Non-structural protein 1 (NS) is a 230-residue protein from Influenza A virus. This is its AlphaFold structure prediction, created 3 Sept 2026. UniProt accession: P03496.
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The mean pLDDT of this model is 53.7 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 4% |
| 70 to 90 | Confident: backbone generally right | 26% |
| 50 to 70 | Low: treat with caution | 20% |
| Below 50 | Very low: often disordered regions | 50% |
What pLDDT means and how to read it
Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.…
Homodimer. Interacts with host TRIM25 (via coiled coil); this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated RIGI CARD ubiquitination. Interacts with human EIF2AK2/PKR, CPSF4, IVNS1ABP and PABPN1
Host nucleus, Host cytoplasm
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 2ZKO | X-ray | 1.7 Å | A/B=1-70 |
| 3RVC | X-ray | 1.8 Å | A=79-230 |
| 3O9R | X-ray | 2.0 Å | A/B=79-230 |
| 2GX9 | X-ray | 2.1 Å | A/B=79-207 |
| 3O9T | X-ray | 2.2 Å | A/B=79-230 |
| 3L4Q | X-ray | 2.3 Å | A/B=73-230 |
| 3O9S | X-ray | 2.48 Å | A/B=79-230 |
| 3O9Q | X-ray | 2.5 Å | A/B=79-230 |
| 5NT1 | X-ray | 2.82 Å | B/F/J=80-230 |
| 3O9U | X-ray | 3.2 Å | A/B/C/D/E/F/G/H=79-230 |
| 5NT2 | X-ray | 4.26 Å | C/D/E/F=1-230 |
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