9HVF: Native human PPP1R15B-P-eIF2-eIF2B complex

Native human PPP1R15B-P-eIF2-eIF2B complex. Determined by electron microscopy at 3.8 Å resolution. Released 19 Nov 2025.

Method: Electron microscopy
Resolution: 3.8 Å
Organism: Homo sapiens
Chains: 5
Atoms: 8,225
Mol. weight: 188.03 kDa
Released: 19 Nov 2025

Explore 9HVF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9HVF contains 34 α-helices and 59 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 5 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	189-199	11	18
α-helix	205-215	11
α-helix	216-218	3
β-strand	225-231	7	18
β-strand	234-241	8	18
α-helix	244-263	20
β-strand	267-277	11	18
α-helix	280-297	18
α-helix	299	1
β-strand	300-301	2	13

Chain C: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	10-22	13

Chain D: 13 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	9	1	11
α-helix	16-18	3
α-helix	33-36	4
β-strand	42-47	6	12
α-helix	54-61	8
β-strand	67-68	2	13
β-strand	82-88	7	12
α-helix	90-92	3
β-strand	105	1	12
β-strand	113-114	2	14
α-helix	115	1
β-strand	123-124	2	14
β-strand	128-134	7	12
α-helix	138-146	9
β-strand	154-157	4	12
α-helix	168-180	13
β-strand	185-190	6	12
α-helix	197-211	15
β-strand	221-224	4	12
α-helix	233-241	9
β-strand	255-262	8	15
α-helix	271-273	3
β-strand	278-285	8	15
β-strand	295	1	15
β-strand	315	1	15
β-strand	318-322	5	15
β-strand	323	1	16
β-strand	326	1	16
β-strand	328	1	11
β-strand	337-341	5	15
α-helix	345-348	4
β-strand	358-359	2	15
β-strand	370-377	8	17
α-helix	396-399	4
β-strand	403-407	5	17
β-strand	412-420	9	17
β-strand	424-428	5	17
β-strand	440-447	8	17
β-strand	450-463	14	17
α-helix	464-466	3

Chain E: 15 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	1
α-helix	16-18	3
α-helix	30-32	3
α-helix	37-46	10
β-strand	51	1	2
β-strand	52-55	4	1
α-helix	57-62	6
β-strand	73	1	2
β-strand	76-77	2	1
α-helix	86-92	7
β-strand	101-104	4	1
β-strand	108-110	3	3
α-helix	115-124	10
β-strand	129-132	4	4
β-strand	133-134	2	5
α-helix	135-136	2
β-strand	157-160	4	6
β-strand	166	1	7
β-strand	167-171	5	6
α-helix	185-188	4
β-strand	196-197	2	6
β-strand	199-200	2	5
β-strand	205-206	2	4
α-helix	209-217	9
α-helix	224-228	5
α-helix	229-235	7
α-helix	240-243	4
α-helix	263-265	3
β-strand	301	1	7
β-strand	302-304	3	4
β-strand	310-312	3	3
α-helix	317-325	9
α-helix	328-333	6
β-strand	345	1	8
β-strand	362-363	2	8
β-strand	374	1	9
β-strand	379-380	2	8
β-strand	391	1	9
β-strand	397	1	8
β-strand	403	1	10
β-strand	414	1	8
β-strand	420	1	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Translation initiation factor eIF-2B subunit gamma	E	protein	452	Homo sapiens	Q9NR50 (AlphaFold model)
Protein phosphatase 1 regulatory subunit 15B	A	protein	109	Homo sapiens	Q5SWA1 (AlphaFold model)
Eukaryotic translation initiation factor 2 subunit 2	C	protein	333	Homo sapiens	P20042 (AlphaFold model)
Eukaryotic translation initiation factor 2 subunit 3	D	protein	472	Homo sapiens	P41091 (AlphaFold model)
Eukaryotic translation initiation factor 2 subunit 1	B	protein	315	Homo sapiens	P05198

Sequence of entity 1 (E), FASTA

>9HVF_1 Translation initiation factor eIF-2B subunit gamma (chains E)
MEFQAVVMAVGGGSRMTDLTSSIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVVTTRDVQ
KALCAEFKMKMKPDIVCIPDDADMGTADSLRYIYPKLKTDVLVLSCDLITDVALHEVVDL
FRAYDASLAMLMRKGQDSIEPVPGQKGKKKAVEQRDFIGVDSTGKRLLFMANEADLDEEL
VIKGSILQKHPRIRFHTGLVDAHLYCLKKYIVDFLMENGSITSIRSELIPYLVRKQFSSA
SSQQGQEEKEEDLKKKELKSLDIYSFIKEANTLNLAPYDACWNACRGDRWEDLSRSQVRC
YVHIMKEGLCSRVSTLGLYMEANRQVPKLLSALCPEEPPVHSSAQIVSKHLVGVDSLIGP
ETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTVEEGSNIQGSVICNNAVIEKGAD
IKDCLIGSGQRIEAKAKRVNEVIVGNDQLMEI

Sequence of entity 2 (A), FASTA

>9HVF_2 Protein phosphatase 1 regulatory subunit 15B (chains A)
DYKDDDDKGDLPISARPACSNKLIDYILGGASSDLETSSDPEGEDWDEEAEDDGFDSDSS
LSDSDLEQDPEGLHLWNSFCSVDPYNPQNFTATIQTAARIVPEEPSDSE

Sequence of entity 3 (C), FASTA

>9HVF_3 Eukaryotic translation initiation factor 2 subunit 2 (chains C)
MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEED
TRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIM
LGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELL
NRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFL
LAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFL
QCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Sequence of entity 4 (D), FASTA

>9HVF_4 Eukaryotic translation initiation factor 2 subunit 3 (chains D)
MAGGEAGVTLGQPHLSRQDLTTLDVTKLTPLSHEVISRQATINIGTIGHVAHGKSTVVKA
ISGVHTVRFKNELERNITIKLGYANAKIYKLDDPSCPRPECYRSCGSSTPDEFPTDIPGT
KGNFKLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIM
KLKHILILQNKIDLVKESQAKEQYEQILAFVQGTVAEGAPIIPISAQLKYNIEVVCEYIV
KKIPVPPRDFTSEPRLIVIRSFDVNKPGCEVDDLKGGVAGGSILKGVLKVGQEIEVRPGI
VSKDSEGKLMCKPIFSKIVSLFAEHNDLQYAAPGGLIGVGTKIDPTLCRADRMVGQVLGA
VGALPEIFTELEISYFLLRRLLGVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAV
KADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTVDDD

Sequence of entity 5 (B), FASTA

>9HVF_5 Eukaryotic translation initiation factor 2 subunit 1 (chains B)
MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSIN
KLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLE
YTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNI
NRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTT
TLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEV
DGDDDAEEMEAKAED

Primary citation

Termination of the integrated stress response. De Miguel, C., Thorkelsson, S.R., Fatalska, A. et al. Science (2026) 391:eadw5137-eadw5137. DOI 10.1126/science.adw5137 · PubMed

Other PDB entries of the same protein (UniProt Q9NR50 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9Y4B 2.1 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
7VLK 2.27 Å, eIF2B-SFSV NSs C2-imposed
9Y3U 2.4 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
9Y4W 2.4 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
7F64 2.42 Å, eIF2B-SFSV NSs
9Y3T 2.5 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
7RLO 2.6 Å, Structure of the human eukaryotic translation initiation factor 2B (eIF2B) in complex…
9HVE 2.7 Å, Native human eIF2-eIF2B complex
7F66 2.76 Å, eIF2B-SFSV NSs-1-eIF2
9Y5T 2.78 Å, eIF2B lacking the latch helix bound to ISRACT-02 (Active state)
6CAJ 2.8 Å, Electron cryo-microscopy of the eukaryotic translation initiation factor 2B from Homo…
7L70 2.8 Å, The eukaryotic translation initiation factor 2B from Homo sapiens in its apo form

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