9F6Y: Human Mediator subunit MED23

CryoEM structure of Human Mediator subunit MED23 complexed with phosphorylated Elk-1 transcription factor. Determined by electron microscopy at 2.98 Å resolution. Released 30 Apr 2025.

Method

Electron microscopy

Resolution

2.98 Å

Organism

Homo sapiens

Chains

2

Atoms

10,065

Mol. weight

195.92 kDa

Released

30 Apr 2025

Explore 9F6Y in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9F6Y contains 87 α-helices and 0 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 87 helices, 0 β-strands

Molecules and chains

>9F6Y_1 Mediator of RNA polymerase II transcription subunit 23 (chains A)
METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCI
QWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFK
LVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPA
YFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNS
WKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVL
EDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKL
AGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQST
HAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNA
YSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLI
HSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGI
LHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSE
VQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIM
SFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHF
SMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGG
QQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRV
SDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVC
LRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRA
FLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMA
GKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRE
NITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSY
SEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQ
QERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICN
LKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQDVLFQGPGHHHH
HH

>9F6Y_2 Green fluorescent protein,ETS domain-containing protein Elk-1 (chains B)
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPT
LVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL
VNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLA
DHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKG
SSQPQKGRKPRDLELPLSPSLLGGPGPERAPGSGSGSGLQAPGPALAPSLLPTHTLAPVL
LTPSSLPPSIHFWSTLSPIAPRSPAKLSFQFPSSGHHHHHH

Primary citation

Structural basis of human Mediator recruitment by the phosphorylated transcription factor Elk-1. Monte, D., Lens, Z., Dewitte, F. et al. Nat Commun (2025) 16:3772-3772. DOI 10.1038/s41467-025-59014-8 · PubMed

Related structures

Other PDB entries of the same protein (UniProt Q9ULK4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 6H02 2.8 Å, Crystal structure of human Mediator subunit MED23
• 9F76 3.1 Å, CryoEM structure of human Mediator subunit Med23
• 7EMF 3.5 Å, Human Mediator (deletion of MED1-IDR) in a Tail-extended conformation
• 8TRH 3.7 Å, The IDRc bound human core Mediator complex
• 7ENA 4.07 Å, TFIID-based PIC-Mediator holo-complex in pre-assembled state (pre-hPIC-MED)
• 7ENC 4.13 Å, TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)
• 8GXS 4.16 Å, PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
• 7ENJ 4.4 Å, Human Mediator (deletion of MED1-IDR) in a Tail-bent conformation (MED-B)
• 8T9D 4.66 Å, CryoEM structure of TR-TRAP
• 7LBM 4.8 Å, Structure of the human Mediator-bound transcription pre-initiation complex
• 8GXQ 5.04 Å, PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state
• 8TQW 8.2 Å, Structure of human transcriptional Mediator complex

Browse structure collections

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