8W0E: Human MCM2-7 single hexamer on dsDNA
Cryo-EM structure of a human MCM2-7 single hexamer on dsDNA. Determined by electron microscopy at 3.4 Å resolution. Released 2 Oct 2024.
- Method
- Electron microscopy
- Resolution
- 3.4 Å
- Organisms
- Homo sapiens, Saccharomyces cerevisiae
- Chains
- 8
- Atoms
- 30,082
- Mol. weight
- 565.1 kDa
- Ligands
- ZN, ADP, ATP, MG
- Released
- 2 Oct 2024
Explore 8W0E in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8W0E contains 172 α-helices and 176 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 2: 31 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 182-187 | 6 | |
| α-helix | 189-205 | 17 | |
| α-helix | 214-225 | 12 | |
| β-strand | 229-233 | 5 | 1 |
| α-helix | 234-240 | 7 | |
| α-helix | 242-250 | 9 | |
| α-helix | 252-270 | 19 | |
| α-helix | 272-276 | 5 | |
| β-strand | 282-286 | 5 | 1 |
| β-strand | 291 | 1 | 2 |
| α-helix | 294-296 | 3 | |
| α-helix | 299-301 | 3 | |
| β-strand | 305-315 | 11 | 2 |
| β-strand | 319-325 | 7 | 2 |
| β-strand | 328 | 1 | 3 |
| β-strand | 335 | 1 | 3 |
| α-helix | 336-338 | 3 | |
| β-strand | 339-340 | 2 | 2 |
| α-helix | 341-342 | 2 | |
| β-strand | 362 | 1 | 3 |
| β-strand | 369-379 | 11 | 2 |
| β-strand | 393-399 | 7 | 2 |
| β-strand | 411-419 | 9 | 2 |
| β-strand | 434 | 1 | 4 |
| β-strand | 438-446 | 9 | 2 |
| α-helix | 460-468 | 9 | |
| α-helix | 473-479 | 7 | |
| α-helix | 489-500 | 12 | |
| β-strand | 505 | 1 | 5 |
| β-strand | 513 | 1 | 5 |
| β-strand | 519-523 | 5 | 6 |
| α-helix | 529-539 | 11 | |
| β-strand | 543-547 | 5 | 6 |
| β-strand | 559-562 | 4 | 7 |
| β-strand | 569-572 | 4 | 7 |
| α-helix | 575-578 | 4 | |
| β-strand | 583-587 | 5 | 6 |
| α-helix | 594-606 | 13 | |
| β-strand | 608-611 | 4 | 8 |
| β-strand | 618-621 | 4 | 8 |
| α-helix | 624 | 1 | |
| β-strand | 625-630 | 6 | 6 |
| α-helix | 633-635 | 3 | |
| α-helix | 643-645 | 3 | |
| α-helix | 651-654 | 4 | |
| β-strand | 659-663 | 5 | 6 |
| α-helix | 669-685 | 17 | |
| α-helix | 713-716 | 4 | |
| α-helix | 717-729 | 13 | |
| β-strand | 734 | 1 | 9 |
| α-helix | 740-756 | 17 | |
| α-helix | 760-762 | 3 | |
| α-helix | 764-781 | 18 | |
| β-strand | 785 | 1 | 9 |
| α-helix | 788-804 | 17 | |
| α-helix | 808-817 | 10 | |
| α-helix | 819-822 | 4 | |
| α-helix | 828-848 | 21 | |
Chain 3: 28 helices, 33 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-23 | 14 | |
| α-helix | 31-41 | 11 | |
| β-strand | 46-50 | 5 | 10 |
| α-helix | 51-57 | 7 | |
| α-helix | 59-67 | 9 | |
| α-helix | 69-87 | 19 | |
| α-helix | 89-94 | 6 | |
| β-strand | 99-103 | 5 | 10 |
| α-helix | 118-120 | 3 | |
| β-strand | 123-134 | 12 | 11 |
| α-helix | 135-137 | 3 | |
| β-strand | 138-147 | 10 | 12 |
| β-strand | 154-158 | 5 | 12 |
| β-strand | 177 | 1 | 13 |
| β-strand | 183 | 1 | 13 |
| β-strand | 185-186 | 2 | 12 |
| β-strand | 192-196 | 5 | 12 |
| β-strand | 197-202 | 6 | 11 |
| α-helix | 213-214 | 2 | |
| β-strand | 216-221 | 6 | 11 |
| β-strand | 234-244 | 11 | 11 |
| β-strand | 251-252 | 2 | 14 |
| β-strand | 255-256 | 2 | 15 |
| β-strand | 257-267 | 11 | 11 |
| α-helix | 270-272 | 3 | |
| α-helix | 278-290 | 13 | |
| α-helix | 295-301 | 7 | |
| α-helix | 311-322 | 12 | |
| β-strand | 327-328 | 2 | 16 |
| β-strand | 334-335 | 2 | 16 |
| β-strand | 341-345 | 5 | 17 |
| α-helix | 351-361 | 11 | |
| β-strand | 365-368 | 4 | 17 |
| α-helix | 375-377 | 3 | |
| β-strand | 381-384 | 4 | 18 |
| β-strand | 391-394 | 4 | 18 |
| α-helix | 399-401 | 3 | |
| β-strand | 404-408 | 5 | 17 |
| α-helix | 411-413 | 3 | |
| α-helix | 416-420 | 5 | |
| α-helix | 423-427 | 5 | |
| β-strand | 430-435 | 6 | 19 |
| β-strand | 438-443 | 6 | 19 |
| β-strand | 446-452 | 7 | 17 |
| α-helix | 465-468 | 4 | |
| α-helix | 473-478 | 6 | |
| β-strand | 481-485 | 5 | 17 |
| α-helix | 491-506 | 16 | |
| β-strand | 516 | 1 | 20 |
| β-strand | 545 | 1 | 21 |
| α-helix | 550-553 | 4 | |
| β-strand | 563 | 1 | 21 |
| α-helix | 565-576 | 12 | |
| β-strand | 581-582 | 2 | 22 |
| α-helix | 584-598 | 15 | |
| α-helix | 600-602 | 3 | |
| β-strand | 607-609 | 3 | 23 |
| α-helix | 615-631 | 17 | |
| β-strand | 636-637 | 2 | 22 |
| α-helix | 639-653 | 15 | |
| β-strand | 654-656 | 3 | 23 |
Chain 4: 28 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 159-171 | 13 | |
| α-helix | 192-203 | 12 | |
| β-strand | 207-211 | 5 | 24 |
| α-helix | 212-218 | 7 | |
| α-helix | 220-228 | 9 | |
| α-helix | 230-247 | 18 | |
| β-strand | 259-263 | 5 | 24 |
| β-strand | 267 | 1 | 25 |
| α-helix | 271-273 | 3 | |
| α-helix | 276-278 | 3 | |
| β-strand | 282-292 | 11 | 25 |
| α-helix | 293-295 | 3 | |
| β-strand | 296-306 | 11 | 25 |
| β-strand | 312-316 | 5 | 25 |
| β-strand | 327 | 1 | 26 |
| β-strand | 334 | 1 | 26 |
| β-strand | 337-356 | 20 | 25 |
| α-helix | 365-367 | 3 | |
| β-strand | 369-375 | 7 | 25 |
| β-strand | 387-397 | 11 | 25 |
| β-strand | 400 | 1 | 27 |
| β-strand | 408-409 | 2 | 27 |
| β-strand | 411 | 1 | 28 |
| β-strand | 413-422 | 10 | 25 |
| α-helix | 432-436 | 5 | |
| α-helix | 443-454 | 12 | |
| α-helix | 458-465 | 8 | |
| α-helix | 474-485 | 12 | |
| β-strand | 506-510 | 5 | 29 |
| α-helix | 517-524 | 8 | |
| β-strand | 531-533 | 3 | 29 |
| α-helix | 535-537 | 3 | |
| β-strand | 538 | 1 | 30 |
| α-helix | 540-544 | 5 | |
| β-strand | 545-550 | 6 | 31 |
| β-strand | 555-560 | 6 | 31 |
| α-helix | 562-565 | 4 | |
| β-strand | 570-573 | 4 | 29 |
| α-helix | 581-592 | 12 | |
| β-strand | 595-599 | 5 | 32 |
| β-strand | 604-608 | 5 | 32 |
| β-strand | 612-617 | 6 | 29 |
| α-helix | 618-619 | 2 | |
| α-helix | 620-622 | 3 | |
| α-helix | 630-634 | 5 | |
| α-helix | 638-643 | 6 | |
| β-strand | 646-650 | 5 | 29 |
| α-helix | 656-671 | 16 | |
| α-helix | 684-697 | 14 | |
| β-strand | 701-702 | 2 | 33 |
| α-helix | 704-720 | 17 | |
| α-helix | 731-747 | 17 | |
| β-strand | 752-753 | 2 | 33 |
| α-helix | 755-767 | 13 | |
| α-helix | 768-770 | 3 | |
Chain 5: 25 helices, 36 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 28-41 | 14 | |
| α-helix | 54-65 | 12 | |
| β-strand | 69-73 | 5 | 34 |
| α-helix | 74-80 | 7 | |
| α-helix | 82-90 | 9 | |
| α-helix | 92-111 | 20 | |
| β-strand | 125-129 | 5 | 34 |
| α-helix | 133-136 | 4 | |
| α-helix | 137-139 | 3 | |
| β-strand | 148-158 | 11 | 35 |
| α-helix | 159-161 | 3 | |
| β-strand | 163-168 | 6 | 15 |
| β-strand | 170-172 | 3 | 36 |
| β-strand | 178 | 1 | 36 |
| β-strand | 182-183 | 2 | 15 |
| β-strand | 189 | 1 | 37 |
| β-strand | 191-192 | 2 | 14 |
| α-helix | 193-194 | 2 | |
| β-strand | 196 | 1 | 38 |
| β-strand | 209 | 1 | 38 |
| β-strand | 212-214 | 3 | 36 |
| α-helix | 216-218 | 3 | |
| β-strand | 220-222 | 3 | 15 |
| β-strand | 226-230 | 5 | 35 |
| α-helix | 231-232 | 2 | |
| α-helix | 241-243 | 3 | |
| β-strand | 244-248 | 5 | 35 |
| β-strand | 262-271 | 10 | 35 |
| β-strand | 273 | 1 | 37 |
| β-strand | 289 | 1 | 2 |
| β-strand | 292-301 | 10 | 35 |
| α-helix | 316-327 | 12 | |
| α-helix | 331-336 | 6 | |
| α-helix | 347-358 | 12 | |
| β-strand | 363-364 | 2 | 39 |
| β-strand | 370-371 | 2 | 39 |
| β-strand | 377-380 | 4 | 40 |
| α-helix | 388-397 | 10 | |
| β-strand | 401-405 | 5 | 40 |
| β-strand | 408 | 1 | 40 |
| β-strand | 409 | 1 | 41 |
| β-strand | 412 | 1 | 41 |
| β-strand | 417-420 | 4 | 42 |
| β-strand | 427-430 | 4 | 42 |
| α-helix | 433-436 | 4 | |
| β-strand | 441-445 | 5 | 40 |
| α-helix | 452-464 | 13 | |
| β-strand | 466-470 | 5 | 43 |
| β-strand | 475-479 | 5 | 43 |
| β-strand | 483-488 | 6 | 40 |
| α-helix | 497-499 | 3 | |
| α-helix | 508-513 | 6 | |
| β-strand | 516-519 | 4 | 40 |
| α-helix | 527-542 | 16 | |
| α-helix | 556-569 | 14 | |
| β-strand | 570 | 1 | 20 |
| β-strand | 573-574 | 2 | 44 |
| α-helix | 576-600 | 25 | |
| α-helix | 610-626 | 17 | |
| β-strand | 631-632 | 2 | 44 |
| α-helix | 634-651 | 18 | |
Chain 6: 30 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-36 | 16 | |
| α-helix | 45-51 | 7 | |
| α-helix | 52-54 | 3 | |
| β-strand | 60-64 | 5 | 45 |
| α-helix | 65-71 | 7 | |
| α-helix | 73-81 | 9 | |
| α-helix | 83-99 | 17 | |
| β-strand | 111-115 | 5 | 45 |
| β-strand | 121 | 1 | 4 |
| α-helix | 123-125 | 3 | |
| α-helix | 128-130 | 3 | |
| β-strand | 131 | 1 | 4 |
| β-strand | 134-144 | 11 | 4 |
| α-helix | 145-147 | 3 | |
| β-strand | 148-157 | 10 | 4 |
| β-strand | 164-169 | 6 | 4 |
| α-helix | 170 | 1 | |
| β-strand | 199-209 | 11 | 4 |
| α-helix | 215-216 | 2 | |
| α-helix | 220-222 | 3 | |
| β-strand | 225-229 | 5 | 4 |
| β-strand | 241-252 | 12 | 4 |
| β-strand | 294-305 | 12 | 4 |
| α-helix | 322-328 | 7 | |
| α-helix | 331-341 | 11 | |
| α-helix | 346-354 | 9 | |
| α-helix | 362-373 | 12 | |
| β-strand | 378-379 | 2 | 46 |
| β-strand | 385-386 | 2 | 46 |
| β-strand | 392-396 | 5 | 47 |
| α-helix | 402-412 | 11 | |
| β-strand | 416-419 | 4 | 47 |
| β-strand | 424 | 1 | 32 |
| α-helix | 426-430 | 5 | |
| β-strand | 431-435 | 5 | 48 |
| β-strand | 442-446 | 5 | 48 |
| α-helix | 448-451 | 4 | |
| β-strand | 455-460 | 6 | 47 |
| α-helix | 462-464 | 3 | |
| α-helix | 467-479 | 13 | |
| β-strand | 481-486 | 6 | 49 |
| β-strand | 489-494 | 6 | 49 |
| β-strand | 497-503 | 7 | 47 |
| α-helix | 504-505 | 2 | |
| α-helix | 506-508 | 3 | |
| α-helix | 516-519 | 4 | |
| α-helix | 524-528 | 5 | |
| β-strand | 532-536 | 5 | 47 |
| α-helix | 542-557 | 16 | |
| α-helix | 569-579 | 11 | |
| β-strand | 585-586 | 2 | 50 |
| α-helix | 588-604 | 17 | |
| β-strand | 612 | 1 | 51 |
| α-helix | 618-634 | 17 | |
| β-strand | 639-640 | 2 | 50 |
| α-helix | 642-655 | 14 | |
| β-strand | 658 | 1 | 51 |
Chain 7: 30 helices, 26 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-18 | 12 | |
| α-helix | 32-41 | 10 | |
| β-strand | 47-51 | 5 | 52 |
| α-helix | 52-58 | 7 | |
| α-helix | 60-68 | 9 | |
| α-helix | 71-84 | 14 | |
| α-helix | 85-87 | 3 | |
| α-helix | 98-109 | 12 | |
| α-helix | 128-130 | 3 | |
| β-strand | 135-139 | 5 | 52 |
| α-helix | 140-141 | 2 | |
| α-helix | 149-151 | 3 | |
| α-helix | 154-156 | 3 | |
| β-strand | 160-170 | 11 | 28 |
| β-strand | 174-184 | 11 | 28 |
| β-strand | 190-194 | 5 | 28 |
| β-strand | 199-200 | 2 | 27 |
| β-strand | 205 | 1 | 53 |
| α-helix | 209-214 | 6 | |
| β-strand | 219 | 1 | 53 |
| β-strand | 220-238 | 19 | 28 |
| β-strand | 253-258 | 6 | 28 |
| α-helix | 259-261 | 3 | |
| β-strand | 270-281 | 12 | 28 |
| β-strand | 294-305 | 12 | 28 |
| α-helix | 307-309 | 3 | |
| α-helix | 324-327 | 4 | |
| α-helix | 332-338 | 7 | |
| α-helix | 348-359 | 12 | |
| β-strand | 364 | 1 | 54 |
| β-strand | 371 | 1 | 54 |
| β-strand | 377-381 | 5 | 55 |
| α-helix | 387-395 | 9 | |
| β-strand | 401-405 | 5 | 55 |
| α-helix | 411-413 | 3 | |
| β-strand | 417-418 | 2 | 56 |
| β-strand | 429-430 | 2 | 56 |
| α-helix | 433-436 | 4 | |
| β-strand | 441-445 | 5 | 55 |
| α-helix | 447-449 | 3 | |
| α-helix | 452-464 | 13 | |
| β-strand | 466-469 | 4 | 57 |
| β-strand | 470 | 1 | 30 |
| β-strand | 476-479 | 4 | 57 |
| β-strand | 483-488 | 6 | 55 |
| α-helix | 509-512 | 4 | |
| β-strand | 517-521 | 5 | 55 |
| α-helix | 527-543 | 17 | |
| α-helix | 546-548 | 3 | |
| α-helix | 552-555 | 4 | |
| α-helix | 556-567 | 12 | |
| β-strand | 572 | 1 | 58 |
| α-helix | 576-594 | 19 | |
| α-helix | 603-620 | 18 | |
| β-strand | 624 | 1 | 58 |
| α-helix | 627-644 | 18 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA replication licensing factor MCM2 | 2 | protein | 904 | Homo sapiens | P49736 (AlphaFold model) |
| DNA replication licensing factor MCM3 | 3 | protein | 810 | Homo sapiens | P25205 (AlphaFold model) |
| DNA replication licensing factor MCM4 | 4 | protein | 866 | Homo sapiens | P33991 (AlphaFold model) |
| DNA replication licensing factor MCM5 | 5 | protein | 734 | Homo sapiens | P33992 (AlphaFold model) |
| DNA replication licensing factor MCM6 | 6 | protein | 821 | Homo sapiens | Q14566 |
| DNA replication licensing factor MCM7 | 7 | protein | 719 | Homo sapiens | P33993 |
| DNA (25-mer) | O | DNA | 25 | Saccharomyces cerevisiae | |
| DNA (25-mer) | S | DNA | 25 | Saccharomyces cerevisiae | |
Sequence of entity 1 (2), FASTA
>8W0E_1 DNA replication licensing factor MCM2 (chains 2)
MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTE
GPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQR
DREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGH
SVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAR
EHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQL
HLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGP
FEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHN
NYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFAS
IAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVS
SRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSI
HEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDIL
CVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVL
KKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARI
HLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLV
AEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMI
LQQF
Sequence of entity 2 (3), FASTA
>8W0E_2 DNA replication licensing factor MCM3 (chains 3)
SNAAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKN
EKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTS
CFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKD
EENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVV
GTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQ
LAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYV
LCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMD
RTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSR
FDLLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQED
QQDTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLR
SQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVL
EKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMP
QVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEE
PFSSVEIQAALSKMQDDNQVMVSEGIIFLI
Sequence of entity 3 (4), FASTA
>8W0E_3 DNA replication licensing factor MCM4 (chains 4)
SNAMSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGV
DLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPD
LGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPL
AKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTF
DMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIP
EMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQES
PEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHID
VIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIK
KGILLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKG
SSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLS
IAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEA
YDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDM
RKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPR
TGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITK
DMFEEALRALADDDFLTVTGKTVRLL
Sequence of entity 4 (5), FASTA
>8W0E_4 DNA replication licensing factor MCM5 (chains 5)
MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELK
RHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEV
LQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLT
NIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGE
MPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQ
VDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGG
SRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMR
DPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNS
RCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLH
VSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDS
DRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGV
EGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGE
IQHRMQRKVLYRLK
Sequence of entity 5 (6), FASTA
>8W0E_5 DNA replication licensing factor MCM6 (chains 6)
MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNT
LVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTR
HKIRELTSSRIGLLTRISGQVVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNIC
RNPVCANRRRFLLDTNKSRFVDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGD
KCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLA
CCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHG
NDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTS
GKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQ
TISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDEC
NEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKH
LRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVE
TPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEY
CRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRL
THYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED
Sequence of entity 6 (7), FASTA
>8W0E_6 DNA replication licensing factor MCM7 (chains 7)
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDP
ELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVR
SPQNQYPAELMRRFELYFQGPSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVA
TYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEH
SDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEA
HRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDVKKALLLLLVG
GVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR
DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNA
RCSILAAANPAYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYV
HQHSRQPPSQFEPLDMKLMRRYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATY
TSARTLLAILRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPADV
IFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
Sequence of entity 7 (O), FASTA
>8W0E_7 DNA (25-MER) (chains O)
AAAAAAAAAAAAAAAAAAAAAAAAA
Sequence of entity 8 (S), FASTA
>8W0E_8 DNA (25-MER) (chains S)
TTTTTTTTTTTTTTTTTTTTTTTTT
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 5 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 4 |
| ATP | Adenosine-5'-triphosphate | C10 H16 N5 O13 P3 | 1 |
| MG | Magnesium ion | Mg | 5 |
Primary citation
Multiple mechanisms for licensing human replication origins. Yang, R., Hunker, O., Wise, M. et al. Nature (2024) 636:488-498. DOI 10.1038/s41586-024-08237-8 · PubMed
Other PDB entries of the same protein (UniProt P49736 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 6YA7 1.67 Å, Cdc7-Dbf4 bound to an Mcm2-S40 derived bivalent substrate
- 7CIZ 1.8 Å, Crystal structure of DNAJC9 HBD helix2 in complex with H3.3-H4 dimer and MCM2 HBD
- 5BNX 2.31 Å, Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.3-H4 dimer
- 5JA4 2.42 Å, Crystal structure of human TONSL and MCM2 HBDs binding to a histone H3-H4 tetramer
- 7CJ0 2.5 Å, Crystal structure of DNAJC9 HBD in complex with H3.3-H4 dimer and MCM2 HBD
- 7W1Y 2.59 Å, Human MCM double hexamer bound to natural DNA duplex (polyAT/polyTA)
- 9E2Z 2.6 Å, Cryo-EM structure of human CMG helicase stalled at G4-containing DNA template
- 5BNV 2.79 Å, Crystal structure of Human MCM2 HBD chaperoning a histone H3-H4 tetramer
- 7PLO 2.8 Å, H. sapiens replisome-CUL2/LRR1 complex
- 8W0F 2.8 Å, Cryo-EM structure of a human MCM2-7 double hexamer on dsDNA
- 4UUZ 2.9 Å, MCM2-histone complex
- 5BO0 2.91 Å, Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.2-H4 dimer
Browse structure collections
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