Mitotic checkpoint serine/threonine-protein kinase BUB1 (BUB1) is a 1085-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: O43683.
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The mean pLDDT of this model is 62.6 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 30% |
| 70 to 90 | Confident: backbone generally right | 19% |
| 50 to 70 | Low: treat with caution | 7% |
| Below 50 | Very low: often disordered regions | 45% |
What pLDDT means and how to read it
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Promotes the centromeric localization of TOP2A (PubMed:35044816). Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex…
Interacts with BUB3 and KNL1. Interacts (when phosphorylated) with PLK1. The BUB1-BUB3 complex interacts with MAD1L1
Nucleus, Chromosome, centromere, kinetochore
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 7B1F | X-ray | 1.75 Å | C/D=455-479 |
| 6F7B | X-ray | 2.0 Å | A=726-1085 |
| 4QPM | X-ray | 2.2 Å | A/B=740-1085 |
| 4R8Q | X-ray | 2.31 Å | A=724-1085 |
| 5DMZ | X-ray | 2.4 Å | A/B=726-1085 |
| 7B1H | X-ray | 2.4 Å | C/D/G/H=455-479 |
| 4A1G | X-ray | 2.6 Å | A/B/C/D=1-150 |
| 7B1J | X-ray | 2.9 Å | C/D=455-479 |
| 2LAH | NMR | A=1-150 |
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