-helix	700-703	4
α-helix	714-716	3
α-helix	719-731	13
β-strand	732	1	47
β-strand	747-751	5	48
β-strand	755	1	49
β-strand	759-760	2	50
α-helix	762-765	4
β-strand	776-782	7	51
β-strand	783-784	2	20
β-strand	793-797	5	51
α-helix	798-802	5
β-strand	808-817	10	48
β-strand	820-821	2	52
β-strand	825-826	2	52
β-strand	827-829	3	53
α-helix	831-833	3
β-strand	837	1	53
β-strand	848	1	54
β-strand	853	1	55
β-strand	854	1	54
α-helix	855	1
β-strand	859-860	2	48
β-strand	865-867	3	55
β-strand	869-872	4	53
α-helix	873	1
β-strand	889-891	3	53
β-strand	894-895	2	55
β-strand	902-911	10	48
β-strand	917-927	11	48
β-strand	934-936	3	51
β-strand	943-949	7	51
β-strand	956	1	56
α-helix	961-963	3
β-strand	965-967	3	51
α-helix	969-971	3
α-helix	978-993	16
β-strand	997-998	2	50
α-helix	1008-1016	9
β-strand	1025-1026	2	51
β-strand	1029	1	56
α-helix	1035	1
β-strand	1036	1	56
α-helix	1037	1
α-helix	1040	1
β-strand	1041-1050	10	51
β-strand	1051	1	47
β-strand	1060-1062	3	13
β-strand	1067	1	57
β-strand	1074	1	57
β-strand	1084-1085	2	12
α-helix	1088-1097	10
α-helix	1100-1104	5
α-helix	1105-1109	5
β-strand	1113-1119	7	3
β-strand	1125	1	3
β-strand	1127	1	58
β-strand	1136	1	58
β-strand	1146-1152	7	3
α-helix	1153-1164	12
β-strand	1170-1172	3	4

Chain C: 10 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	8-14	7	59
β-strand	18-24	7	59
α-helix	28-40	13
β-strand	44-55	12	60
α-helix	61-69	9
β-strand	72	1	60
β-strand	73	1	61
β-strand	99-106	8	60
α-helix	112	1
β-strand	113-116	4	62
α-helix	117-119	3
β-strand	121-122	2	60
β-strand	129	1	61
α-helix	147-148	2
β-strand	149-153	5	62
β-strand	158-168	11	60
α-helix	174-176	3
β-strand	179-185	7	59
α-helix	200-202	3
α-helix	204-206	3
α-helix	218-220	3
β-strand	229-235	7	59
α-helix	241-267	27

Chain D: 10 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	17-19	3
α-helix	24-26	3
β-strand	31	1	34
α-helix	34-49	16
α-helix	55-58	4
α-helix	59-70	12
α-helix	77-89	13
α-helix	94-103	10
α-helix	108-114	7
α-helix	116-118	3
α-helix	124-137	14

Chain E: 12 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	3-23	21
β-strand	26-27	2	63
α-helix	30-33	4
α-helix	37-43	7
α-helix	55-58	4
β-strand	60-64	5	63
β-strand	71-75	5	63
β-strand	81-82	2	64
α-helix	84-97	14
β-strand	101-106	6	63
β-strand	109-110	2	64
α-helix	112-120	9
β-strand	126-131	6	63
α-helix	134-136	3
α-helix	139-141	3
β-strand	147-151	5	33
α-helix	153-163	11
α-helix	167-169	3
β-strand	172-173	2	33
α-helix	178-183	6
α-helix	185-186	2
β-strand	190-197	8	33
β-strand	201-209	9	33

Chain F: 4 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	56	1	65
α-helix	57-58	2
α-helix	59-74	16
α-helix	89-99	11
β-strand	104-109	6	34
β-strand	115-119	5	34
β-strand	124	1	65
α-helix	125	1

Chain G: 4 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	2-13	12	34
α-helix	15-17	3
α-helix	22-34	13
β-strand	38-39	2	34
β-strand	43-54	12	34
α-helix	55-56	2
β-strand	58-59	2	34
α-helix	60	1
β-strand	66-77	12	34
β-strand	84-91	8	66
β-strand	96-101	6	66
β-strand	104-109	6	66
β-strand	117-119	3	67
β-strand	128-130	3	67
β-strand	136-137	2	67
β-strand	142-146	5	66
β-strand	149-153	5	66
β-strand	156-161	6	66
β-strand	169-170	2	66

Chain H: 2 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	4-14	11	18
β-strand	25-32	8	18
β-strand	38-44	7	18
β-strand	55-61	7	18
β-strand	74	1	17
α-helix	84-87	4
β-strand	90-101	12	18
β-strand	111-118	8	18
β-strand	121-128	8	18
α-helix	129-132	4
β-strand	139-147	9	18

9 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Negative elongation factor E	X	protein	380	Homo sapiens	P18615 (AlphaFold model)
Negative elongation factor B	V	protein	554	Homo sapiens	Q8WX92 (AlphaFold model)
Negative elongation factor C/D	W	protein	590	Homo sapiens	Q8IXH7 (AlphaFold model)
DNA-directed RNA polymerase subunit	A	protein	1970	Sus scrofa	A0A8D1DPV6
DNA-directed RNA polymerase subunit beta	B	protein	1174	Sus scrofa	I3LGP4
DNA-directed RNA polymerase II subunit RPB3	C	protein	271	Sus scrofa	I3LCH3
RNA polymerase Rpb4/RPC9 core domain-containing protein	D	protein	142	Sus scrofa	A0A4X1VM56
DNA-directed RNA polymerase II subunit E	E	protein	210	Sus scrofa	I3LSI7
DNA-directed RNA polymerases I, II, and III subunit RPABC2	F	protein	127	Sus scrofa	A0A4X1VEK9
DNA-directed RNA polymerase subunit	G	protein	171	Sus scrofa	A0A4X1VKG7
DNA-directed RNA polymerases I, II, and III subunit RPABC3	H	protein	150	Sus scrofa	I3LCB2
DNA-directed RNA polymerase II subunit RPB9	I	protein	125	Sus scrofa	P60899

8 more molecules are not listed.

Sequence of entity 1 (X), FASTA

>8UHG_1 Negative elongation factor E (chains X)
MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTAT
ATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDL
QESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSA
SPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDR
DREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFV
TYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRD
KRTQIVYSDDVYKENLVDGF

Sequence of entity 2 (V), FASTA

>8UHG_2 Negative elongation factor B (chains V)
MFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLE
FHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHL
PKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTE
LSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAE
LLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGD
LSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVF
KEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRM
ACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALED
FCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKEL
YSQLGEKLEQLDHR

Sequence of entity 3 (W), FASTA

>8UHG_3 Negative elongation factor C/D (chains W)
MAGAVPGAIMDEDYYGSAAEWGDEADGGQQEDDSGEGEDDAEVQQECLHKFSTRDYIMEP
SIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLK
SLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLIS
DAGYQGEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEFAKMVCHGEHTYLF
AQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAML
SKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQDHKHKYIHIL
AYAASVVETWKKNKRVSINKDELKSTSKAVETVHNLCCNENKGASELVAELSTLYQCIRF
PVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETE
HSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDV
IAPPYTSDFVQLFLPILENDSIAGTIKTEGEHDPVTEFIAHCKSNFIMVN

Sequence of entity 4 (A), FASTA

>8UHG_4 DNA-directed RNA polymerase subunit (chains A)
MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDP
RQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVD
SNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKG
HGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEP
RYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAA
HVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVD
FSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYII
RDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWS
TFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQD
TLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHI
NCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEM
GHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKA
HNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKIN
ISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHA
MGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGES
VEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMR
EDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKL
VIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAI
AHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLT
VFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMP
DFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIR
IMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKI
IITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALER
ELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLM
EAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTG
MFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPG
YSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSP
SYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSP
TSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS
YSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPT
SPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPTSPKY
SPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTS
PKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN

Sequence of entity 5 (B), FASTA

>8UHG_5 DNA-directed RNA polymerase subunit beta (chains B)
MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVE
DAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTY
SAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLD
PGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSML
ARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEM
VKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETK
KAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ
KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLS
HLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSP
ILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSE
VSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASG
VVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSP
RNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIV
AIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGM
RHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELEGTNRRYTKRDCSTFLRTSE
TGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEG
ITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGY
HLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSR
DGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGC
RNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Sequence of entity 6 (C), FASTA

>8UHG_6 DNA-directed RNA polymerase II subunit RPB3 (chains C)
MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLH
DEFIAHRLGLIPLTSDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDL
ISNSPRVIPVTSRNRDNDPSDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTA
GVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLR
PETIVLSALSGLKKKLSDLQTQLSHEIQSDV

Sequence of entity 7 (D), FASTA

>8UHG_7 RNA polymerase Rpb4/RPC9 core domain-containing protein (chains D)
MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEV
FMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEG
RFEDEELQQILDDIQTKRSFQY

Sequence of entity 8 (E), FASTA

>8UHG_8 DNA-directed RNA polymerase II subunit E (chains E)
MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTV
LVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVD
MAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVA
RYFGIKRGQVVKIIRPSETAGRYITYRLVQ

Sequence of entity 9 (F), FASTA

>8UHG_9 DNA-directed RNA polymerases I, II, and III subunit RPABC2 (chains F)
MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKY
ERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGV
DELIISD

Sequence of entity 10 (G), FASTA

>8UHG_10 DNA-directed RNA polymerase subunit (chains G)
MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ
PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD
PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLV

Sequence of entity 11 (H), FASTA

>8UHG_11 DNA-directed RNA polymerases I, II, and III subunit RPABC3 (chains H)
MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI
ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGG
LLMRLQGDANNLHGFEVDSRVYLLMKKLAF

Sequence of entity 12 (I), FASTA

>8UHG_12 DNA-directed RNA polymerase II subunit RPB9 (chains I)
MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITH
EVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCG
HRWTE

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	1
ZN	Zinc ion	Zn	8

Primary citation

Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing. Su, B.G., Vos, S.M. Mol Cell (2024) 84:1243. DOI 10.1016/j.molcel.2024.01.023 · PubMed

Other PDB entries of the same protein (UniProt P18615 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8UI0 2.7 Å, Structure of poised transcription complex Pol II-DSIF-NELF - pre-translocated
8JJ6 2.72 Å, Structure of the NELF-BCE complex
5OOB 2.79 Å, Complex of human nuclear cap-binding complex with M7GTP and nelf-E C-terminal peptide
8UHD 2.8 Å, Structure of paused transcription complex Pol II-DSIF-NELF - post-translocated
6GML 3.2 Å, Structure of paused transcription complex Pol II-DSIF-NELF
9J0O 3.3 Å, Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC1-nuc)
9J0P 3.3 Å, Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC2-nuc)
9J0N 3.4 Å, Paused elongation complex of mammalian RNA polymerase II with nucleosome (PEC2-nuc)
8UHA 3.5 Å, Structure of paused transcription complex Pol II-DSIF-NELF - tilted
8W8E 3.9 Å, human co-transcriptional RNA capping enzyme RNGTT
7YCX 4.18 Å, The structure of INTAC-PEC complex
1X5P Solution structure of RRM domain in Parp14

Browse structure collections

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