E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) is a 975-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q96PU5.
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The mean pLDDT of this model is 68.4 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 29% |
| 70 to 90 | Confident: backbone generally right | 29% |
| 50 to 70 | Low: treat with caution | 10% |
| Below 50 | Very low: often disordered regions | 32% |
What pLDDT means and how to read it
E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair (PubMed:20064473, PubMed:31959741, PubMed:33608556). Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation (PubMed:15496141). Down-regulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels (PubMed:28820317, PubMed:31959741). Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5,…
Interacts with UBE2E3 (By similarity). Interacts with NDFIP1; this interaction activates the E3 ubiquitin-protein ligase (PubMed:11748237, PubMed:26363003). Interacts with NDFIP2; this interaction activates the E3 ubiquitin-protein ligase (PubMed:26363003). Interacts (via WW domains) with SCN1A (By similarity). Interacts (via WW domains) with SCN2A (PubMed:15548568). Interacts (via WW domains)…
Cytoplasm, Golgi apparatus, Endosome, multivesicular body
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 7LP1 | X-ray | 1.35 Å | A=494-532 |
| 7LP3 | X-ray | 1.61 Å | A/C=193-226 |
| 6ZBT | X-ray | 1.8 Å | E/F/G/H=338-347 |
| 2NSQ | X-ray | 1.85 Å | A=1-154 |
| 7LP2 | X-ray | 1.88 Å | A/C/E=385-418 |
| 6ZC9 | X-ray | 1.9 Å | E/F/G/H=444-453 |
| 2ONI | X-ray | 2.2 Å | A=594-967 |
| 7NMZ | X-ray | 2.3 Å | C=335-455 |
| 5HPK | X-ray | 2.43 Å | A=594-975 |
| 9Z5Q | EM | 3.06 Å | B=541-975 |
| 3JW0 | X-ray | 3.1 Å | C/D=596-975 |
| 3JVZ | X-ray | 3.3 Å | C/D=596-975 |
| 9GIK | EM | 3.58 Å | A=1-975 |
| 9GIM | EM | 4.11 Å | A=1-975 |
| 2LAJ | NMR | A=496-535 | |
| 2LB2 | NMR | A=386-420 | |
| 2LTY | NMR | A=385-417 | |
| 2MPT | NMR | A=496-539, B=945-957 | |
| 7LP4 | NMR | A=493-539 | |
| 7LP5 | NMR | A=493-539 |
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