9KXS: EP67 bound mouse C5aR1

Structure of EP67 bound mouse C5aR1 in complex with Go. Determined by electron microscopy at 3.31 Å resolution. Released 26 Nov 2025.

Method: Electron microscopy
Resolution: 3.31 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 8,152
Mol. weight: 146.96 kDa
Released: 26 Nov 2025

Explore 9KXS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9KXS contains 35 α-helices and 66 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	35-65	31
α-helix	70-72	3
α-helix	73-87	15
α-helix	89-97	9
α-helix	108-110	3
α-helix	112-139	28
α-helix	141-147	7
α-helix	150-174	25
β-strand	175	1	1
β-strand	178-179	2	2
β-strand	182-183	2	2
β-strand	186	1	1
α-helix	199-208	10
α-helix	209-213	5
α-helix	214-230	17
α-helix	240-265	26
α-helix	274-280	7
α-helix	283-290	8
α-helix	292-303	12
α-helix	309-314	6

Chain B: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	9-29	21
β-strand	33-38	6	3
α-helix	46-52	7
β-strand	186-191	6	3
β-strand	196-201	6	3
α-helix	209-212	4
α-helix	213-216	4
β-strand	221-227	7	3
α-helix	246-255	10
β-strand	264-270	7	3
α-helix	272-281	10
α-helix	298-309	12
β-strand	319-323	5	3
α-helix	331-350	20

Chain C: 4 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	31-34	4
α-helix	38-39	2
β-strand	49-51	3	4
β-strand	58-61	4	5
β-strand	69	1	6
β-strand	72-74	3	5
β-strand	78-82	5	5
β-strand	83	1	6
β-strand	89-94	6	5
β-strand	102-105	4	7
β-strand	111-115	5	7
β-strand	121-125	5	7
β-strand	134-139	6	7
β-strand	146-151	6	8
β-strand	157-161	5	8
β-strand	166-168	3	8
β-strand	178-180	3	8
β-strand	187	1	9
β-strand	192	1	10
β-strand	198-202	5	10
β-strand	203	1	9
β-strand	207-212	6	10
β-strand	221-223	3	10
β-strand	229-234	6	11
β-strand	240-245	6	11
β-strand	250-254	5	11
β-strand	262-264	3	11
α-helix	272-273	2
β-strand	276-278	3	12
β-strand	284-287	4	12
β-strand	295-298	4	12
β-strand	304-306	3	12
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	336-338	3	4

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-24	16
α-helix	30-43	14
α-helix	45-47	3
α-helix	54-55	2

Chain H: 3 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	13
β-strand	11-12	2	14
β-strand	17-25	9	13
β-strand	34-39	6	15
β-strand	45-51	7	15
β-strand	58-60	3	15
β-strand	68-73	6	13
β-strand	78-84	7	13
β-strand	92-97	6	15
β-strand	111	1	15
β-strand	115-117	3	15
β-strand	118-119	2	14
β-strand	141	1	16
β-strand	146-149	4	17
β-strand	154-160	7	16
β-strand	166	1	18
β-strand	172	1	18
β-strand	174-179	6	17
β-strand	186-190	5	17
β-strand	194-195	2	17
α-helix	196	1
β-strand	203-208	6	16
β-strand	211-217	7	16
α-helix	221-223	3
β-strand	225-231	7	17
α-helix	237	1
β-strand	243-247	5	17

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Muscarinic acetylcholine receptor M4,C5a anaphylatoxin chemotactic receptor 1	A	protein	407	Homo sapiens, Mus musculus	P08173 (AlphaFold model), P30993 (AlphaFold model)
Guanine nucleotide-binding protein G(o) subunit alpha	B	protein	240	Homo sapiens	P09471 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
Antibody fragment ScFv16	H	protein	256	Mus musculus
EP67 ligand	D	protein	10	Homo sapiens

Sequence of entity 1 (A), FASTA

>9KXS_1 Muscarinic acetylcholine receptor M4,C5a anaphylatoxin chemotactic receptor 1 (chains A)
MGKTIIALSYIFCLVFADYKDDDDAANFTPVNGSSGNQSVRLVTSSSLEVLFQGPGSDPI
DNSSFEINYDHYGTMDPNIPADGIHLPKRQPGDVAALIIYSVVFLVGVPGNALVVWVTAF
EARRAVNAIWFLNLAVADLLSCLALPVLFTTVLNHNYWYFDATACIVLPSLILLNMYASI
LLLATISADRFLLVFKPIWCQKVRGTGLAWMACGVAWVLALLLTIPSFVYREAYKDFYSE
HTVCGINYGGGSFPKEKAVAILRLMVGFVLPLLTLNICYTFLLLRTWSRKATRSTKTLKV
VMAVVICFFIFWLPYQVTGVMIAWLPPSSPTLKRVEKLNSLCVSLAYINCCVNPIIYVMA
GQGFHGRLLRSLPSIIRNALSEDSVGRDSKTFTPSTTDTSTRKSQAV

Sequence of entity 2 (B), FASTA

>9KXS_2 Guanine nucleotide-binding protein G(o) subunit alpha (chains B)
MGHHHHHHENLYFQGTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGADNSGKST
IVKQMKIIHGGSGGSGGTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIF
CVDLSDYNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGP
NTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNAQVIFDAVTDIIIANNLRGCGLY

Sequence of entity 3 (C), FASTA

>9KXS_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>9KXS_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (H), FASTA

>9KXS_5 Antibody fragment ScFv16 (chains H)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQ

Sequence of entity 6 (D), FASTA

>9KXS_6 EP67 ligand (chains D)
YSFKDMPLAR

Primary citation

Structural basis of complement anaphylatoxin receptor activation by an immunostimulant lead candidate. Dalal, A., Yadav, M.K., Ganguly, M. et al. Proc Natl Acad Sci U S A (2026) 123:e2614459123-e2614459123. DOI 10.1073/pnas.2614459123 · PubMed

Other PDB entries of the same protein (UniProt P08173 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7TRP 2.4 Å, Human M4 muscarinic acetylcholine receptor complex with Gi1 and the agonist iperoxo and…
8FX5 2.45 Å, Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
7TRQ 2.5 Å, Human M4 muscarinic acetylcholine receptor complex with Gi1 and the agonist iperoxo and…
9N09 2.57 Å, Human M4 muscarinic acetylcholine receptor Gi1 complex (mini-Gsi chimera) bound to the…
5DSG 2.6 Å, Structure of the M4 muscarinic acetylcholine receptor (M4-mT4L) bound to tiotropium
9N29 2.6 Å, hM4Di(GRANPA) in complex with mGsI and diphenhydramine
8E9X 2.7 Å, CryoEM structure of miniGo-coupled hM4Di in complex with DCZ
9KVP 2.79 Å, Structure of SB290157 bound to human C3aR in complex with Go (Full map)
7TRK 2.8 Å, Human M4 muscarinic acetylcholine receptor complex with Gi1 and the agonist iperoxo
7TRS 2.8 Å, Human M4 muscarinic acetylcholine receptor complex with Gi1 and the endogenous agonist…
9KVS 2.81 Å, Structure of SB290157 bound to human C3aR in complex with Go (Receptor Ligand Focused map)
9KV6 2.88 Å, Structure of mouse TLQP21 bound to mouse C3aR in complex with Go

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