9LWC: PDB entry 9LWC

Cryo-EM structure of epinephrine bound dopamine receptor 1 D1R-M74-miniGs-Gbeta1gamma2-Nb35 complex. Determined by electron microscopy at 2.9 Å resolution. Released 18 Feb 2026.

Method: Electron microscopy
Resolution: 2.9 Å
Organisms: Homo sapiens, Vicugna pacos
Chains: 5
Atoms: 6,236
Mol. weight: 129.36 kDa
Ligands: ALE
Released: 18 Feb 2026

Explore 9LWC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9LWC contains 25 α-helices and 51 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	19-39	21
β-strand	41-47	7	1
α-helix	53-59	7
β-strand	207	1	2
β-strand	210	1	1
α-helix	214-216	3
β-strand	219-222	4	1
β-strand	224	1	2
α-helix	234-238	5
β-strand	243-248	6	1
α-helix	266-278	13
β-strand	286-291	6	1
α-helix	294-301	8
α-helix	332-349	18
β-strand	359-362	4	1
α-helix	372-390	19

Chain B: 1 helix, 28 β-strands

Element	Residues	Length	Sheet
β-strand	47-51	5	3
β-strand	60-63	4	4
β-strand	69-73	5	4
β-strand	78-83	6	4
α-helix	84-86	3
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	122-125	4	5
β-strand	135-137	3	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-193	7	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	241-245	5	8
β-strand	250-253	4	8
β-strand	262-264	3	8
β-strand	273-278	6	9
β-strand	285-289	5	9
β-strand	294-297	4	9
β-strand	304-307	4	9
β-strand	317-320	4	3
β-strand	327-330	4	3
β-strand	336-339	4	3

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	33-43	11
α-helix	45-47	3

Chain N: 0 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	10
β-strand	6-7	2	11
β-strand	18	1	12
β-strand	21-22	2	11
β-strand	23	1	13
β-strand	25	1	10
β-strand	34-39	6	14
β-strand	46	1	14
β-strand	71	1	15
β-strand	78	1	13
β-strand	80	1	15
β-strand	83	1	12
β-strand	93-98	6	14
β-strand	118	1	14
β-strand	122-123	2	14

Chain R: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	23-50	28
α-helix	59-72	14
α-helix	73-77	5
α-helix	78-87	10
α-helix	98-126	29
α-helix	128-134	7
α-helix	137-160	24
α-helix	192-199	8
α-helix	200-204	5
α-helix	205-237	33
α-helix	267-294	28
α-helix	316-319	4
α-helix	323-331	9
α-helix	337-342	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	244	Homo sapiens	A0A590UJY2 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	303	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	25	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	134	Vicugna pacos
D(1A) dopamine receptor D1R-M74	R	protein	454	Homo sapiens	P21728 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9LWC_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
TLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGSGGS
GGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALN
LFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGED
PRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCRDIIQRMHLRQY
ELLX

Sequence of entity 2 (B), FASTA

>9LWC_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
DPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRS
SWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQI
VTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGM
CRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVS
FSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLK
IWN

Sequence of entity 3 (G), FASTA

>9LWC_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
RIKVSKAAADLMAYCEAHAKEDPLL

Sequence of entity 4 (N), FASTA

>9LWC_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHH

Sequence of entity 5 (R), FASTA

>9LWC_5 D(1A) dopamine receptor D1R-M74 (chains R)
DYKDDDDKMRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFR
HLRSKVTNFFVISLAVSDLLVAVLVMPWGAVAIIAGFWPFGSFCNIWVAFDIMCVTASIL
NLCVISVDRYWAISSPFRYERKMTPKAAFILISVVWTLSVLISFIPVQLSWHKAKPTSPS
DGNATSLAETIDNCDSSLSRTYAISSSVISFYIPVAIMIVTYTRIYRIAQKQIRRIAALE
RAAVHAKNCQTTTGNGKPVECSQPESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILN
CILPFCGSGETQPFCIDSNTFIVFNWFGYANSSLNPIIYAFNADFRKAFSTLLGCYRLCP
ATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARP
LEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT

Ligands and cofactors

ID	Name	Formula	Copies
ALE	L-epinephrine	C9 H13 N O3	1

Primary citation

Cryo-EM structure of dopamine bound mut-beta2-Adrenergic Receptor-miniGs-Nb35-scFv16 complex. Zhang, X., Gao, K., Liu, X. To be published.

Other PDB entries of the same protein (UniProt A0A590UJY2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9VMG 2.82 Å, Cryo-EM structure of the spermine-bound sea lamprey TAAR348-Gs complex
21DU 2.9 Å, Cryo-EM structure of the apomorphine bound ADGRG6-Gs complex
8K4S 2.9 Å, CryoEM structure of Gq coupled MRGPRX4 with agonist DCA-3P
9DQH 2.92 Å, CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-2825
9IZG 3.04 Å, Cryo-EM structure of LPA1-Gq complex with LPA
9WQL 3.2 Å, GRM5-Gq Complex Structure
9ASB 3.4 Å, Structure of human calcium-sensing receptor in complex with chimeric Gq (miniGisq)…

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