8JJ6: NELF-BCE complex

Structure of the NELF-BCE complex. Determined by X-ray diffraction at 2.72 Å resolution. Released 30 Aug 2023.

Method

X-ray diffraction

Resolution

2.72 Å

Organism

Homo sapiens

Chains

6

Atoms

11,833

Mol. weight

172.89 kDa

Released

30 Aug 2023

Explore 8JJ6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8JJ6 contains 107 α-helices and 0 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 41 helices, 0 β-strands

Molecules and chains

>8JJ6_1 Negative elongation factor B (chains A, B)
MFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLE
FHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHL
PKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTE
LSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAE
LLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGD
LSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVF
KEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRM
ACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALED
FCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKEL
YSQLGEKLEQLDHRKPSPAQ

>8JJ6_2 Negative elongation factor complex member C/D (chains C, D)
EGEDDAEVQQECLHKFSTRDYIMEPSIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVN
LLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTW
RDLFYKLAEAHPDCLMLNFTVKLISDA

>8JJ6_3 NELF-E (chains E, F)
MLVIPPGMSEEEEALQKKFMKLKKKKKALMALKKQSSSSTTSQGGVKRSLY

Primary citation

Structural basis of the human negative elongation factor NELF-B/C/E ternary complex. Cao, Y., Qin, Y., Zhang, W. et al. Biochem Biophys Res Commun (2023) 677:155-161. DOI 10.1016/j.bbrc.2023.08.019 · PubMed

Related structures

Other PDB entries of the same protein (UniProt Q8WX92 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 8UHG 2.7 Å, Structure of paused transcription complex Pol II-DSIF-NELF - poised post-translocated
• 8UI0 2.7 Å, Structure of poised transcription complex Pol II-DSIF-NELF - pre-translocated
• 8UHD 2.8 Å, Structure of paused transcription complex Pol II-DSIF-NELF - post-translocated
• 6GML 3.2 Å, Structure of paused transcription complex Pol II-DSIF-NELF
• 9J0O 3.3 Å, Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC1-nuc)
• 9J0P 3.3 Å, Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC2-nuc)
• 9J0N 3.4 Å, Paused elongation complex of mammalian RNA polymerase II with nucleosome (PEC2-nuc)
• 8UHA 3.5 Å, Structure of paused transcription complex Pol II-DSIF-NELF - tilted
• 7PKS 3.6 Å, Structural basis of Integrator-mediated transcription regulation
• 8W8E 3.9 Å, human co-transcriptional RNA capping enzyme RNGTT
• 8RBX 4.1 Å, Structure of Integrator-PP2A bound to a paused RNA polymerase II-DSIF-NELF-nucleosome…
• 7YCX 4.18 Å, The structure of INTAC-PEC complex

Browse structure collections

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