9NT8: GLP-1(9-36)-GLP_1R-Gs complex

Cryo-EM structure of GLP-1(9-36)-GLP_1R-Gs complex. Determined by electron microscopy at 3.0 Å resolution. Released 30 Sept 2026.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, Lama glama, synthetic construct
Chains: 6
Atoms: 8,694
Mol. weight: 164.34 kDa
Released: 30 Sept 2026

Explore 9NT8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9NT8 contains 40 α-helices and 56 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	12-38	27
β-strand	40-46	7	5
α-helix	53-60	8
β-strand	207-214	8	5
β-strand	217-224	8	5
α-helix	235-237	3
β-strand	243-248	6	5
α-helix	265-277	13
α-helix	280-282	3
β-strand	286-291	6	5
β-strand	293	1	6
α-helix	294-303	10
α-helix	308-311	4
α-helix	332-349	18
β-strand	359-363	5	5
β-strand	365	1	6
α-helix	371-390	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	7
β-strand	58-63	6	8
β-strand	69-74	6	8
β-strand	78-83	6	8
β-strand	89-94	6	8
β-strand	100-105	6	9
β-strand	111-116	6	9
β-strand	121-125	5	9
β-strand	134-139	6	9
β-strand	146-153	8	10
β-strand	156-161	6	10
β-strand	165-170	6	10
β-strand	175-181	7	10
β-strand	187-192	6	11
β-strand	198-203	6	11
β-strand	207-212	6	11
β-strand	218-222	5	11
β-strand	229-234	6	12
β-strand	240-245	6	12
β-strand	250-254	5	12
β-strand	259-264	6	12
β-strand	273-278	6	13
β-strand	284-289	6	13
β-strand	294-298	5	13
β-strand	303-308	6	13
β-strand	315-320	6	7
β-strand	327-331	5	7
β-strand	336-339	4	7

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-42	13
α-helix	53-55	3

Chain N: 2 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	14
β-strand	11	1	15
β-strand	17-25	9	14
α-helix	29-31	3
β-strand	34-39	6	16
β-strand	45-51	7	16
β-strand	58-60	3	16
β-strand	68-73	6	14
β-strand	78-84	7	14
α-helix	88-90	3
β-strand	92-98	7	16
β-strand	118	1	16
β-strand	122-124	3	16
β-strand	125	1	15

Chain P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	11-27	17

Chain R: 22 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	34-52	19
α-helix	61-63	3
β-strand	83	1	1
α-helix	84-86	3
α-helix	90-94	5
β-strand	100	1	1
β-strand	101-102	2	2
β-strand	104	1	3
β-strand	110	1	3
β-strand	112	1	4
α-helix	113	1
β-strand	119	1	4
α-helix	120	1
β-strand	121-122	2	2
α-helix	138-168	31
α-helix	170-172	3
α-helix	175-206	32
α-helix	215-222	8
α-helix	224-255	32
α-helix	262-274	13
α-helix	276-285	10
α-helix	286-291	6
α-helix	295-297	3
α-helix	303-337	35
α-helix	345-360	16
α-helix	363-367	5
α-helix	381-392	12
α-helix	394-399	6
α-helix	400-404	5
α-helix	407-419	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Glucagon-like peptide 1 receptor	R	protein	491	Homo sapiens	P43220 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	58	Homo sapiens	P59768 (AlphaFold model)
Nb35	N	protein	128	Lama glama
Glucagon-like peptide 1(7-36)	P	protein	28	synthetic construct	P01274

Sequence of entity 1 (R), FASTA

>9NT8_1 Glucagon-like peptide 1 receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPARPQGATVSLWETVQKWREYRRQCQRSLT
EDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWL
QKDNSSLPWRDLSECEESKRGERSSPEEQLLFLYIIYTVGYALSFSALVIASAILLGFRH
LHCTRNYIHLNLFASFILRALSVFIKDAALKWMYSTAAQQHQWDGLLSYQDSLSCRLVFL
LMQYCVAANYYWLLVEGVYLYTLLAFSVFSEQWIFRLYVSIGWGVPLLFVVPWGIVKYLY
EDEGCWTRNSNMNYWLIIRLPILFAIGVNFLIFVRVICIVVSKLKANLMCKTDIKCRLAK
STLTLIPLLGTHEVIFAFVMDEHARGTLRFIKLFTELSFTSFQGLMVAILYCFVNNEVQL
EFRKSWERWRLEHLHIQRDSSMKPLKCPTSSLSSGATAGSSMYTATCQASCSPAGLEVLF
QGPHHHHHHHH

Sequence of entity 2 (A), FASTA

>9NT8_2 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 3 (B), FASTA

>9NT8_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>9NT8_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
NTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFR

Sequence of entity 5 (N), FASTA

>9NT8_5 Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSS

Sequence of entity 6 (P), FASTA

>9NT8_6 Glucagon-like peptide 1(7-36) (chains P)
EGTFTSDVSSYLEGQAAKEFIAWLVKGR

Primary citation

cryo-EM structure of GLP-1(9-36)-GLP_1R-Gs complex. Zhang, X., Belousoff, M.J., Sexton, P.M. et al. To be published.

Other PDB entries of the same protein (UniProt P43220 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4ZGM 1.8 Å, Crystal structure of Semaglutide peptide backbone in complex with the GLP-1 receptor…
5OTU 1.8 Å, Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Hcs/Thr11Hcs
5OTT 1.92 Å, Extracellular domain of GLP-1 receptor in complex with exendin-4 variant Gly2Hcs/Thr5Hcs
5E94 2.0 Å, Antibody-bound Glucagon-like Peptide-1 receptor extracellular domain
5OTV 2.0 Å, Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Cyc/Thr11Hcs
5OTX 2.0 Å, Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Cys/Thr11Cys
3C5T 2.1 Å, Crystal structure of the ligand-bound glucagon-like peptide-1 receptor extracellular…
3IOL 2.1 Å, Crystal structure of Glucagon-Like Peptide-1 in complex with the extracellular domain of…
5OTW 2.1 Å, Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Hcs/Thr11Cys
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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