Type 3 secretion system translocon protein SctE (sctE) is a 580-residue protein from Shigella flexneri. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P18011.
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The mean pLDDT of this model is 48.8 (very low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 4% |
| 70 to 90 | Confident: backbone generally right | 12% |
| 50 to 70 | Low: treat with caution | 22% |
| Below 50 | Very low: often disordered regions | 62% |
What pLDDT means and how to read it
Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:10545510, PubMed:17367391). IpaB/SctE and IpaC/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells (PubMed:10545510, PubMed:17367391, PubMed:34809452). Interaction with IpaD/SctA at needle tips leads to the formation of the MxiH/SctF-IpaD/SctA-IpaB/SctE ternary complex, which is essential for host cell sensing (PubMed:17367391, PubMed:19433542). Interaction of IpaB/SctE with host membrane lipids promotes recruitment of IpaC/SctB at the…
The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569). This subunit is involved in the formation of a pore, called the translocon, in host membrane (PubMed:10545510, PubMed:17367391, PubMed:34809452). Interacts with IpaC/SctB (PubMed:10545510, PubMed:17367391,…
Secreted, Host membrane, Host cell, Host nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3U0C | X-ray | 2.05 Å | A/B=28-226 |
| 5WKQ | X-ray | 2.1 Å | A/B=74-242 |
| 3GZ1 | X-ray | 2.15 Å | P/Q=51-72 |
| 6KEA | X-ray | 2.35 Å | A/B/C/D=49-76 |
| 3GZ2 | X-ray | 2.65 Å | P=16-72 |
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