9NZH: Amylin-NHO-18 binder complex

Crystal Structure of Amylin-NHO-18 binder complex. Determined by X-ray diffraction at 2.03 Å resolution. Released 14 May 2025.

Method: X-ray diffraction
Resolution: 2.03 Å
Organisms: synthetic construct, Homo sapiens
Chains: 2
Atoms: 1,424
Mol. weight: 19.85 kDa
Released: 14 May 2025

Explore 9NZH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9NZH contains 8 α-helices and 4 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	2-12	11
α-helix	13-17	5
α-helix	18-31	14
β-strand	35-41	7	1
α-helix	48-66	19
β-strand	70-76	7	1
α-helix	81-99	19
β-strand	102-108	7	1
α-helix	110-113	4
α-helix	115-134	20

Chain B: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
α-helix	145-157	13
β-strand	166-172	7	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Amylin-NHO-18 Binder	A	protein	140	synthetic construct
Islet amyloid polypeptide	B	protein	37	Homo sapiens	P10997 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9NZH_1 Amylin-NHO-18 Binder (chains A)
NEEEALEKLKKYLKENEKPKLEEAKKEAEEKGKPIVHLITVEPDFPSKELLIKALTLAIK
FAKEIANIKAAVVLLAHEGSREEAEKEAKEIQEALTKETGIEVIVVGVTPEDGDEEKIQE
LANEIKNKLSKILHGKEYDP

Sequence of entity 2 (B), FASTA

>9NZH_2 Islet amyloid polypeptide (chains B)
KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY

Primary citation

Diffusing protein binders to intrinsically disordered proteins. Liu, C., Wu, K., Choi, H. et al. Nature (2025) 644:809-817. DOI 10.1038/s41586-025-09248-9 · PubMed

Other PDB entries of the same protein (UniProt P10997 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8YG2 1.25 Å, Crystal structure of amyloidogenic peptide Piv-NFGAIL-NH2 from Islet Amyloid Polypeptide
5KO0 1.4 Å, Human Islet Amyloid Polypeptide Segment 15-FLVHSSNNFGA-25 Determined by MicroED
3FPO 1.5 Å, HSSNNF segment from Islet Amyloid Polypeptide (IAPP or Amylin)
3FTK 1.5 Å, NVGSNTY segment from Islet Amyloid Polypeptide (IAPP or Amylin), hydrated crystal form
3FTL 1.6 Å, NVGSNTY segment from Islet Amyloid Polypeptide (IAPP or Amylin), dehydrated crystal form
3FTR 1.61 Å, Structure of an amyloid forming peptide SSTNVG from IAPP (alternate polymorph)
3DG1 1.66 Å, Segment SSTNVG derived from IAPP
3G7W 1.75 Å, Islet Amyloid Polypeptide (IAPP or Amylin) Residues 1 to 22 fused to Maltose Binding…
3FTH 1.84 Å, NFLVHSS segment from Islet Amyloid Polypeptide (IAPP or Amylin)
3FR1 1.85 Å, NFLVHS segment from Islet Amyloid Polypeptide (IAPP or Amylin)
3G7V 1.86 Å, Islet Amyloid Polypeptide (IAPP or Amylin) fused to Maltose Binding Protein
9CC5 1.87 Å, De novo design of high-affinity protein binders to bio active peptide Amylin

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