Serine/threonine-protein kinase WNK3 (WNK3) is a 1800-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9BYP7.
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The mean pLDDT of this model is 47.4 (very low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 14% |
| 70 to 90 | Confident: backbone generally right | 12% |
| 50 to 70 | Low: treat with caution | 4% |
| Below 50 | Very low: often disordered regions | 70% |
What pLDDT means and how to read it
Serine/threonine-protein kinase component of the WNK3-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress (PubMed:16275911, PubMed:16275913, PubMed:16501604, PubMed:22989884, PubMed:36318922). WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation (PubMed:36318922). The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent…
Interacts with WNK1 and WNK4
Cytoplasm
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5O1V | X-ray | 1.72 Å | A/B=132-414 |
| 5O2B | X-ray | 2.04 Å | A/B=132-414 |
| 5O21 | X-ray | 2.06 Å | A/B=132-414 |
| 5O23 | X-ray | 2.25 Å | A/B=132-414 |
| 5O26 | X-ray | 2.38 Å | A/B=133-414 |
| 5O2C | X-ray | 2.4 Å | A=123-500 |
| 5NKP | X-ray | 2.8 Å | C/D=537-547 |
| 8EDH | X-ray | 3.11 Å | A/C=118-409 |
| 9D7Q | X-ray | 3.3 Å | A/B=130-409 |
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