Tubulin beta-4B chain (TUBB4B) is a 445-residue protein from Bos taurus. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q3MHM5.
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The mean pLDDT of this model is 91.9 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 82% |
| 70 to 90 | Confident: backbone generally right | 14% |
| 50 to 70 | Low: treat with caution | 3% |
| Below 50 | Very low: often disordered regions | 1% |
What pLDDT means and how to read it
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:2207090, PubMed:6504138, PubMed:7704569). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:2207090, PubMed:6504138, PubMed:7704569)
Dimer of alpha and beta chains (PubMed:2207090, PubMed:6504138, PubMed:7704569). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural…
Cytoplasm, cytoskeleton, Cytoplasm, cytoskeleton, flagellum axoneme
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 7RRO | EM | 3.4 Å | AB/AD/AF/AH/AJ/AL/BB/BD/BF/BH/BJ/BL/CB/CD/CF/CH/CJ/CL/DB/DD/DF/DH/DJ/DL/DN/EB/ED/EF/EH/EJ=1-445 |
| 8OU0 | EM | 3.5 Å | B=1-445 |
| 9CPB | EM | 3.52 Å | AB/AD/AF/AH/AJ/AL/BB/BD/BF/BH/BJ/BL/CB/CD/CF/CH/CJ/CL/DB/DD/DF/DH/DJ/DL/ED/EF/EH/EJ/EL/FD=1-445 |
| 8OTZ | EM | 3.6 Å | AB/AD/AF/AH/AJ/AL/BB/BD/BF/BH/BJ/BL/CB/CD/CF/CH/CJ/CL/DB/DD/DF/DH/DJ/DL/DN/EB/ED/EF/EH/EJ=1-445 |
| 9FQR | EM | 5.0 Å | AB/AD/AF/AH/AJ/AL/AM/AO/AX/BB/BD/BF/BH/BJ/BL/Bp/Bv/Bx/CB/CD/CF/CH/CJ/CL/DB/DD/DF/DH/DJ/DL=1-445 |
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