Regulatory protein GAL4 (GAL4) is a 881-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P04386.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 66.0 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 31% |
| 70 to 90 | Confident: backbone generally right | 26% |
| 50 to 70 | Low: treat with caution | 9% |
| Below 50 | Very low: often disordered regions | 35% |
What pLDDT means and how to read it
This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes
Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17
Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3COQ | X-ray | 2.4 Å | A/B=8-96 |
| 1D66 | X-ray | 2.7 Å | A/B=1-65 |
| 3BTS | X-ray | 2.7 Å | E/F=854-874 |
| 7UIO | EM | 3.3 Å | GA/GB=1-147 |
| 7UIK | EM | 7.7 Å | T/U=1-147 |
| 1AW6 | NMR | A=1-43 | |
| 1HBW | NMR | A/B=50-106 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.