8ZJE: Kisspeptin receptor

Cryo-EM structure of kisspeptin receptor bound to TAK-448. Determined by electron microscopy at 3.07 Å resolution. Released 3 Jul 2024.

Method: Electron microscopy
Resolution: 3.07 Å
Organisms: Homo sapiens, Mus sp., synthetic construct
Chains: 6
Atoms: 8,743
Mol. weight: 158.15 kDa
Released: 3 Jul 2024

Explore 8ZJE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8ZJE contains 41 α-helices and 62 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-26	20
β-strand	33-38	6	1
α-helix	46-57	12
β-strand	192-197	6	1
β-strand	202-207	6	1
α-helix	219-222	4
β-strand	227-233	7	1
α-helix	234-236	3
β-strand	240	1	2
β-strand	248	1	2
α-helix	249-261	13
α-helix	264-266	3
β-strand	270-276	7	1
α-helix	278-285	8
α-helix	290-292	3
α-helix	304-316	13
β-strand	326-330	5	1
α-helix	338-357	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	11-30	20
α-helix	35-38	4
α-helix	43-44	2
β-strand	52-57	6	3
β-strand	63-68	6	4
β-strand	74-79	6	4
β-strand	83-88	6	4
β-strand	93-99	7	4
β-strand	105-110	6	5
β-strand	116-121	6	5
β-strand	126-130	5	5
β-strand	139-144	6	5
β-strand	151-156	6	6
β-strand	161-166	6	6
β-strand	171-175	5	6
β-strand	180-185	6	6
β-strand	192-197	6	7
β-strand	203-208	6	7
β-strand	212-217	6	7
β-strand	223-228	6	7
β-strand	236-239	4	8
β-strand	245-249	5	8
β-strand	255-259	5	8
β-strand	264-269	6	8
β-strand	278-283	6	9
β-strand	289-294	6	9
β-strand	299-303	5	9
β-strand	309-312	4	9
β-strand	320-325	6	3
β-strand	332-336	5	3
β-strand	340-344	5	3

Chain G: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	6-23	18
α-helix	29-43	15
α-helix	44-46	3
α-helix	52-54	3
α-helix	55-57	3

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	44-67	24
α-helix	76-94	19
α-helix	98-102	5
α-helix	112-145	34
α-helix	156-173	18
α-helix	176-179	4
β-strand	181-183	3	10
β-strand	191-193	3	10
α-helix	198-208	11
α-helix	209-214	6
α-helix	215-233	19
α-helix	243-248	6
α-helix	249-254	6
α-helix	255-288	34
α-helix	299-320	22
α-helix	321-325	5
α-helix	328-335	8

Chain S: 8 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	2-5	4	11
α-helix	6-7	2
β-strand	9-11	3	12
β-strand	16-24	9	11
α-helix	28-30	3
β-strand	32-38	7	12
β-strand	44-50	7	12
α-helix	52-54	3
β-strand	57-59	3	12
β-strand	67-72	6	11
α-helix	73-75	3
β-strand	77-83	7	11
α-helix	87-89	3
β-strand	91-98	8	12
β-strand	110	1	12
β-strand	114-118	5	12
α-helix	137-139	3
β-strand	140-142	3	13
β-strand	146-148	3	14
β-strand	155-161	7	13
β-strand	166	1	15
β-strand	172	1	15
β-strand	174-179	6	14
β-strand	186-189	4	14
β-strand	195	1	14
α-helix	196	1
β-strand	203-207	5	13
β-strand	211-216	6	13
α-helix	221-223	3
β-strand	226-231	6	14
β-strand	239	1	14
β-strand	243-246	4	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q)…	A	protein	353	Homo sapiens	P50148 (AlphaFold model), P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
KiSS-1 receptor	R	protein	398	Homo sapiens	Q969F8
scFv16	S	protein	247	Mus sp.
Ace-dty-hyp-asn-thr-phe-xza-leu-nmm-trp-NH2	C	protein	11	synthetic construct

Sequence of entity 1 (A), FASTA

>8ZJE_1 Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGERSRRELKLLLLGTGESGKSTFIKQMRIIHGSG
YSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFEN
PYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPT
TGIIEYPFDLQSVIFRMVDVGAQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCSTDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 2 (B), FASTA

>8ZJE_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>8ZJE_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (R), FASTA

>8ZJE_4 KiSS-1 receptor (chains R)
MHTVATSGPNASWGAPANASGCPGCGANASDGPVPSPRAVDAWLVPLFFAALMLLGLVGN
SLVIYVICRHKPMRTVTNFYIANLAATDVTFLLCCVPFTALLYPLPGWVLGDFMCKFVNY
IQQVSVQATCWTLTAMSVDRWYVTVFPLRALHRRTPRLALAVSLSIWVGSAAVSAPVLAL
HRLSPGPRAYCSEAFPSRALERAFALYNLLALYLLPLLATCACYAAMLRHLGRVAVRPAP
ADSALQGQVLAERAGAVRAKVSRLVAAVVLLFAACWGPIQLFLVLQALGPAGSWHPRSYA
AYALKTWAHCMSYSNSALNPLLYAFLGSHFRQAFRRVCPCAPRRPRRPRRPGPSDPAAPH
AELLRLGSHPAPARAQKPGSSGLAARGLCVLGEDNAPL

Sequence of entity 5 (S), FASTA

>8ZJE_5 scFv16 (chains S)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL

Sequence of entity 6 (C), FASTA

>8ZJE_6 ACE-DTY-HYP-ASN-THR-PHE-XZA-LEU-NMM-TRP-NH2 (chains C)
XYPNTFXLRWX

Primary citation

Structural basis for hormone recognition and distinctive Gq protein coupling by the kisspeptin receptor. Shen, S., Wang, D., Liu, H. et al. Cell Rep (2024) 43:114389-114389. DOI 10.1016/j.celrep.2024.114389 · PubMed

Other PDB entries of the same protein (UniProt P50148 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9VAW 2.24 Å, Cryo-EM structure of human Neurotensin Receptor 1 (hNTSR1)-Gq (delipidated) complex in…
9M1H 2.55 Å, Cryo-EM structure of PGE2-EP1-Gq complex
9XIX 2.58 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the C-state
8THK 2.6 Å, Cryo-EM structure of A61603-bound alpha-1A-adrenergic receptor in complex with…
9XH2 2.6 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the E-state
8G59 2.64 Å, Cryo-EM structure of the TUG891 bound GPR120-Giq complex
24XB 2.65 Å, Cryo-EM Structure of the Apo FFAR4-Gq Complex
8XGO 2.68 Å, a peptide receptor complex structure
9K27 2.68 Å, PrRP31 bound prolactin-releasing peptide receptor coupled with Gq protein complex
9XJP 2.74 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the E-state, chemokine domain fully…
9LL9 2.76 Å, DOI-bound Serotonin 2A (5-HT2A) receptor-Gq complex
7F6I 2.8 Å, Cryo-EM structure of human bradykinin receptor BK2R in complex Gq proteins and kallidin

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