9IJE: PDB entry 9IJE

Epinephrine-activated human beta3 adrenergic receptor. Determined by electron microscopy at 2.34 Å resolution. Released 21 Aug 2024.

Method: Electron microscopy
Resolution: 2.34 Å
Organisms: Rattus norvegicus, Lama glama, Homo sapiens
Chains: 6
Atoms: 9,340
Mol. weight: 161.39 kDa
Ligands: ALE
Released: 21 Aug 2024

Explore 9IJE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9IJE contains 38 α-helices and 76 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	14-38	25
β-strand	40-46	7	20
β-strand	207	1	21
β-strand	210-212	3	20
α-helix	214-216	3
β-strand	218-222	5	20
β-strand	224	1	21
α-helix	234-238	5
β-strand	243-248	6	20
α-helix	256-268	13
β-strand	276-281	6	20
α-helix	284-293	10
α-helix	298-301	4
α-helix	303-307	5
α-helix	316-317	2
α-helix	322-339	18
β-strand	349-353	5	20
α-helix	361-380	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	2-23	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	89-94	6	2
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
β-strand	134-139	6	3
β-strand	146-153	8	4
β-strand	156-161	6	4
β-strand	165-170	6	4
β-strand	175-181	7	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	208-212	5	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	250-254	5	6
β-strand	259-264	6	6
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	303-308	6	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	11-22	12
α-helix	30-43	14
α-helix	53-55	3

Chain D: 5 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	11-12	2	12
β-strand	18-25	8	11
α-helix	29-31	3
β-strand	33-39	7	13
β-strand	45-51	7	13
β-strand	58-60	3	13
β-strand	68-73	6	11
α-helix	74-76	3
β-strand	78-83	6	11
α-helix	88-90	3
β-strand	92-94	3	14
β-strand	95-99	5	13
β-strand	110-111	2	13
β-strand	115-117	3	14
β-strand	118-119	2	12
α-helix	138-139	2
β-strand	140-141	2	15
β-strand	147-148	2	16
β-strand	155-159	5	17
β-strand	160-161	2	15
β-strand	166	1	18
β-strand	172	1	18
β-strand	174-179	6	19
β-strand	185-190	6	19
β-strand	194-195	2	19
α-helix	196	1
β-strand	203-208	6	17
β-strand	211-216	6	17
β-strand	225	1	16
β-strand	226-231	6	19
β-strand	239	1	19
β-strand	245-246	2	16

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	8
β-strand	11-12	2	9
β-strand	18-25	8	8
α-helix	29-31	3
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	58-60	3	10
α-helix	62-64	3
β-strand	68-73	6	8
β-strand	78-83	6	8
α-helix	88-90	3
β-strand	92-98	7	10
β-strand	118	1	10
β-strand	122-124	3	10
β-strand	125-126	2	9

Chain R: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	43-64	22
α-helix	72-89	18
α-helix	91-100	10
α-helix	107-140	34
α-helix	145-148	4
α-helix	151-174	24
α-helix	203-209	7
α-helix	210-214	5
α-helix	215-242	28
α-helix	290-316	27
α-helix	320-322	3
α-helix	325-336	12
α-helix	338-346	9
α-helix	350-361	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Rattus norvegicus	P54311 (AlphaFold model)
Camelid antibody VHH fragment	N	protein	128	Lama glama
Single-chain Fv16	D	protein	246	Homo sapiens
Beta-3 adrenergic receptor	R	protein	321	Homo sapiens	P13945 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	373	Bos taurus	P04896 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	52	Bos taurus	P63212 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>9IJE_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
QSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (N), FASTA

>9IJE_2 Camelid antibody VHH fragment (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSS

Sequence of entity 3 (D), FASTA

>9IJE_3 Single-chain Fv16 (chains D)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSS
GGGGSGGGGSGGGGADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRP
GQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGA
GTKLEL

Sequence of entity 4 (R), FASTA

>9IJE_4 Beta-3 adrenergic receptor (chains R)
ALLALAVLATVGGNLLVIVAIAWTPRLQTMTNVFVTSLAAADLVMGLLVVPPAATLALTG
HWPLGATGCELWTSVDVLCVTASIWTLCALAVDRYLAVTNPLRYGALVTKRCARTAVVLV
WVVSAAVSFAPIMSQWWRVGADAEAQRCHSNPRCCAFASNMPYVLLSSSVSFYLPLLVML
FVYARVFVVATRQLRLLRGELGRFPPEESPPAPSRSLAPAPVGTCAPPEGVPACGRRPAR
LLPLREHRALCTLGLIMGTFTLCWLPFFLANVLRALGGPSLVPGPAFLALNWLGYANSAF
NPLIYCRSPDFRSAFRRLLCR

Sequence of entity 5 (A), FASTA

>9IJE_5 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
LSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHVNGFNGE
GGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYI
LSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYV
PSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSS
DYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTP
EDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCRD
IIQRMHLRQYELL

Sequence of entity 6 (C), FASTA

>9IJE_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
AQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF

Ligands and cofactors

ID	Name	Formula	Copies
ALE	L-epinephrine	C9 H13 N O3	1

Primary citation

Molecular Mechanism of the beta 3 AR Agonist Activity of a beta-Blocker. Zheng, S., Zhang, S., Dai, S. et al. Chempluschem (2024) 89:e202400288-e202400288. DOI 10.1002/cplu.202400288 · PubMed

Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF
7DUQ 2.5 Å, Cryo-EM structure of the compound 2 and GLP-1-bound human GLP-1 receptor-Gs complex

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