9JLC: MAS1-Gi Complex with AVE 0991
Cryo-EM structure of MAS1-Gi Complex with AVE 0991. Determined by electron microscopy at 2.98 Å resolution. Released 24 Sept 2025.
- Method
- Electron microscopy
- Resolution
- 2.98 Å
- Organism
- Homo sapiens
- Chains
- 5
- Atoms
- 8,247
- Mol. weight
- 139.91 kDa
- Ligands
- A1ECD
- Released
- 24 Sept 2025
Explore 9JLC in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9JLC contains 30 α-helices and 58 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-28 | 22 | |
| β-strand | 32-36 | 5 | 1 |
| α-helix | 44-50 | 7 | |
| β-strand | 70-74 | 5 | 1 |
| β-strand | 77-83 | 7 | 1 |
| α-helix | 91-93 | 3 | |
| α-helix | 95-98 | 4 | |
| β-strand | 106-108 | 3 | 2 |
| α-helix | 116-127 | 12 | |
| α-helix | 136-137 | 2 | |
| β-strand | 139-141 | 3 | 2 |
| α-helix | 144-153 | 10 | |
| α-helix | 156-158 | 3 | |
| α-helix | 169-181 | 13 | |
| β-strand | 195-196 | 2 | 2 |
| α-helix | 204-224 | 21 | |
Chain B: 4 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-24 | 21 | |
| α-helix | 30-34 | 5 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 3 |
| β-strand | 58-63 | 6 | 4 |
| β-strand | 69-74 | 6 | 4 |
| β-strand | 78-83 | 6 | 4 |
| β-strand | 89-94 | 6 | 4 |
| α-helix | 95 | 1 | |
| β-strand | 100-105 | 6 | 5 |
| β-strand | 111-116 | 6 | 5 |
| β-strand | 121-125 | 5 | 5 |
| β-strand | 134-139 | 6 | 5 |
| β-strand | 146-151 | 6 | 6 |
| β-strand | 156-161 | 6 | 6 |
| β-strand | 165-170 | 6 | 6 |
| β-strand | 175-181 | 7 | 6 |
| β-strand | 191-192 | 2 | 7 |
| β-strand | 198-202 | 5 | 7 |
| β-strand | 208-212 | 5 | 7 |
| β-strand | 218-222 | 5 | 7 |
| β-strand | 229-234 | 6 | 8 |
| β-strand | 240-245 | 6 | 8 |
| β-strand | 250-254 | 5 | 8 |
| β-strand | 260-264 | 5 | 8 |
| β-strand | 273-278 | 6 | 9 |
| β-strand | 284-289 | 6 | 9 |
| β-strand | 294-298 | 5 | 9 |
| β-strand | 304-308 | 5 | 9 |
| β-strand | 315-320 | 6 | 3 |
| β-strand | 327-331 | 5 | 3 |
| β-strand | 336-339 | 4 | 3 |
Chain G: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-23 | 14 | |
| α-helix | 30-43 | 14 | |
| α-helix | 45-47 | 3 | |
Chain R: 11 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 34-59 | 26 | |
| α-helix | 65-95 | 31 | |
| α-helix | 103-129 | 27 | |
| α-helix | 132-135 | 4 | |
| α-helix | 137-142 | 6 | |
| α-helix | 148-169 | 22 | |
| α-helix | 182-213 | 32 | |
| α-helix | 224-241 | 18 | |
| α-helix | 243-254 | 12 | |
| α-helix | 262-277 | 16 | |
| α-helix | 278-282 | 5 | |
Chain S: 2 helices, 24 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 10 |
| β-strand | 10-12 | 3 | 11 |
| β-strand | 17-25 | 9 | 10 |
| α-helix | 29-31 | 3 | |
| β-strand | 33-39 | 7 | 11 |
| β-strand | 45-51 | 7 | 11 |
| β-strand | 60 | 1 | 11 |
| β-strand | 68-73 | 6 | 10 |
| β-strand | 78-84 | 7 | 10 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-99 | 8 | 11 |
| β-strand | 111 | 1 | 11 |
| β-strand | 115-119 | 5 | 11 |
| β-strand | 143 | 1 | 12 |
| β-strand | 148-150 | 3 | 13 |
| β-strand | 157-162 | 6 | 12 |
| β-strand | 168 | 1 | 14 |
| β-strand | 174 | 1 | 14 |
| β-strand | 176-181 | 6 | 13 |
| β-strand | 188-192 | 5 | 13 |
| β-strand | 196-197 | 2 | 13 |
| β-strand | 205-208 | 4 | 12 |
| β-strand | 213-218 | 6 | 12 |
| β-strand | 228-233 | 6 | 13 |
| β-strand | 241 | 1 | 13 |
| β-strand | 245-248 | 4 | 13 |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(i) subunit alpha-1 | A | protein | 340 | Homo sapiens | P63096 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 338 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 53 | Homo sapiens | P59768 (AlphaFold model) |
| Proto-oncogene Mas | R | protein | 253 | Homo sapiens | P04201 (AlphaFold model) |
| scFv16 | S | protein | 259 | Homo sapiens | |
Sequence of entity 1 (A), FASTA
>9JLC_1 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
LSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGADNSGKSTIVKQMKIIHEAGYSEE
ECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELA
GVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGI
VETHFTFKDLHFKMFDVGGQRSERKKWIHCFEDVAAIIFCVDLSDYNRMHESMKLFDSIC
NNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYQEYAGSNTYEEAAAYIQCQFEDLNKRK
DTKEIYTHFTCATDTKNAQFIFDAVTDVIIKNNLKDCGLF
Sequence of entity 2 (B), FASTA
>9JLC_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
ELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMH
WGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICS
IYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTG
HTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFA
TGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKA
DRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>9JLC_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
IAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
Sequence of entity 4 (R), FASTA
>9JLC_4 Proto-oncogene Mas (chains R)
QIPIVHWVIMSISPVGFVENGILLWFLCFRMRRNPFTVYITHLSIADISLLFCIFILSID
YALDYELSSGHYYTIVTLSVTFLFGYNTGLYLLTAISVERCLSVLYPIWYRCHRPKYQSA
LVCALLWALSCLVTTMEYVMCIDREEESHSRNDCRAVIIFIAILSFLVFTPLMLVSSTIL
VVKIRKNTWASHSSKLYIVIMVTIIIFLIFAMPMRLLYLLYYEYWSTFGNLHHISLLFST
INSSANPFIYFFV
Sequence of entity 5 (S), FASTA
>9JLC_5 scFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAHHHHHHHH
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| A1ECD | 1-ethyl-3-[3-[4-[(5-methanoyl-4-methoxy-2-phenyl-imidazol-1-yl)methyl]phenyl]-5… | C29 H32 N4 O5 S2 | 1 |
Primary citation
Cryo-EM structure of MAS1-Gi complex. Wong, T.S., Xiong, T.T., Zeng, Z.C. To be published.
Other PDB entries of the same protein (UniProt P63096 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
- 3UMR 2.04 Å, Crystal structure of the G202D mutant of human G-alpha-i1
- 9P7Z 2.1 Å, NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
- 2OM2 2.2 Å, Crystal Structure Of Human G[alpha]i1 Bound To The Goloco Motif Of Rgs14
- 8YN9 2.3 Å, Cryo-EM structure of histamine H4 receptor in complex with histamine and Gi
- 9HYI 2.3 Å, DP81-bound serotonin 5-HT1A receptor - Gi Protein Complex
- 9O36 2.3 Å, CryoEM structure of mu-opioid receptor - Gi protein complex bound to fluornitrazene (FNZ)
- 9P80 2.3 Å, NTSR1-Gi-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
- 8XXV 2.33 Å, Cryo-EM Structure of the Prostaglandin D2 Receptor 2-indomethacin Coupled to G Protein
- 3UMS 2.34 Å, Crystal structure of the G202A mutant of human G-alpha-i1
- 3ONW 2.38 Å, Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.
- 20ZG 2.4 Å, Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the…
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