8WE6: PDB entry 8WE6

Human L-type voltage-gated calcium channel Cav1.2 at 2.9 Angstrom resolution. Determined by electron microscopy at 2.9 Å resolution. Released 6 Dec 2023.

Method: Electron microscopy
Resolution: 2.9 Å
Organism: Homo sapiens
Chains: 3
Atoms: 20,758
Mol. weight: 432.97 kDa
Ligands: CLR, 3PE, CA, PT5
Released: 6 Dec 2023

Explore 8WE6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8WE6 contains 129 α-helices and 71 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 67 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	126-142	17
α-helix	154-181	28
α-helix	194-216	23
α-helix	237-242	6
α-helix	245-250	6
α-helix	252-262	11
α-helix	266-268	3
α-helix	269-289	21
β-strand	296-298	3	1
β-strand	315	1	1
α-helix	324-326	3
β-strand	332-334	3	1
α-helix	351-360	10
α-helix	365-375	11
α-helix	382-389	8
α-helix	390-394	5
α-helix	395-418	24
α-helix	421-434	14
α-helix	437-443	7
α-helix	514-521	8
α-helix	528-541	14
α-helix	552-576	25
α-helix	587-607	21
α-helix	617-630	14
α-helix	635-646	12
α-helix	648-673	26
α-helix	684-685	2
α-helix	692-704	13
α-helix	708-718	11
α-helix	724-727	4
α-helix	731-747	17
α-helix	749-776	28
α-helix	903-913	11
α-helix	915-918	4
α-helix	929-974	26
α-helix	990-1006	17
α-helix	1014-1017	4
α-helix	1020-1023	4
α-helix	1025-1028	4
α-helix	1037-1048	12
α-helix	1052-1071	20
β-strand	1077-1078	2	2
α-helix	1086-1088	3
β-strand	1092-1096	5	3
α-helix	1097-1099	3
β-strand	1104-1108	5	3
β-strand	1111-1112	2	2
α-helix	1121-1132	12
α-helix	1137-1145	9
α-helix	1160-1162	3
α-helix	1163-1169	7
α-helix	1170-1177	8
α-helix	1178-1194	17
α-helix	1206-1214	9
α-helix	1228-1236	9
α-helix	1239-1255	17
α-helix	1265-1300	36
α-helix	1302-1321	20
α-helix	1378-1381	4
α-helix	1383-1388	6
α-helix	1394-1407	14
α-helix	1411-1430	20
α-helix	1450-1461	12
α-helix	1466-1471	6
β-strand	1477	1	4
β-strand	1495	1	4
α-helix	1499-1524	26
α-helix	1527-1530	4
α-helix	1539-1550	12
α-helix	1562-1566	5
α-helix	1569-1571	3
α-helix	1586-1593	8
α-helix	1606-1617	12
α-helix	1626-1632	7
α-helix	1639	1
α-helix	1645-1647	3

Chain C: 15 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	39-45	7
α-helix	47-51	5
α-helix	58-60	3
β-strand	64	1	21
β-strand	65	1	22
β-strand	93	1	21
β-strand	103-107	5	23
β-strand	115-119	5	23
α-helix	121-127	7
α-helix	130-132	3
α-helix	153-155	3
β-strand	172	1	22
β-strand	180-183	4	24
α-helix	192-198	7
α-helix	200-207	8
α-helix	247-250	4
α-helix	258-263	6
β-strand	288-291	4	24
α-helix	299-305	7
α-helix	311-313	3
α-helix	316-320	5
α-helix	323-326	4
β-strand	335-336	2	24
α-helix	342-358	17

Chain D: 47 helices, 53 β-strands

Element	Residues	Length	Sheet
α-helix	30-42	13
α-helix	45-50	6
α-helix	54-63	10
α-helix	65-67	3
β-strand	69-72	4	5
α-helix	75-110	36
β-strand	122-124	3	6
β-strand	125-126	2	7
β-strand	142-144	3	6
β-strand	150-151	2	8
β-strand	158-159	2	8
β-strand	160	1	9
β-strand	165-167	3	7
α-helix	173	1
α-helix	177-185	9
α-helix	189-199	11
β-strand	206-210	5	7
β-strand	215-218	4	7
β-strand	220	1	9
α-helix	238-240	3
α-helix	242-248	7
β-strand	252	1	10
β-strand	255-259	5	11
α-helix	262-264	3
α-helix	267-279	13
α-helix	280-282	3
β-strand	289	1	12
β-strand	290-294	5	11
β-strand	298-300	3	11
β-strand	308	1	12
α-helix	312-322	11
α-helix	333-343	11
β-strand	355	1	10
β-strand	357-362	6	11
α-helix	370-376	7
β-strand	383-389	7	11
α-helix	397-405	9
β-strand	410-413	4	11
α-helix	416-418	3
α-helix	419-422	4
α-helix	426-430	5
α-helix	432-437	6
α-helix	438-441	4
β-strand	445-446	2	7
β-strand	450-451	2	7
β-strand	458-467	10	7
β-strand	484-493	10	7
α-helix	494-499	6
β-strand	511-515	5	13
β-strand	520	1	14
β-strand	521-523	3	13
α-helix	531-533	3
β-strand	560	1	14
α-helix	561-564	4
α-helix	570-579	10
β-strand	584-593	10	13
α-helix	594	1
β-strand	599	1	15
β-strand	600-611	12	13
β-strand	618-624	7	13
β-strand	629-632	4	5
α-helix	640-645	6
α-helix	646-648	3
α-helix	650-652	3
α-helix	653-656	4
β-strand	658-659	2	16
α-helix	671-672	2
α-helix	676-683	8
α-helix	700-718	19
β-strand	732-736	5	17
β-strand	741	1	17
β-strand	742-743	2	16
α-helix	747-750	4
α-helix	758-760	3
α-helix	762-769	8
β-strand	774-776	3	17
β-strand	792-797	6	17
β-strand	800-801	2	18
β-strand	806-807	2	18
β-strand	810-816	7	17
α-helix	818-826	9
β-strand	848-854	7	19
β-strand	858	1	20
β-strand	859-861	3	19
α-helix	868-870	3
β-strand	874	1	20
α-helix	875-878	4
α-helix	880-888	9
β-strand	892-904	13	15
β-strand	972-983	12	15
β-strand	989-994	6	19
β-strand	1001-1008	8	19
β-strand	1013-1019	7	19
α-helix	1021-1023	3
α-helix	1028-1031	4
β-strand	1036-1038	3	15
α-helix	1040	1
α-helix	1042	1
α-helix	1043-1046	4
α-helix	1050-1052	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Voltage-dependent L-type calcium channel subunit alpha-1C	A	protein	2201	Homo sapiens	Q13936 (AlphaFold model)
Voltage-dependent calcium channel subunit alpha-2/delta-1	D	protein	1103	Homo sapiens	P54289 (AlphaFold model)
Voltage-dependent L-type calcium channel subunit beta-3	C	protein	484	Homo sapiens	P54284 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8WE6_1 Voltage-dependent L-type calcium channel subunit alpha-1C (chains A)
MVNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAAR
QAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISI
VEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIA
YGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFR
VLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYN
QEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCI
TMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGD
FQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVA
GGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLT
IASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGI
LETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFL
FIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYG
GPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLAR
TASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNP
ETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDT
IFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLH
KGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQ
CVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGE
VDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRV
EISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLR
RYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTG
LFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNHYFCDAWNTFDALIVV
GSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWT
FIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRC
ATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFV
AVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGK
LCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKI
WKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQ
AGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQS
DGRSAFPQTFTTQRPLHINKAGSSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANN
TALGRLPRPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRERHVPMCEDLELRRDSG
SAGTQAHCLLLRKANPSRCHSRESQAAMAGQEETSQDETYEVKMNHDTEACSEPSLLSTE
MLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRASFHLECLKRQKDRGGDISQK
TVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPPATPGSRGWPPQPVPTLRLEGV
ESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVSLMVPSQAGAPGRQFHGSASSLV
EAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMESAADNILSGGAPQSPNGALLPF
VNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRVYVSSL

Sequence of entity 2 (D), FASTA

>8WE6_2 Voltage-dependent calcium channel subunit alpha-2/delta-1 (chains D)
MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDI
YEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASN
EVVYYNAKDDLDPEKNDSEPGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVL
NELNWTSALDEVFKKNREEDPSLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRR
RPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQD
VSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIML
FTDGGEERAQEIFNKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR
INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQFENKTNLK
NQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTIN
LRKRRPNIQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYI
DKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARYSETLKPDNFEESGYTF
IAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNADLINRVLLDAGFTNELVQNYW
SKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYF
NKSGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCD
CKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAFNKSYDY
QSVCEPGAAPKQGAGHRSAYVPSVADILQIGWWATAAAWSILQQFLLSLTFPRLLEAVEM
EDDDFTASLSKQSCITEQTQYFFDNDSKSFSGVLDCGNCSRIFHGEKLMNTNLIFIMVES
KGTCPCDTRLLIQAEQTSDGPNPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPS
LWYIIGIQFLLLWLVSGSTHRLL

Sequence of entity 3 (C), FASTA

>8WE6_3 Voltage-dependent L-type calcium channel subunit beta-3 (chains C)
MYDDSYVPGFEDSEAGSADSYTSRPSLDSDVSLEEDRESARREVESQAQQQLERAKHKPV
AFAVRTNVSYCGVLDEECPVQGSGVNFEAKDFLHIKEKYSNDWWIGRLVKEGGDIAFIPS
PQRLESIRLKQEQKARRSGNPSSLSDIGNRRSPPPSLAKQKQKQAEHVPPYDVVPSMRPV
VLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADLSLAKRSVLNNPGKRTIIER
SSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHPAQLAKTSLAPIIVFVKVSSPKVL
QRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVILDENQLEDACEHLAEYLEVYWRAT
HHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQR
SSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWP
KDSY

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	4
3PE	1,2-Distearoyl-sn-glycerophosphoethanolamine	C41 H82 N O8 P	1
CA	Calcium ion	Ca	2
PT5	[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4…	C47 H85 O19 P3	1
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	3

Primary citation

Structural basis for human Ca v 1.2 inhibition by multiple drugs and the neurotoxin calciseptine. Gao, S., Yao, X., Chen, J. et al. Cell (2023) 186:5363-5374.e16. DOI 10.1016/j.cell.2023.10.007 · PubMed

Other PDB entries of the same protein (UniProt Q13936 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2F3Y 1.45 Å, Calmodulin/IQ domain complex
2F3Z 1.6 Å, Calmodulin/IQ-AA domain complex
6DAD 1.65 Å, 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex
6U3A 1.65 Å, 1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex
5V2Q 1.7 Å, CaV beta2a subunit: CaV1.2 AID-CEN complex
6U3B 1.7 Å, 1.7 Angstrom crystal structure of the Q135P Ca-CaM:CaV1.2 IQ domain complex
6U3D 1.75 Å, 1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex
1T0J 2.0 Å, Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and…
2BE6 2.0 Å, 2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex
5V2P 2.0 Å, CaV beta2a subunit: CaV1.2 AID-CAP complex
6DAE 2.0 Å, 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex
3G43 2.1 Å, Crystal structure of the calmodulin-bound Cav1.2 C-terminal regulatory domain dimer

Browse structure collections

About this viewer

MolViewer shows 8WE6 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.