5O1V: Serine/threonine-protein kinase WNK3

Crystal structure of WNK3 kinase domain in a monophosphorylated apo state (A-loop swapped). Determined by X-ray diffraction at 1.72 Å resolution. Released 28 Jun 2017.

Method: X-ray diffraction
Resolution: 1.72 Å
Organism: Homo sapiens
Chains: 2
Atoms: 4,873
Mol. weight: 66.46 kDa
Released: 28 Jun 2017

Explore 5O1V in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5O1V contains 36 α-helices and 20 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	137-140	4	1
β-strand	146-156	11	1
β-strand	159-166	8	1
β-strand	172-179	8	1
α-helix	180	1
α-helix	185-200	16
β-strand	206	1	2
β-strand	209-215	7	1
α-helix	217-219	3
β-strand	222-228	7	1
β-strand	234	1	2
α-helix	235-239	5
α-helix	247-265	19
α-helix	270-271	2
α-helix	278-280	3
β-strand	281-284	4	2
β-strand	289-292	4	2
α-helix	297-301	5
α-helix	313-315	3
α-helix	318-321	4
α-helix	328-343	16
α-helix	354-362	9
α-helix	365-367	3
α-helix	368-372	5
α-helix	376-385	10
α-helix	390-392	3
α-helix	394-395	2
α-helix	396-400	5
α-helix	403-405	3

Chain B: 17 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	137-140	4	3
β-strand	146-156	11	3
β-strand	159-166	8	3
β-strand	172-178	7	3
α-helix	179-180	2
α-helix	185-198	14
β-strand	206	1	4
β-strand	209-217	9	3
β-strand	220-228	9	3
β-strand	234	1	4
α-helix	235-239	5
α-helix	247-265	19
α-helix	278-280	3
β-strand	281-284	4	4
β-strand	289-292	4	4
α-helix	297-300	4
α-helix	313-315	3
α-helix	318-321	4
α-helix	328-343	16
α-helix	354-363	10
α-helix	365-367	3
α-helix	368-372	5
α-helix	376-385	10
α-helix	390-392	3
α-helix	394-395	2
α-helix	396-400	5
α-helix	403-405	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Serine/threonine-protein kinase WNK3	A, B	protein	285	Homo sapiens	Q9BYP7 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>5O1V_1 Serine/threonine-protein kinase WNK3 (chains A, B)
SMEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQ
RFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKV
LRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSV
IGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPAS
FNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVE

Primary citation

Crystal structure of WNK3 kinase domain in a monophosphorylated apo state (A-loop swapped). Pinkas, D.M., Daubner, G.M., Bufton, J.C. et al. To be published.

Other PDB entries of the same protein (UniProt Q9BYP7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5O2B 2.04 Å, Crystal structure of WNK3 kinase domain in a diphosphorylated state and in a complex…
5O21 2.06 Å, Crystal structure of WNK3 kinase domain in a monophosphorylated state with chloride…
5O23 2.25 Å, Crystal structure of WNK3 kinase domain in a monophosphorylated apo state
5O26 2.38 Å, Crystal structure of WNK3 kinase domain in a diphosphorylated state and in complex with…
5O2C 2.4 Å, Crystal structure of WNK3 kinase and CCT1 didomain in a unphosphorylated state
5NKP 2.8 Å, Crystal structure of the human KLHL3 Kelch domain in complex with a WNK3 peptide
8EDH 3.11 Å, Identification of a class of WNK isoform-specific inhibitors through high-throughput…
9D7Q 3.3 Å, Water and chloride as allosteric inhibitors in WNK kinase osmosensing

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