9HNP: PTS system glucose-specific EIICB component

Cryo-EM structure of the glucose-specific PTS transporter IICB from E. coli in an intermediate state. Determined by electron microscopy at 2.53 Å resolution. Released 2 Apr 2025.

Method: Electron microscopy
Resolution: 2.53 Å
Organism: Escherichia coli
Chains: 2
Atoms: 5,924
Mol. weight: 105.43 kDa
Ligands: LMT
Released: 2 Apr 2025

Explore 9HNP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9HNP contains 48 α-helices and 10 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and B: 24 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	5-34	30
α-helix	42-57	16
α-helix	59-70	12
β-strand	75	1	1
α-helix	76-101	26
α-helix	105-111	7
α-helix	117-135	19
α-helix	142-147	6
α-helix	149-151	3
α-helix	152-184	33
α-helix	185-189	5
α-helix	191-205	15
α-helix	206-208	3
α-helix	211-220	10
β-strand	224-226	3	4
β-strand	232-234	3	4
α-helix	236-241	6
α-helix	249-252	4
α-helix	253-254	2
α-helix	255-260	6
α-helix	261-271	11
α-helix	274-294	21
α-helix	298-304	7
α-helix	309-329	21
β-strand	332	1	5
α-helix	341-346	6
α-helix	348-350	3
β-strand	352	1	5
α-helix	356-378	23

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
PTS system glucose-specific EIICB component	A, B	protein	485	Escherichia coli	P69786 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>9HNP_1 PTS system glucose-specific EIICB component (chains A, B)
MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMP
LIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLG
GIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVVLSFIWPPIGSAIQ
TFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRY
MAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPI
EFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIV
GIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSEMAPALVAAFGGKENITNLDA
CITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNHLEL
EVLFQ

Ligands and cofactors

ID	Name	Formula	Copies
LMT	Dodecyl-beta-D-maltoside	C24 H46 O11	4

Primary citation

Cryo-EM structure of a phosphotransferase system glucose transporter stalled in an intermediate conformation. Roth, P., Fotiadis, D. J Struct Biol X (2025) 11:100124-100124. DOI 10.1016/j.yjsbx.2025.100124 · PubMed

Other PDB entries of the same protein (UniProt P69786 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3BP3 1.65 Å, Crystal structure of EIIB
8QSR 2.56 Å, Cryo-EM structure of the glucose-specific PTS transporter IICB from E. coli in the…
3BP8 2.85 Å, Crystal structure of Mlc/EIIB complex
8QST 2.89 Å, Cryo-EM structure of the glucose-specific PTS transporter IICB from E. coli in the…
9SRX 2.96 Å, Cryo-EM structure of the Mlc repressor in complex with the glucose-specific IICB…
1IBA Glucose permease (domain iib), NMR, 11 structures
1O2F Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli…
9A3B Model of E. coli PtsG by in-cell photo-crosslinking MS and deep learning

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