8XGU: A peptide receptor complex structure

a peptide receptor complex structure. Determined by electron microscopy at 3.0 Å resolution. Released 30 Oct 2024.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, synthetic construct
Chains: 6
Atoms: 8,799
Mol. weight: 158.85 kDa
Released: 30 Oct 2024

Explore 8XGU in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8XGU contains 34 α-helices and 67 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 0 β-strands

Element	Residues	Length
α-helix	41-44	4
α-helix	46-69	24
α-helix	76-100	25
α-helix	112-145	34
α-helix	156-174	19
α-helix	175-178	4
α-helix	198-209	12
α-helix	210-214	5
α-helix	215-233	19
α-helix	247-253	7
α-helix	254-258	5
α-helix	259-275	17
α-helix	278-288	11
α-helix	299-324	26
α-helix	328-334	7

Chain B: 4 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	5-25	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-153	8	5
β-strand	156-161	6	5
β-strand	165-169	5	5
β-strand	176-181	6	5
β-strand	187	1	6
β-strand	191-192	2	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	220-223	4	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	249-254	6	7
β-strand	259-264	6	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain C: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-38	5	1
α-helix	48-51	4
β-strand	185-191	7	1
β-strand	194-200	7	1
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	1
α-helix	242-254	13
β-strand	263-269	7	1
α-helix	271-278	8
α-helix	283-285	3
α-helix	296-308	13
β-strand	319-323	5	1
α-helix	330-350	21

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-22	14
α-helix	30-43	14
α-helix	53-55	3

Chain S: 3 helices, 32 β-strands

Element	Residues	Length	Sheet
β-strand	6-7	2	9
β-strand	10-12	3	10
β-strand	17-23	7	9
α-helix	29-31	3
β-strand	33-35	3	11
β-strand	38-39	2	12
β-strand	45-46	2	12
β-strand	49	1	13
β-strand	50-51	2	11
β-strand	60	1	13
β-strand	65	1	14
β-strand	68	1	14
β-strand	69-73	5	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-94	3	10
β-strand	98-99	2	11
β-strand	115-119	5	10
β-strand	140-141	2	15
β-strand	147-148	2	16
β-strand	155-161	7	15
β-strand	166	1	17
β-strand	172	1	17
β-strand	174-175	2	18
β-strand	178-179	2	19
β-strand	185-186	2	19
β-strand	189-190	2	18
β-strand	194-195	2	18
β-strand	203-207	5	15
β-strand	211-216	6	15
β-strand	226-227	2	19
β-strand	230-231	2	18
α-helix	237	1
β-strand	243	1	19
β-strand	245-246	2	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
KiSS-1 receptor	A	protein	398	Homo sapiens	Q969F8 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	C	protein	351	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	357	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
scfv16	S	protein	261	Homo sapiens
TAK448	E	protein	11	synthetic construct

Sequence of entity 1 (A), FASTA

>8XGU_1 KiSS-1 receptor (chains A)
MHTVATSGPNASWGAPANASGCPGCGANASDGPVPSPRAVDAWLVPLFFAALMLLGLVGN
SLVIYVICRHKPMRTVTNFYIANLAATDVTFLLCCVPFTALLYPLPGWVLGDFMCKFVNY
IQQVSVQATCATLTAMSVDRWYVTVFPLRALHRRTPRLALAVSLSIWVGSAAVSAPVLAL
HRLSPGPRAYCSEAFPSRALERAFALYNLLALYLLPLLATCACYAAMLRHLGRVAVRPAP
ADSALQGQVLAERAGAVRAKVSRLVAAVVLLFAACWGPIQLFLVLQALGPAGSWHPRSYA
AYALKTWAHCMSYSNSALNPLLYAFLGSHFRQAFRRVCPCAPRRPRRPRRPGPSDPAAPH
AELLRLGSHPAPARAQKPGSSGLAARGLCVLGEDNAPL

Sequence of entity 2 (C), FASTA

>8XGU_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains C)
TLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSE
EECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAEL
AGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTG
IVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRM
HESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYI
QCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (B), FASTA

>8XGU_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRI
QMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTC
AYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFT
GHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGR
LLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>8XGU_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (S), FASTA

>8XGU_5 scfv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQGPAAA

Sequence of entity 6 (E), FASTA

>8XGU_6 TAK448 (chains E)
XYPNTFXLRWX

Primary citation

Structural basis for the ligand recognition and G protein subtype selectivity of kisspeptin receptor. Wu, Z., Chen, G., Qiu, C. et al. Sci Adv (2024) 10:eadn7771-eadn7771. DOI 10.1126/sciadv.adn7771 · PubMed

Other PDB entries of the same protein (UniProt Q969F8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8XGO 2.68 Å, a peptide receptor complex structure
8XGS 2.95 Å, a peptide receptor complex structure
8ZJD 3.06 Å, Cryo-EM structure of kisspeptin receptor bound to KP-10
8ZJE 3.07 Å, Cryo-EM structure of kisspeptin receptor bound to TAK-448
7YQE 3.5 Å, Structure of human SRC regulatory domains in complex with the C-terminal PRRP motifs of…

Browse structure collections

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