9BIP: Human proton sensing receptor GPR4

Human proton sensing receptor GPR4 in complex with miniGs. Determined by electron microscopy at 2.8 Å resolution. Released 15 Jan 2025.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Homo sapiens, Lama glama
Chains: 5
Atoms: 8,325
Mol. weight: 136.17 kDa
Released: 15 Jan 2025

Explore 9BIP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9BIP contains 38 α-helices and 49 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	10-18	9
α-helix	19-23	5
α-helix	24-37	14
β-strand	40-46	7	1
α-helix	53-63	11
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	234-238	5
β-strand	243-248	6	1
α-helix	253-265	3
α-helix	266-277	12
β-strand	286-291	6	1
α-helix	294-303	10
α-helix	308-310	3
α-helix	313-317	5
α-helix	332-351	20
β-strand	359-363	5	1
α-helix	371-390	20

Chain B: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	2-25	24
α-helix	30-34	5
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	306-307	2	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain G: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	6-22	17
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3
α-helix	56-58	3

Chain N: 2 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	11-12	2	10
β-strand	17-25	9	9
β-strand	34-39	6	11
β-strand	46-51	6	11
β-strand	58-60	3	11
α-helix	62-64	3
β-strand	69-73	5	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-98	7	11
β-strand	118	1	11
β-strand	122-124	3	11
β-strand	125-126	2	10

Chain R: 17 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	19-47	29
α-helix	52-67	16
α-helix	70-78	9
β-strand	82	1	12
α-helix	87-120	34
α-helix	131-149	19
α-helix	151-154	4
β-strand	158-160	3	12
β-strand	166-168	3	12
α-helix	174-175	2
α-helix	176-186	11
α-helix	187-191	5
α-helix	192-208	17
α-helix	216-253	38
α-helix	259-263	5
α-helix	265-274	10
α-helix	275-277	3
α-helix	278-283	6
α-helix	284-288	5
α-helix	293-295	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
miniGs	A	protein	261	Homo sapiens	A0A804HIH4 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	370	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	68	Homo sapiens	P59768 (AlphaFold model)
Nb35	N	protein	142	Lama glama
G-protein coupled receptor 4	R	protein	373	Homo sapiens	P46093 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9BIP_1 miniGs (chains A)
GGSLEVLFQGPSGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSG
KSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTA
IIFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFP
EFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENAR
RIFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>9BIP_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPEDQVDPRLIDGKGSSGSELDQLRQEAEQLKNQIRDARKACADATL
SQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKV
HAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCR
FLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKL
WDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNII
CGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATG
SWDSFLKIWN

Sequence of entity 3 (G), FASTA

>9BIP_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFC

Sequence of entity 4 (N), FASTA

>9BIP_4 Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSGSEDQVDPRLIDGK

Sequence of entity 5 (R), FASTA

>9BIP_5 G-protein coupled receptor 4 (chains R)
DYKDDDDASIDMGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQR
NELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLC
CISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCF
EKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSL
IAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLV
NEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQ
GDQVQLKMLPPAQ

Primary citation

Molecular basis of proton sensing by G protein-coupled receptors. Howard, M.K., Hoppe, N., Huang, X.P. et al. Cell (2025) 188:671-687.e20. DOI 10.1016/j.cell.2024.11.036 · PubMed

Other PDB entries of the same protein (UniProt A0A804HIH4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9GGG 3.25 Å, Cryo-EM structure of Thromboxane A2 receptor-miniGq Protein Complex bound to I-BOP
9JFT 3.27 Å, Cryo-EM structure of GPR65 complexed with miniGs in pH6.5
9K25 3.31 Å, Cryo-EM structure of apo-P2Y purinoceptor 2-miniGq-Nb35 complex

Browse structure collections

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